詳細介紹
質粒提取磁珠試劑盒
Mini Plasmid Kit采用超順磁性磁珠和優化的緩沖液體系,可方便、快捷地從大腸桿菌中提取超螺旋質粒。提取過程采用磁性分離,菌體裂解后不需要通過高速離心去除雜質,有利于實現高通量操作。提取的質粒可用于酶切、測序、PCR等后續操作。
產品信息
- 操作簡單:菌體裂解后無需離心或抽濾;
- 輕松實現高通量:適用于在96孔板中操作;
- 產物純度高:A 260/280=1.7~2.0。
應用實例
Agarose gel analysis of plasmids. Plasmid 1~Plasmid 4 were propagated in DH5α. Plasmid 5~Plasmid 7 were propagated in BL21(DE3) pLysS, *0 and JM109, respectively. M: Thermo Scientific GeneRuler 1kb DNA Ladder (# SM0311) A: Plasmid extracted by BeaverBeadsTM Mini Plasmid Kit (without centrifugation) B: Plasmid extracted by BeaverBeadsTM after centrifugation C: Plasmid extracted by spin column after centrifugation purifie |
如上圖所示,采用本產品提取的質粒DNA,純度及超螺旋的構象都能達到傳統競爭產品的水平,由于提取過程中采用磁珠法去除裂解液中的雜質(A),質粒DNA得率會略低于離心后再提取的樣品(B、C),但整個提取過程避免了高速離心步驟,有利于實現高通量及自動化操作。 |