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翌圣生物科技（上海）股份有限公司
初級會員·12年
- 聯系人：
- 曹女士
- 電話：
- 400-6111-883
- 手機：
- 售后：
- 4006-111-883
- 傳真：
- 86-21-34615995
- 地址：
- 上海市浦東新區天雄路166弄1號3樓
- 網址：
- www.yeasen.com
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產品簡介
| 供貨周期 | 現貨 | 應用領域 | 醫療衛生,生物產業,制藥 |
|---|
產品介紹
This enzyme is responsible for the first step in ubiquitin-protein isopeptide bond formation, by forming a high-energy thioester bond with ubiquitin. The ubiquitin is activated by first adenylating with ATP its Cterminal glycine residue (Gly76) and thereafter linking this residue to the side chains of a cysteine residue in UBE1, yielding an ubiquitin-UBE1 thiolester and free AMP and PPi. The activated ubiquitin is then transferred to a lysine of the targeted protein via the E2-E3 conjugation cascade. UBE1 is a critical component for the initiation of any in vitro conjugation reactions.
產品性質
別名(Synonyms) | UBA1, UBA6 |
來源(Source) | E.coli |
分子量 (Molecular Weight) | Approximately 118 kDa. |
氨基酸序列 (AA Sequence) | MSSSPLSKKRRVSGPDPKPGSNCSPAQSVLSEVPSVPTNGMAKNGSEADIDEGLYSRQLYVLGHEAMKRLQTSSVLVSGLRGLGVEIAKNIILGGVKAVTLHDQGTAQWADLSSQFYLREEDIGKNRAEVSQPRLAELNSYVPVTAYTGPLVEDFLSGFQVVVLPLEDQLRVGEFCHNRGIKLVVADTRGLFGQLFCDFGEEMILTDSNGEQPLSAMVSMVTKDNPGVVTCLDEARHGFESGDFVSFSEVQGMVELNGNQPMEIKVLGPYTFSICDT SNFSDYIRGGIVSQVKVPKKISFKSLVASLAEPDFVVTDFAKFSRPAQLHIGFQALHQFCAQHGRPPRPRNEEDAAELVALAQAVNARALPAVQQNNLDEDLIRKLAYVAAGDLAPINAFIGGLAAQEVMKACSGKFMPIMQWLYFDALECLPEDKEVLTEDKCLQRQNRYDGQVAVFGSDLQEKLGKQKYFLVGAGAIGCELLKNFAMIGLGCGEGGEIIVTDMDTIEKSNLNRQFLFRPWDVTKLKSDTAAAAVRQMNPHIRVTSHQNRVGPDTERIY DDDFFQNLDGVANALDNVDARMYMDRRCVYYRKPLLESGTLGTKGNVQVVIPFLTESYSSSQDPPEKSIPICTLKNFPNAIEHTLQWARDEFEGLFKQPAENVNQYLTDPKFVERTLRLAGTQPLEVLEAVQRSLVLQRPQTWADCVTWACHHWHTQYSNNIRQLLHNFPPDQLTSSGAPFWSGPKRCPHPLTFDVNNPLHLDYVMAAANLFAQTYGLTGSQDRAAVATFLQSVQVPEFTPKSGVKIHVSDQELQSANASVDDSRLEELKATLPSPDKLP GFKMYPIDFEKDDDSNFHMDFIVAASNLRAENYDIPSADRHKSKLIAGKIIPAIATTTAAVVGLVCLELYKVVQGHRQLDSYKNGFLNLALPFFGFSEPLAAPRHQYYNQEWTLWDRFEVQGLQPNGEEMTLKQFLDYFKTEHKLEITMLSQGVSMLYSFFMPAAKLKERLDQPMTEIVSRVSKRKLGRHVRALVLELCCNDESGEDVEVPYVRYTIR
|
外觀 | Liquid |
標簽(Tag) | None |
純度(Purity) | > 95% by SDS-PAG |
緩沖液(Buffer) | 50mM PBS, pH7.0, 150mM NaCl, 1mM DTT. |
儲存條件
-25~-15℃保存
注意事項
1. 避免反復凍融。
2. 本產品僅作科研用途。
2. 為了您的安全和健康，請穿實驗服并佩戴一次性手套操作。
反應緩沖液配方
名稱 | 組分 | 濃度 |
10 × Reaction Buffer（200 ul） | Tris PH8.0 | 500 mM |
DTT | 6 mM | |
ddH2O | to 200μL | |
10 x ATP-Mg （200 ul）
| ATP | 100 mM |
MgCl2 | 100 mM | |
ddH2O | to 200μL |
參考陽性反應體系
組分 | 體積 (μL) |
ddH2O | to 20 μL |
10 × Reaction Buffer | 2 μL |
10 × E1 Enzyme | 2 μL |
5 × Ubiquitin | 4 μL |
10 × ATP-Mg | 2 μL |
20 × E2 Enzyme | 1 μL |
10 × E3 Ligase Itch (Positive control) | 2 μL |
20 × S5a substrate (Positive control) | 1 μL |
