廣州測迪生物科技有限公司
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廣州測迪生物科技有限公司
主營產品: 美國kernel試劑盒,德國拜發試劑盒,瑞士Prionics試劑盒,荷蘭賽迪試劑盒 |
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更新時間:2018-04-17 02:16:12瀏覽次數:965
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豬瘟病毒抗體檢測試劑盒
Classical Swine Fever Virus Antibody Test Kit (CSFV Ab)
概述
豬瘟病毒(CSFV)、牛病毒性腹瀉性病毒(BVDV)以及邊界病(BDV)都屬于黃病毒屬的成員。由于CSFV的高致病力和高致死率,它已給養殖業造成了巨大的損失。豬感染高致病力毒株后在潛伏期就可以造成傳染,經歷急性豬瘟或亞急性豬瘟臨床癥狀的豬在出現癥狀前大量排毒,但耐過后的豬只體內有明顯的CSFV抗體,且不再排毒。感染溫和性毒株的豬呈慢性感染,在病死前會長期或間歇性地排毒。懷孕母豬可以通過胎盤感染胎兒導致流產、木乃伊化或產出弱豬、畸形胎等。先天性感染低毒力病毒的結果是產出持續性感染的仔豬,仔豬出現免疫耐受,不表現任何癥狀向外排毒。豬也有可能感染BVDV或BDV。盡管這些感染通常呈溫和過程并且是自限性的,但有效的將它們同豬瘟病毒區分是很重要的。
原理
豬瘟病毒抗體ELISA檢測試劑盒是用來檢測豬血清或血漿中豬瘟病毒抗體的檢測試劑盒。該試劑盒是用豬瘟病毒抗原包被的微量反應板,利用阻斷ELISA原理來檢測豬血清或血漿中豬瘟病毒抗體。如果被檢樣品中存在豬瘟病毒抗體,它們就會阻斷辣根過氧化物酶標記(HRPO)的抗豬瘟病毒的單克隆抗體。單克隆抗體與豬瘟病毒抗原的結合可以通過辣根過氧化物酶與底物的顯色程度進行判定,即用酶標儀在單波長450nm或雙波長450nm與620nm測定該反應體系的吸光度。當被檢樣品中含有豬瘟病毒抗體(陽性結果)時,顯色就會變淺,當被檢樣品中不含有抗豬瘟病毒抗體(陰性結果)時,顯色就會變深。樣本的阻斷率可以通過450nm波長樣本吸光度與陰性對照吸光度的比值來確定。
The PrioCHECK? CSFV Antibody 2.0 ELISA is a blocking ELISA that specifically detects antibodies against the A domain of the E2 protein of CSFV. This domain is more specific for CSFV than the B, C, and D domain of the E2 protein. An important advantage of the test is that it does not cross-react with related viruses such as Bovine Viral Diarrhoea virus (BVDV) and Border Disease virus (BDV). The test detects antibodies against high-, moderate-, and low-virulent CSFV strains early after infection. Detection of antibodies elicited by low-virulent CSFV strains is of paramount importance to trace sub clinical CSFV infections.
Test procedure
The PrioCHECK? CSFV Antibody 2.0 ELISA is a blocking ELISA. In this test, the plates are coated with the E2 CSFV envelope protein. The key reagent is a monoclonal antibody (mAb) directed against an epitope located on the A domain of the envelope protein E2 of CSFV, making the test more specific for CSFV. The mAb is labeled with an enzyme that generates a color signal. The signal is measured and when no color is formed, the sample containing anti-CSFV antibodies that competed for the A domain of the E2 protein is positive for CSFV.