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- 聯系人:
- 曹女士
- 電話:
- 400-6111-883
- 手機:
- 售后:
- 4006-111-883
- 傳真:
- 86-21-34615995
- 地址:
- 上海市浦東新區天雄路166弄1號3樓
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- www.yeasen.com
掃一掃訪問手機商鋪
供貨周期 | 現貨 | 貨號 | 93968ES60 |
---|---|---|---|
應用領域 | 醫療衛生,生物產業,制藥 |
性能參數
表達區間及表達系統(Source) | Biotinylated Human IGF1R/CD221 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Glu31-Asn932.[Accession | P08069] |
分子量大小(Molecular Weight) | The protein has a predicted MW of 105.8 kDa (alpha subunit) and 23 kDa (beta subunit). Due to glycosylation, the protein migrates to 110-120 kDa (alpha subunit) and 52-55 kDa (beta subunit) based on Tris-Bis PAGE result. |
內毒素(Endotoxin) | <1EU per μg by the LAL method. |
純度(Purity) | > 95% as determined by Tris-Bis PAGE and HPLC |
活性(Activity) | ELISA Data: Immobilized Anti-IGF1R Antibody at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IGF1R, His Tag with the EC50 of 48.0ng/ml determined by ELISA. ELISA Data:Immobilized Biotinylated Human IGF1R, His Tag at 5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human IGF-I, hFc Tag with the EC50 of 0.73μg/ml determined by ELISA. |
制劑(Formulation) | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
重構方法(Reconstitution) | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
儲存條件
The product should be stored at -25~-15℃ for 1 year from date of receipt.
2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.
3 months, -25~-15℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
注意事項
1.Please operate with lab coats and disposable gloves,for your safety.
2.This product is for research use only.
Ver. CN20230721