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PIERCE,BCA Protein Assay Kit- Reducing Agent Compa

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產(chǎn)品型號(hào)23250

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更新時(shí)間:2024-05-31 12:46:29瀏覽次數(shù):1293次

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貨號(hào):23250,名稱: BCA Protein Assay Kit- Reducing Agent Compatible ,品牌:PIERCE,規(guī)格:KIT

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BCA Protein Assay Kit- Reducing Agent Compatible介紹

Thermo Scientific BCA蛋白定量產(chǎn)品可以用于研究蛋白:蛋白間相互作用,衡量親和層析后的組分含量,估算從細(xì)胞裂解液里提取的膜蛋白含量和高通量篩選融合蛋白。


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BCA Protein Assay Kit- Reducing Agent Compatible品牌介紹

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BCA Protein Assay Kit- Reducing Agent Compatible僅用于科研


This reducing agent-compatible (RAC) version of the Thermo Scientific Pierce BCA Protein Assay provides accurate determination of protein concentration in samples that contain dithiothreitol (DTT), 2-mercaptoethanol (BME), TCEP and other disulfide reducing agents.

The BCA Protein Assay Kit – Reducing Agent Compatible (BCA-RAC) provides all of the advantages of the original BCA Assay, plus compatibility with disulfide reducing agents at concentrations routinely used in protein sample buffers. This special adaptation of the popular Pierce BCA Protein Assay method enables accurate protein concentration measurement for samples containing DTT, 2-ME or TCEP. Available in tube and microplate formats, these reducing agent compatible (RAC) BCA Kits extend the already broad reagent compatibility of the BCA Protein Assay protocol to include nearly all types of components commonly present in protein research samples.

Highlights:

  • Compatible – assay samples that contain up to 5mM DTT, 35mM BME, or 10mM TCEP (see complete Protein Assay Compatibility Table)
  • BCA technology – only a slight modification of the standard BCA Protein Assay protocol (15-minute incubation with Compatibility Reagent); no precipitation steps required
  • Small samples – requires only 25µL (standard kit) or less than 10µL (microplate kit) of sample
  • Colorimetric – measure with a standard spectrophotometer or plate reader (562nm)
  • Excellent uniformity – exhibits less protein-to-protein variation than dye-binding methods
  • High linearity – linear working range for BSA equals 125 to 2000µg/mL

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