Efficient, gentle lysis and extraction of E.coli and other bacterial cells.

B-PER Bacterial Protein Extraction Reagents are designed to extract soluble protein from bacterial cells. Reagent based assay to eliminate the need for harsh mechanical procedures like sonication. These easy-to-use cell lysis solution use mild nonionic detergents to disrupt cells and solubilize proteins without denatur-ation. Enzyme supplements such as DNase I and Lysozyme allow for improved yield of large molecular weight proteins which are difficult to purify. B-PER is also compatible with downstream protein purification methods such as GST purification and His-tagged immobilized metal affinity chromatography （IMAC）.

The first step to purify or characterize a recombinant protein is to disrupt the cell and release the protein. Sonication and other cell lysis methods are not as effective as the B-PER Solutions, and typical homemade lysis buffers include detergents and components that interfere with downstream applications. The B-PER Bacterial Protein Extraction Reagents are formulated in 20 mM Tris buffer （pH 7.5） or phosphate buffer （Product # 78266）, yielding lysates that are directly compatible with typical downstream workflows such as electrophoresis, affinity purification, immunoprecipitation, protein interaction analysis, crosslinking and protein labeling. If necessary, the mild detergent components can be removed by dialysis or gel filtration （desalting columns）.

B-PER Bacterial Protein Extraction Reagent Highlights:

• Fast and simple – just add B-PER Reagent to a bacterial pellet and shake for 10 minutes.
  • Obtain clarified soluble protein extract by pelleting cell debris
  • Isolate inclusion bodies from the pellet using an optimized procedure
• Recover soluble and insoluble recombinant protein – purify inclusion bodies to homogeneity
• Flexible – B-PER Reagents are suitable for any scale of protein extraction
• Compatible – compley compatible with addition of protease inhibitor cocktails, and resulting protein extract can be used in protein assays, typical affinity purification methods （e.g., GST, 6xHis） and other applications
• Convenient, ready-to-use formats:
  • B-PER and B-PER II （2X） are free of enzymatic components
  • B-PER （in Phosphate Buffer） is an amine-free formulation for direct compatibility with labeling and crosslinking
  • B-PER with Enzymes and B-PER Direct with Enzymes include lysozyme and DNase enzymes for improved lysis and recovery

Efficient and complete protein extraction with Thermo Scientific B-PER Reagent. E. Coli expressing green fluorescent protein （GFP） was extracted five times with B-PER Reagent or PBS/sonication. Equal amounts of each extract was by separated by SDS-PAGE and stained with coomassie dye. Extraction with B-PER Reagent was nearly complete after three rounds （Lanes 1-3）. Analysis of GFP activity （below） confirms that nearly all GFP protein was extracted in the first three rounds with B-PER Reagent.

Related Products
I-PER Insect Cell Protein Extraction Reagent
Y-PER Yeast Protein Extraction Reagent
Inclusion Body Solubilzation Reagent
B-PER 6xHis Fusion Protein Spin Purification Kit
B-PER 6xHis Fusion Protein Purification Kit
B-PER GST Fusion Protein Purification Kits
Halt Protease Inhibitor Cocktail
Lysozyme
DNase I

Related Links
Poppers Cell Lysis Handbook
Remove detergent from protein samples
Cell Lysis Technical Information