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CREB luciferase reporter plasmid熒光素酶報(bào)告基因細(xì)胞核內(nèi)調(diào)控因子
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REPOTMCREB報(bào)告基因主要用于檢測(cè)細(xì)胞中cAMP/PKA信號(hào)通路的活性、藥物研究以及基因過(guò)表達(dá)和RNAi的表型分析等。
PGMCRE-Lu是吉滿生物改造后的哺乳動(dòng)物真核表達(dá)載體,在其多克隆位點(diǎn)插入了多個(gè)CRE結(jié)合位點(diǎn),可以高靈敏度地檢測(cè)CREB的激活水平。同時(shí),對(duì)載體中預(yù)測(cè)出的其它轉(zhuǎn)錄因子以外的結(jié)合位點(diǎn)進(jìn)行了適當(dāng)?shù)耐蛔儯黾恿速|(zhì)粒的轉(zhuǎn)錄因子結(jié)合特異性。由于質(zhì)粒體積減小,使得CREB報(bào)告基因更易于轉(zhuǎn)染。
質(zhì)粒圖譜:
質(zhì)粒元件信息:
CRE response element (CRE) | 32-63 |
Minimal TA promoter (pTA) | 92-114 |
Luciferase reporter gene | 146-1808 |
SV40 late poly(A) signal | 1843-2064 |
SV40 early promoter | 2112-2530 |
Synthetic neomycin phosphotransferase(Neor) coding region | 2555-3349 |
Synthetic poly(A) signal | 3374-3422 |
Synthetic Beta-lactamase(Ampr) coding region | 4537-5397 |
Synthetic poly(A) signal/transcriptional pause site | 5502-5655 |
包裝清單:
產(chǎn)品編號(hào) | 產(chǎn)品名稱(chēng) | 包裝 |
GM-021030 | REPOTMCREB報(bào)告基因 | 1μg |
- | 說(shuō)明書(shū) | - |
使用說(shuō)明:
pGMCRE-Lu可以采用常規(guī)轉(zhuǎn)染法轉(zhuǎn)染哺乳動(dòng)物細(xì)胞。用熒光素酶檢測(cè)試劑盒或雙熒光素酶檢測(cè)試劑盒進(jìn)行檢測(cè)。
CRE的激活劑,可作為CRE報(bào)告基因的陽(yáng)性對(duì)照。
注意事項(xiàng):
本質(zhì)粒未經(jīng)吉滿生物允許不得用于任何商業(yè)用途,也不得移交給訂貨人實(shí)驗(yàn)室以外的任何人或單位。
為了您的健康,實(shí)驗(yàn)操作時(shí)請(qǐng)穿實(shí)驗(yàn)服和帶一次性手套。
保存條件:
-20℃保存。
參考文獻(xiàn):
[1] Wang Y, Meng F, Zhong Y, Huang G, Li J.
Discovery of a novel glucagon-like peptide (GCGL) and its receptor (GCGLR) in chickens: evidence for the existence of GCGL and GCGLR genes in nonmammalian vertebrates.
Endocrinology. 2012 Nov;153(11):5247-60. doi: 10.1210/en.2012-1586. Epub 2012 Sep 26.
[2] Carriba P, Pardo L, Parra-Damas A, Lichtenstein MP, Saura CA, Pujol A, Masgrau R, Galea E.
ATP and noradrenaline activate CREB in astrocytes via noncanonical Ca(2+) and cyclic AMP independent pathways.
Glia. 2012 Sep;60(9):1330-44. doi: 10.1002/glia.22352. Epub 2012 May 16.
[3] Huang G, Li J, Fu H, Yan Z, Bu G, He X, Wang Y.
Characterization of glucagon-like peptide 1 receptor (GLP1R) gene in chickens: functional analysis, tissue distribution, and identification of its transcript variants.
Domest Anim Endocrinol. 2012 Jul;43(1):1-15. doi: 10.1016/j.domaniend.2012.01.005. Epub 2012 Feb 22.
[4] Wang Y, Huang G, Li J, Meng F, He X, Leung FC.
Characterization of chicken secretin (SCT) and secretin receptor (SCTR) genes: a novel secretin-like peptide (SCT-LP) and secretin encoded in a single gene.
Mol Cell Endocrinol. 2012 Jan 2;348(1):270-80. doi: 10.1016/j.mce.2011.09.012. Epub 2011 Sep 14.
[5] Larabee JL, Maldonado-Arocho FJ, Pacheco S, France B, DeGiusti K, Shakir SM, Bradley KA, Ballard JD.
Glycogen synthase kinase 3 activation is important for anthrax edema toxin-induced dendritic cell maturation and anthrax toxin receptor 2 expression in macrophages.
Infect Immun. 2011 Aug;79(8):3302-8. doi: 10.1128/IAI.05070-11. Epub 2011 May 16.
產(chǎn)品價(jià)格:
貨號(hào) | 名稱(chēng) | 產(chǎn)地 | 規(guī)格 | 報(bào)價(jià)/元 |
GM021030 | CREB luciferase reporter plasmid 熒光素酶報(bào)告基因(報(bào)告基因質(zhì)粒)細(xì)胞核內(nèi)調(diào)控因子 | Genomeditech | 1μg | 1500 |