貓杯狀病毒IgG免疫熒光玻片

Feline Calicivirus IgG IFA Substrate slide

廣州健侖生物科技有限公司

主要用途：用于檢測貓血清中貓杯狀病毒IgG抗體

產品規格：12 孔/張,10 張/盒

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貓杯狀病毒IgG免疫熒光玻片

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【公司名稱】 廣州健侖生物科技有限公司
【】    楊永漢 
【】 
【騰訊 】 2042552662
【公司地址】 廣州清華科技園創新基地番禺石樓鎮創啟路63號二期2幢101-3室
【企業文化】

由于脂肪的存在，厚組織都是不透光的。要進行成像就得把組織切得特別薄，問題是這樣就無法獲得組織的3D結構。近年來出現了一些通過去除脂肪讓組織變透明的方法，不過這些方法大多需要在組織透明化之前，構建和表達發熒光的目標蛋白。隨后，斯坦福大學的Karl Deisseroth開發出CLARITY技術，該技術允許人們在組織透明化之后，使用標記抗體或核酸探針，大大拓展了組織透明化的應用。不過CLARITY處理組織仍有大小限制，Deisseroth說。
在本研究中，文章的資深作者Viviana Gradinar將自己的全身組織透明化技術稱為PARS(perfusion assisted agent release in situ)。該技術在CLARITY技術的基礎上，將透明劑持續泵入動物的循環系統(或者通過腦脊液管道泵入大腦)，讓試劑逐漸進入組織。Gradinaru等人利用PARS建立了幾乎*透明的小鼠和小鼠器官，并用熒光抗體在其中揭示不同的細胞和結構。
“這是一次重要的技術進步，”麻省大學的Guangping Gao教授評論道，他的研究方向是開發腺病毒相關載體用于基因治療。“可以為我們揭示病毒如何穿過血管進入細胞，以及宿主如何與病毒載體相互作用。”
Gradinaru指出，PARS特別適合用來觀察機體中的長神經元。“這一方法可以幫助人們定位周圍神經系統，”她說。
PARS可以同時定位DNA、RNA和蛋白，“但仍然受到抗體擴散能力的限制，”Gradinaru說。因此在使用這一技術時，使用納米抗體等較小的標記。另外，數據處理也是PARS面臨的一大挑戰。
日本RIKEN和東京大學的科學家們，將組織脫色與光切熒光顯微鏡結合起來，成像了多種器官乃至整個生物體，得到了極為詳細的內部圖像。這一成果發表在本期的Cell雜志上。理解生命的運作方式是一直系統生物學的*夢想。將組織和生物體透明化然后進行單細胞分辨率的精確成像，將成為實現這一夢想的全新途徑。
研究人員在這項研究中采用了一種名為CUBIC（Clear, Unobstructed Brain Imaging Cocktails and Computational Analysis）的方法，他們之前曾用這個方法成像了整個大腦。大腦組織富含脂類比較容易透明化，但機體其他部分含有許多能夠吸收光的生色團（chromophore），比如血紅素heme。血紅素是血紅蛋白的重要組分，存在于機體的絕大多數組織，并且會阻斷光線。

Thick tissues are opaque due to the presence of fat. For imaging, the tissue must be cut so thin that the problem is that the 3D structure of the tissue can not be obtained. In recent years there have been some ways to make tissues transparent by removing fat, but most of these methods require the construction and expression of a fluorescent target protein before the tissue is transparent. Subsequently, Karl Deisseroth of Stanford University developed CLARITY technology, which allows people to use labeled antibodies or nucleic acid probes after tissue is transparent, greatly expanding the use of tissue transparency. However, CLARITY processing organizations still have size restrictions, Deisseroth said.
In the present study, Viviana Gradinar, a senior author of the article, described his whole-body tissue opacification technique as PARS (perfusion assisted agent release in situ). Based on CLARITY technology, this technology pumped clear agent continuously into the animal's circulatory system (or into the brain through the cerebrospinal fluid) to allow the gradual entry of the agent into the tissue. Gradinaru et al. Used PARS to establish almost compley transparent mouse and mouse organs, revealing different cells and structures with fluorescent antibodies.
"This is an important technological advance," said Professor Guangping Gao at the University of Massachusetts. His research interests include the development of adenovirus-associated vectors for gene therapy. "It shows us how the virus goes through the bloodstream into the cell and how the host interacts with the viral vector."
Gradinaru notes that PARS is particularly well suited for observing long neurons in the body. "This method helps people to locate the peripheral nervous system," she said.
PARS can position DNA, RNA and proteins simultaneously, "but is still limited by the ability of antibodies to proliferate," said Gradinaru. Therefore, in the use of this technology, it is best to use smaller labels such as Nanobodies. In addition, data processing is also a major challenge facing PARS.
Scientists at Japan's RIKEN and the University of Tokyo combined tissue decolorization with light-cut fluorescence microscopy to image a wide range of organs and even entire organisms and obtain extremely detailed internal images. This result is published in the current issue of Cell magazine. Understanding the way life works is the ultimate dream of systems biology. Transparency of tissues and organisms followed by precise imaging of single-cell resolution will be a new way to achieve this dream.
In this study, the researchers used a method called CUBIC (Clear, Unobstructed Brain Imaging Cocktails and Computational Analysis), which they had previously used to image the entire brain. Cerebral tissue is more lipid-rich and more transparent, but the rest of the body contains many chromophores that absorb light, such as heme. Heme is an important component of hemoglobin, exists in the vast majority of the body's tissues, and will block the light.