亚洲中文久久精品无码WW16,亚洲国产精品久久久久爰色欲,人人妻人人澡人人爽欧美一区九九,亚洲码欧美码一区二区三区

CUTANA pAG-Tn5 for CUT Tag 試劑返回列表頁(yè)

舉報(bào) 評(píng)價(jià)

參考價(jià):9540

具體成交價(jià)以合同協(xié)議為準(zhǔn)

型號(hào)
品牌
EpiCypher
廠商性質(zhì)
代理商
所在地
深圳市

規(guī)格

50 Reactions 9540元 59件可售

在線詢價(jià) 加入對(duì)比查看聯(lián)系電話

更新時(shí)間：2024-05-22 15:04:58瀏覽次數(shù)：369次

聯(lián)系我時(shí)，請(qǐng)告知來(lái)自化工儀器網(wǎng)

產(chǎn)品分類品牌分類

表觀遺傳學(xué)

全部產(chǎn)品列表

產(chǎn)品簡(jiǎn)介

CAS	請(qǐng)?jiān)?/td>	純度	請(qǐng)?jiān)?/td>
分子量	請(qǐng)?jiān)?/td>	分子式	請(qǐng)?jiān)?/td>
供貨周期	兩周	規(guī)格	50 Reactions
貨號(hào)	15-1017	應(yīng)用領(lǐng)域	生物產(chǎn)業(yè),綜合
主要用途	科研

詳情介紹

CUTANA pAG-Tn5是靶向剪切及轉(zhuǎn)座酶(CUT&Tag)技術(shù)中進(jìn)行高效繪制染色質(zhì)特征的關(guān)鍵試劑。與ChIP-seq相比，CUT&Tag在降低細(xì)胞需求量和測(cè)序深度的信噪比方面進(jìn)行了顯著改進(jìn)。CUTANA pAG-Tn5是一種高活性的E. coli轉(zhuǎn)座酶突變體(Tn5)與蛋白A/G的融合產(chǎn)物，可與來(lái)自多種物種宿主的靶抗體兼容。它也沒(méi)有表位標(biāo)簽，使其適合于標(biāo)簽介導(dǎo)的CUT&Tag(如FLAG、HA、TY1、V5等)。該產(chǎn)品經(jīng)過(guò)高度純化，已去除污染的E. coli DNA，并在細(xì)胞數(shù)量少的情況下進(jìn)行復(fù)雜分析。為了每次實(shí)驗(yàn)的標(biāo)準(zhǔn)化以及抗體驗(yàn)證和反應(yīng)監(jiān)測(cè)，推薦使用SNAP-CUTANA™ nucleosome spike-ins(如EpiCypher 19-1002)。Tn5帶有mosaic 接頭，可在CUT&Tag中使用。

Adapters

Tn5ME-A: 5’-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG-3’

Tn5ME-B: 5’-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG-3’

Tn5ME-rev: 5’-[phos]CTGTCTCTTATACACATCT-3’

組分

50 mM HEPES-KOH pH 7.2, 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1% Triton X-100, 50% glycerol.

保存條件

Stable for one year at -20℃ from date of receipt. The protein is not subject to freeze/thaw under these conditions.

數(shù)據(jù)示例

CUTANA pAG-Tn5 for CUT Tag 試劑

FIGURE 1:

Protein gel data. CUTANA pAG-Tn5 for CUT&Tag (1 µg) was resolved via SDS-PAGE and stained with Coomassie blue. The migration and molecular weight of the protein standards are indicated. Uncharged pAG-Tn5 monomer is 78.5 kDa, however once charged with DNA, Tn5 dimerizes to a final complex weight of 191 kDa.

CUTANA pAG-Tn5 for CUT Tag 試劑

FIGURE 2:

Size distribution of released chromatin. CUT&Tag was performed as described above. Recovered DNA was directly PCR amplified to produce sequenceready libraries. Agilent TapeStation traces for libraries derived from negative control IgG (top) and H3K27me3 (bottom) antibodies are shown. Excised DNA is highly enriched for mononucleosomes (peak at ~300 bp reflects ~150 bp insert size).

CUTANA pAG-Tn5 for CUT Tag 試劑

FIGURE 3:

CUT&Tag data. CUT&Tag was performed as described above. Representative sequencing tracks obtained using CUTANA pAG-Tn5 show a 229 kb close up view of the LAMC3 gene. CUTANA pAG-Tn5 produced clear peaks with genomic distribution profiles consistent with the known biological functions of H3K4me3 and H3K27me3 as well as minimal background in the IgG negative control.

訂購(gòu)詳情

貨號(hào)	產(chǎn)品名稱	規(guī)格
15-1017	CUTANA™ pAG-Tn5 for CUT&Tag	50 Reactions
15-1117	CUTANA™ pAG-Tn5 for CUT&Tag	250 Reactions