當前位置:上海易佰聚經貿有限公司>>以色列Prospec細胞因子>> 重組人紅細胞生成素-α(rHuEPO-a)
重組人血小板生成素TPO/Thrombopoietin CHO
CYT-201 | 重組人紅細胞生成素-α(rHuEPO-a) | 5ug/50ug | Prospec |
Description : | |
Recombinant Human Erythropoietin-alpha is produced in Chinese hamster ovary (CHO) cells by recombinant DNA technology is a single, polypeptide chain containing 165 amino acids and having a predicted molecular mass of 21,000 Dalton and apparent glycosylated molecular mass of 30,400 Dalton. EPO-alpha is purified by proprietary chromatographic techniques. | |
Physical Appearance: | |
Sterile Filtered White lyophilized (freeze-dried) powder. | |
Formulation: | |
Recombinant Erythropoietin-alpha was lyophilized after extensive dialysis in 5.8mg sodium citrate, 5.8mg sodium chloride and 0.06mg citric acid. | |
Solubility: | |
It is recommended to reconstitute the lyophilized EPO-a in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | |
Stability: | |
Lyophilized Epoetin Alfa although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution EPO should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles. | |
Purity: | |
Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. | |
Dimers and aggregates: | |
Less than 1% as determined by silver-stained SDS-PAGE gel analysis. | |
Biological Activity: | |
ProSpec's Erythropoietin is fully biologically active when compared to standard. The Specific Activity was measured by Normocyth-aemic mice and was found to be 120,000IU/mg. | |
Endotoxin: | |
Less than 0.1 ng/µg (IEU/µg) of Epoetin Alfa . | |
Usage: | |
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
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