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Bovine blood from healthy calves 2 - 10 days old which have been inspected ante and post mortem by Australian or New Zealand Government veterinarians and passed as fit for human consumption.

COLLECTION/ PROCESSING METHOD:

Blood is collected into sanitary containers, defibrinated and centrifuged under temperature controlled conditions. Serum is subsequently passed through a series of filters terminating with 0.2 micron pharmaceutical grade filters. Product is dispensed in Class 100 Laminar Flow Workstation using aseptic techniques. It is then immediay frozen.

DESCRIPTION:

A clear amber viscous liquid with a characteristic odour.

TEST	METHOD	SPECIFICATION
Visual Examination	Visual	Satisfactory
Specificity	Species I.D.	Bovine
Sterility	Millipore Steritest Broth Culture 14 Day Incubation	Sterile
Mycoplasma	Broth Culture 28 Day Incubation	Not Detected
Viruses: Modified 9CFR
Bovine Viral Diarrhea Virus （BVDV）	Fluorescent Antibody	Not Detected
Infectious Bovine Rhinotracheitis	Cytopathic Agents	Not Detected
Para Influenza 3 （PI3）	Haemadsorbing Agent	Not Detected
Antibodies:
BVD	VNT	As Reported
IBR	VNT	As Reported
PI3	Elisa	As Reported
pH	pH Meter	6.9 - 7.9
Osmolality:	Osmometer	240 - 340 mOsmol/kg
Total Protein	COBAS Fara Analyser	> 35mg/ml
Albumin	Electrophoresis	As Reported g/l
Total Globulins	Electrophoresis	As Reported g/l
Alpha 1	Electrophoresis	As Reported g/l
Alpha 2	Electrophoresis	As Reported g/l
Beta	Electrophoresis	As Reported g/l
Gamma	Electrophoresis	As Reported g/l
Electrophoresis pattern	Agarose Gel Plate	Typical
Haemoglobin	UV/VIS Spectrophotometer	< 0.2mg/ml
Endotoxin	LAL	<10 IU/ml

FUNCTIONAL TEST:

MDBK	Cell Culture	>=85% of FCS Control
Hybridoma	Cell Culture	>=85% of FCS Control
MRC-5	Cell Culture	>=85% of FCS Control
Vero	Cell Culture	>=85% of FCS Control

EXPIRY DATE:

Six years from the date of manufacture

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