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豬繁殖與呼吸綜合征病毒抗體檢測試劑盒

名稱和用途

Prionics 藍耳病 豬繁殖與呼吸綜合征抗體檢測試劑盒是用于檢測豬血清和血漿中的豬繁殖與呼吸綜合征病毒(PRRSV)抗體的酶聯免疫檢測試劑盒。

概述

豬繁殖與呼吸綜合征病毒（PRRSV）是一種動脈炎病毒，在80 年代后期*在歐洲和美國發現，兩種毒株具有不同的抗原性。該病毒引起繁殖問題（ 流產，產弱仔，死胎， 母豬死亡）、呼吸系統疾病（肺炎，呼吸困難，行動遲緩，死亡）和輕度神經癥狀1。豬繁殖與呼吸綜合征引起嚴重的經濟損失，被認為是影響養豬業zui重要的疾病之一。可以通過便捷的檢測血清或血漿中的抗體評估豬只是否感染PRRSV（無論是自然感染或人工免疫疫苗）。需要注意的是這種檢測抗體的免疫學方法需要對不同的PRRS 毒株的檢測都很敏感。

此試劑盒僅供體外檢測使用。

原理

Prionics試劑盒通過*的抗體設計，能同時檢測美洲株和歐洲株不同血清型的藍耳病毒。將重組的ORF7蛋白 （美洲株和歐洲株）包被在微孔滴定板上，被測樣品在包被孔中孵育時，針對PRRSV的特異性抗體與包被的抗原形成抗原抗體復合物，洗去包被孔中沒有結合的成分后，加入辣根過氧化物酶（HRPO）標記的抗豬抗體，它與孔中的豬抗體結合，在試驗的zui后步驟，洗去沒有結合的酶標抗體，再加入酶底物和顯色劑TMB，顏色反應的深淺由樣品中存在的抗PRRSV 特異性抗體的量決定。

The PrioCHECK? PRRSV Antibody ELISA is specially designed for the detection of both the American and the European genotypes of the PRRS virus in serum samples. Plates are coated with ORF 7 protein (common to both genotypes) ensuring the detection of antibodies to both virus types with high sensitivity and specificity. The PrioCHECK? PRRSV Antibody ELISA shows a high agreement with the most frequently used ELISA in the field and is therefore the most powerful alternative on the PRRS test market.

Test procedure
The PrioCHECK? PRRSV Antibody ELISA is a diagnostic kit based on ELISA technology. It follows a simple four step protocol consisting of sample preparation, sample incubation, conjugate incubation, and detection. Easy handling makes the test convenient and fast: 90 samples can be analyzed within 150 minutes. Serum samples are incubated in an ELISA plate coated with the ORF 7 protein of PRRSV. Anti-PRRSV antibodies present in the sample bind to the antigen. Plates are then washed and an anti-swine antibody labeled with horse radish peroxidase (HRPO) is used to detect antibodies bound to the ORF 7 coated plate. Color development using tetramethyl-benzidine (TMB) substrate measured optically at a wavelength of 450 nm shows the presence of antibodies directed against PRRSV.