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纖維粘連蛋白(FN)存在于間隙基質(zhì)和血漿中,通過RGD序列介導(dǎo)細(xì)胞的粘附。FN的主要功能是在傷口愈合過程中促進(jìn)細(xì)胞的遷移。有FN包被的細(xì)胞培養(yǎng)表面,對細(xì)胞的粘附,伸展和增殖有促進(jìn)作用。FN也可以用作無血清培養(yǎng)基的添加物。
產(chǎn)品描述 | 數(shù)量 | 貨號 |
BDTM 纖維粘連蛋白,人 BDTM Fibronectin, human | 1 mg | 354008 |
5 mg | 356008 |
來源:人血漿
分子量:440 kD
使用指南:根據(jù)細(xì)胞類型不同,推薦工作濃度為1-5 μg/cm2生長面積或5 ug/ml培養(yǎng)添加劑。
保存條件和效期:凍干粉2-8℃下保存或溶解后-20℃下保存,避免多次凍融。保質(zhì)期詳見產(chǎn)品包裝。
Fibronectin, human, used as a thin coating on tissue-culture surfaces to promote attachment, spreading, and proliferation of a variety of cell types.
Purity ≥ 90% by SDS-PAGE
Tested for ability to promote attachment and spreading of BHK-21 cells
Filtered (0.2 µm membrane) and tested and found negative for bacteria, fungi, and mycoplasma
Human plasma. NOTE: Source material tested for hepatitis B antigen and HIV-1 antibody.
440 kD (unreduced form)
Stable for three months at 2° to 8°C (lyophilized) or two weeks at -20°C (solubilized). Do not store in frost-free freezer. Avoid multiple freeze-thaws
Instructions for Use
Recommended concentration - 1-5 µg/cm2 of growth surface for coating or at 5 µg/ml as a media additive (guidelines for coating included with product).
Lyophilized from CAPS buffer containing NaCl and CaCl2, pH 11.0; reconstitute in dH20 (do not agitate or swirl).
Catalog Number
354008
Brand
BD™
Name
Fibronectin, human, 1 mg
Size
1 mg
Regulatory Status
RUO
Type Full:
Fibronectin, human
Quantity Pack:
0 type :
Quantity Case:
0
Packaging:
1 mg
Shipping:
Refrigerated shipping WI
Storage:
Storage: 2-8°C (lyophilized) or two weeks at -20°C (solubilized); Shelf life: >= 3 months from date of shipment
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