如果采用前述前處理方法對生物樣品進行處理后,依然發現目標組分受干擾比較嚴重,則建議采用更有效的前處理方法,就是固相萃取方法。固相萃取(Solid Phase Extraction, 簡稱SPE)是一種包括液相和固相的萃取過程,是通過填料的鍵合官能基團和待分離化合物之間的作用力,將目標化合物與樣品基質分離,以達到除去雜質和富集目標組分的目的,固相萃取小柱的種類較多,用于生物樣品凈化前處理的固相萃取填料主要有以下幾種:
■固相萃取填料類型和結構
■根據化合物類型選擇對應的固相萃取填料
■通用操作方法
固相萃取產品訂購信息
【EVOLUTE EXPRESS系列】
■聚合物基質反相填料—EVOLUTE EXPRESS ABN
96-Well PlatePN. | Description | Qty. |
---|
600-0010-PX01 | EVOLUTE Express ABN 10mg Plate | 1 |
600-0030-PX01 | EVOLUTE Express ABN 30mg Plate | 1 |
Tabless SPE ColumnPN. | Description | Qty. |
---|
600-0001-AXG | EVOLUTE Express ABN 10mg/1 mL | 100 |
600-0003-AXG | EVOLUTE Express ABN 30mg/1 mL | 100 |
610-0006-BXG | EVOLUTE Express ABN 60mg/3 mL | 50 |
610-0010-BXG | EVOLUTE Express ABN 100mg/3 mL | 50 |
610-0015-CXG | EVOLUTE Express ABN 150mg/6 mL | 30 |
610-0050-CXG | EVOLUTE Express ABN 500mg/6 mL | 30 |
■混合型強陽離子交換填料—EVOLUTE EXPRESS CX
96-Well PlatePN. | Description | Qty. |
---|
601-0010-PX01 | EVOLUTE Express CX 10mg Plate | 1 |
601-0030-PX01 | EVOLUTE Express CX 30mg Plate | 1 |
Tabless SPE ColumnPN. | Description | Qty. |
---|
601-0001-AXG | EVOLUTE Express CX 10mg/1 mL | 100 |
601-0003-AXG | EVOLUTE Express CX 30mg/1 mL | 100 |
611-0006-BXG | EVOLUTE Express CX 60mg/3 mL | 50 |
611-0010-BXG | EVOLUTE Express CX 100mg/3 mL | 50 |
611-0015-CXG | EVOLUTE Express CX 150mg/6 mL | 30 |
611-0050-CXG | EVOLUTE Express CX 500mg/6 mL | 30 |
■混合型強陰離子交換填料—EVOLUTE EXPRESS AX
96-Well PlatePN. | Description | Qty. |
---|
601-0010-PX01 | EVOLUTE Express CX 10mg Plate | 1 |
601-0030-PX01 | EVOLUTE Express CX 30mg Plate | 1 |
Tabless SPE ColumnPN. | Description | Qty. |
---|
601-0001-AXG | EVOLUTE Express CX 10mg/1 mL | 100 |
601-0003-AXG | EVOLUTE Express CX 30mg/1 mL | 100 |
611-0006-BXG | EVOLUTE Express CX 60mg/3 mL | 50 |
611-0010-BXG | EVOLUTE Express CX 100mg/3 mL | 50 |
611-0015-CXG | EVOLUTE Express CX 150mg/6 mL | 30 |
611-0050-CXG | EVOLUTE Express CX 500mg/6 mL | 30 |
■混合型強陰離子交換填料—EVOLUTE EXPRESS AX
96-Well PlatePN. | Description | Qty. |
---|
603-0010-PX01 | EVOLUTE Express AX 10mg Plate | 1 |
603-0030-PX01 | EVOLUTE Express AX 30mg Plate | 1 |
Tabless SPE ColumnPN. | Description | Qty. |
---|
603-0001-AXG | EVOLUTE Express AX 10mg/1 mL | 100 |
603-0003-AXG | EVOLUTE Express AX 30mg/1 mL | 100 |
613-0006-BXG | EVOLUTE Express AX 60mg/3 mL | 50 |
613-0010-BXG | EVOLUTE Express AX 100mg/3 mL | 50 |
613-0015-CXG | EVOLUTE Express AX 150mg/6 mL | 30 |
613-0050-CXG | EVOLUTE Express AX 500mg/6 mL | 30 |
■混合型弱陽離子交換填料—EVOLUTE EXPRESS WCX
96-Well PlatePN. | Description | Qty. |
---|
602-0010-PX01 | EVOLUTE Express WCX 10mg Plate | 1 |
602-0030-PX01 | EVOLUTE Express WCX 30mg Plate | 1 |
Tabless SPE ColumnPN. | Description | Qty. |
---|
602-0001-AXG | EVOLUTE Express WCX 10mg/1 mL | 100 |
602-0003-AXG | EVOLUTE Express WCX 30mg/1 mL | 100 |
612-0006-BXG | EVOLUTE Express WCX 60mg/3 mL | 50 |
612-0010-BXG | EVOLUTE Express WCX 100mg/3 mL | 50 |
612-0015-CXG | EVOLUTE Express WCX 150mg/6 mL | 30 |
612-0050-CXG | EVOLUTE Express WCX 500mg/6 mL | 30 |
■混合型弱陰離子交換填料—EVOLUTE EXPRESS WAX
96-Well PlatePN. | Description | Qty. |
---|
604-0010-PX01 | EVOLUTE Express WAX 10mg Plate | 1 |
604-0030-PX01 | EVOLUTE Express WAX 30mg Plate | 1 |
Tabless SPE ColumnPN. | Description | Qty. |
---|
604-0001-AXG | EVOLUTE Express WAX 10mg/1 mL | 100 |
604-0003-AXG | EVOLUTE Express WAX 30mg/1 mL | 100 |
614-0006-BXG | EVOLUTE Express WAX 60mg/3 mL | 50 |
614-0010-BXG | EVOLUTE Express WAX 100mg/3 mL | 50 |
614-0015-CXG | EVOLUTE Express WAX 150mg/6 mL | 30 |
614-0050-CXG | EVOLUTE Express WAX 500mg/6 mL | 30 |
■EVOLUTE EXPRESS 系列方法開發板
EVOLUTE EXPRESS 系列方法開發板包含四種混合模式的填料, EVOLUTE EXPRESS CX,WCX, AX和WAXPN. | Description | Qty. |
---|
650-0010-PX01 | EVOLUTE Express Sorbent Selection Plate 10mg | 1 |
650-0030-PX01 | EVOLUTE Express Sorbent Selection Plate 30mg | 1 |
【Panthera 和Agility系列 】
■聚合物基質反相填料-Panthera
96-Well PlatePN. | Description | Qty. |
---|
OMNPA6030 | Panthera Deluxe,Polymer DVB 96-well plate,30mg/1mL | 1 |
OC21PA6030 | Panthera Deluxe,Polymer DVB 96-well plate,30mg/2mL | 1 |
SPE ColumnsPN. | Description | Qty. |
---|
SYPA6030-01RL | Panthera Deluxe, 30mg/1cc rimless long luer cartridges | 1 |
■混合型強陽離子交換填料—Agility SCX
96-Well PlatePN. | Description | Qty. |
---|
OMNAG2030 | Agility SCX, Polymer DVB 96-well plate, 30 mg/1 mL | 1 |
OC21AG2030 | Agility SCX, Polymer DVB 96-well plate, 30 mg/2 mL | 1 |
OMNAD2030 | Agility Deluxe SCX, Polymer DVB 96-well plate, 30 mg/1 mL | 1 |
OC21AD2030 | Agility Deluxe SCX, Polymer DVB 96-well plate, 30 mg/2 mL | 1 |
SPE ColumnsPN. | Description | Qty. |
---|
SYAG2025-01RL | Agility SCX, 30mg/1cc rimless long luer cartridges | 100 |
SYAD2030-01RL | Agility Deluxe SCX, 30mg/1cc rimless long luer cartridges | 100 |
■混合型強陰離子交換填料—Agility SAX
96-Well PlatePN. | Description | Qty. |
---|
OMNAG5030 | Agility SAX, Polymer DVB 96-well plate, 30 mg/1 mL | 1 |
OC21AG5030 | Agility SAX, Polymer DVB 96-well plate, 30 mg/2 mL | 1 |
SPE ColumnsPN. | Description | Qty. |
---|
SYAG5025-01RL | Agility SAX, 30mg/1cc rimless long luer cartridges | 100 |
■混合型弱陽離子交換填料—Agility WCX
96-Well PlatePN. | Description | Qty. |
---|
OMNAG8030 | Agility WCX, Polymer DVB 96-well plate, 30 mg/1 mL | 1 |
OC21AG8030 | Agility WCX, Polymer DVB 96-well plate, 30 mg/2 mL | 1 |
SPE ColumnsPN. | Description | Qty. |
---|
SYAG8025-01RL | Agility WCX, 30mg/1cc rimless long luer cartridges | 100 |
■混合型弱陰離子交換填料—Agility WAX
96-Well PlatePN. | Description | Qty. |
---|
OMNAD7030 | Agility Deluxe WAX, Polymer DVB 96-well plate, 30 mg/1 mL | 1 |
OC21AD7030 | Agility Deluxe WAX, Polymer DVB 96-well plate, 30 mg/2 mL | 1 |
SPE ColumnsPN. | Description | Qty. |
---|
SYAD7030-01RL | Agility Deluxe WAX, 30mg/1cc rimless long luer cartridges | 100 |
■96孔板底座——可支持 SPE Columns
PN. | Description | Qty. |
---|
ORO1MLBLK | 1mL 96-Well holder block | 100 |