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初級會員·12年您現在的位置： 翌圣生物科技（上海）股份有限公司>>分子生物學>> 11811023G418 Sulfate（Geneticin）
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產品簡介
產品介紹
現貨供應Gibco公司G418產品
產品介紹：
G418（G-418即G-418 sulfate，也稱Geneticin，分子式為C20H40N4O10.2H2SO4，分子量為692.71 g/mole）是一種氨基糖類抗生素，其結構與新霉素、慶大霉素、卡那霉素相似，它通過影響80S核糖體功能而阻斷蛋白質合成,對原核和真核等細胞都有毒性 ,包括細菌、酵母、植物和哺乳動物細胞 ,也包括原生動物和蠕蟲。是穩定轉染zui常用的選擇試劑。當neo基因被整合進真核細胞基因組合適的地方后 ,則能啟動neo基因編碼的序列轉錄為mRNA,從而獲得抗性產物氨基糖苷磷酸轉移酶的高效表達 ,使細胞獲得抗性而能在含有Ｇ418的選擇性培養基中生長。Ｇ418的這一選擇特性 ,已在基因轉移、基因敲除、抗性篩選以及轉基因動物等方面得以廣泛應用。
G418和篩選
篩選之前由于每種細胞對G418的敏感性不同，而且不同的廠家生產的相同濃度的G418的活性不盡相同，所以在篩選之前，一定要確定G418的*篩選濃度。具體如下：將細胞稀釋到1000個細胞/mL，在100ug/mL~1mg/mL的G418濃度范圍內進行篩選，選擇出在10~14天內使細胞全部死亡的zui低G418濃度來進行下一步的篩選試驗。
由于每種細胞對G418的敏感性不同，一般變動在100ug/ml～1000ug/ml范圍。而且不同的廠家生產的相同濃度的G418的活性不盡相同，所以在篩選之前，一定要確定你的細胞對這一批G418的*篩選濃度。盡管如此，特性明確的細胞系G418的*用量還是穩定的。《分子克隆》給出了幾個常用細胞系所需G418的*用量：
細胞系或機體 | G418濃度（ug/ml） |
中國倉鼠卵巢細胞 | 700～800 |
Madin-Darby犬腎細胞 | 500 |
人上皮A431細胞 | 400 |
猿CV-1細胞 | 500 |
盤基網柄菌屬 | 10～35 |
植物 | 10 |
酵母 | 125～500 |
訂購信息：
貨號 | 名稱 | 產地 | CAS 號碼 | 規格 | 報價（元） | *（元） |
G418 Sulfate（Geneticin） | Mpbio | 108321-42-2 | 1g | 2298 | 345 | |
G418 Sulfate（Geneticin） | Mpbio | 108321-42-2 | 5g | 5895 | 1450 | |
11811023 | Gibco | 108321-42-2 | 1g | 558 | 380 | |
11811031 | Gibco | 108321-42-2 | 5g | 4109 | 1580 | |
60220ES03 | YEASEN | 108321-42-2 | 1g | 358 | 358 | |
60220ES08 | YEASEN | 108321-42-2 | 5g | 1480 | 1480 |
我公司提供的G418來自Gibco公司，技術參數如下：
Shipping Conditions（運輸條件）
GENETICIN is shipped at ambient temperature.
Storage Conditions（儲存環境）
GENETICIN should be stored at room temperature, 15 to 30°C. GENETICIN is soluble in water and should be stored at 2 to 8°C as a solution.
Background
GENETICIN is an aminoglycoside related to Gentamicin and is used as a selective agent in molecular genetics experiments. It is toxic to both prokaryotic and eukaryotic cells. The resistance genes are dominant and are located on both Transposons Tn601 （903） and Tn5. The gene on Tn5 is referred to as a aminoglycoside phosphotransferase 3' （I） or APH（3'）I.The genes are bacterial in derivation but they can be expressed in eukaryotic cells. Efficient expression is achieved when the neo DNA sequence is linked to eukaryotic DNA sequences that permit transcription and processing of the Neo coding sequence into mRNA. Introduction of either of these genes into cells can confer resistance to GENETICIN, enabling the cells to grow in media containing GENETICIN.
Description
GENETICIN is also known as Antibiotic G-418 Sulfate. GENETICIN is supplied
as a white powder. The amount of GENETICIN required to be present in culture media to select for resistant cells varies with a number of factors including cell type. Good laboratory practice requires that the optimal concentration of GENETICIN required to maintain and select cells must be determined for each set of growth conditions. Invitrogen recommends that whenever experimental conditions are altered （including use of GENETICIN from a different lot）, the optimal concentration of the product be re-evaluated.
Quality Control
Absorbance - Visible range: At a concentration of 100 mg/mL in water, GENETICIN gives a clear colorless solution. The absorbance of this solution at 400 nm is less than 0.075 O.D.
Absorbance - Ultraviolet range: At a concentration of 1 mg/mL in water, the absorbance at 280 nm is less than 0.015 O.D.
Oxoid專題 Novagen專題 Stratagene專題 糖尿病建模專題 常備試劑* |
