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重組小鼠白介素-2(rmIL-2)

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CYT-370重組小鼠白介素-2(rmIL-2) 5μg/20μg/1mg Prospec

 

 

Description :
 Recombinant Murine IL-2 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 149 amino acids and having a molecular mass of 17231 Dalton.
The Recombinant Murine IL-2 is purified by proprietary chromatographic techniques.
 
Physical Appearance:
 Sterile Filtered White lyophilized (freeze-dried) powder.
 
Formulation:
 The protein (1mg/ml) was lyophilized after extensive dialysis against 10mM sodium citrate buffer to a pH=4.
 
Solubility:
 It is recommended to reconstitute the lyophilized Murine IL-2 in sterile 20mM AcOH not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
 
Stability:
 Lyophilized Recombinant Murine IL-2 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution Murine IL-2 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.
 
Purity:
 Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
 
Amino acid sequence:
 The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Thr-Ser-Ser.
 
Dimers and aggregates:
 Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
 
Biological Activity:
 ProSpec’s Recombinant Murine IL-2 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of murine CTLL-2 cells is < 0.2 ng/ml, corresponding to a Specific Activity of 5 MIU/mg.
 
Endotoxin:
 Less than 0.1 ng/µg (IEU/µg) of Murine IL-2.
 
Protein content:
 Protein quantitation was carried out by two independent methods:

1. UV spectroscopy at 280 nm using the absorbency value of 0.482 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (InliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of IL-2 as a Reference Standard.
 
Usage:
 Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

 


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