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描述

Thermo Scientific Phire Animal Tissue Direct PCR Kit has been developed for amplification of DNA directly from a wide variety of animal tissues including mice, fish, birds and insects. The kit contains all the necessary components for amplification of DNA directly from animal tissues: optimized PCR reagents, Dilution buffer and DNARelease Additive, which can be used to improve the release of DNA from animal tissues. In addition, the kit includes control primers for positive control reactions that are universal and work with numerous animal species. A detailed manual is provided that describes protocols when working with different types of tissues.

Highlights

• No need for time-consuming and expensive DNA purification steps
• Very little sample material required
• Two simple protocols for various applications
• Phire Hot Start II DNA Polymerase provides high yields of specific product with short extension time (20 s/kb). 

The optimal annealing temperature for Phire DNA Polymerase may differ significantly from that of Taq-based polymerases.

For optimal results start by accurately calculating your Tm with our Tm calculator.

規(guī)格

Polymerase:	Phire Hot Start II DNA polymerase
Hot Start:	Built-In Hot Start
GC-Rich PCR Performance:	Medium
Reaction Format:	Separate Components
Product Overhang:	Blunt
Reaction Speed:	Fast
Product Line:	Phire
Product Size:	500 reactions

內(nèi)容及儲(chǔ)存

Contains: 
• 200 µL Phire Hot Start II DNA Polymerase 
• 5 x 1.25 mL 2X Phire Animal Tissue PCR Buffer (includes dNTPs and MgCl2) 
• 40 µL Control Primer mix (25 µM each) 
• 2 x 5 mL Dilution Buffer 
• 3 × 100 µL DNARelease Additive 
• 3 × 1 mL Gel loading dye 

Store at -20°C.