FuGENE  HD轉(zhuǎn)染試劑

*30%的轉(zhuǎn)染用戶使用的FuGENE®，其優(yōu)勢在于：

· Off-target效應(yīng)低 – 轉(zhuǎn)染試劑將外源核酸片段導(dǎo)入細胞的同時，也將影響內(nèi)源基因表達水平，從而對細胞的代謝和狀態(tài)造成不利影響，或影響實驗數(shù)據(jù)的客觀性，這稱為off-target效應(yīng)。FuGENE®只是LipofectAMINE 2000的16%，試驗表明，轉(zhuǎn)染MCF-7 cells （ATCC® HTB-22™）時，采用micro-array檢查發(fā)現(xiàn)，使用LipofectAMINE 2000轉(zhuǎn)染導(dǎo)致細胞內(nèi)源1741個基因表達發(fā)生變化，而使用FuGENE® HD只有282個內(nèi)源基因表達發(fā)生變化。[發(fā)表于Biochemica 4, 22-25 （2006），請登www.roche-applied-science.com/publications/biochemica.htm閱讀電子版]

· 細胞毒性低 – 這是下游實驗順利進行的保證

FuGENE® HD轉(zhuǎn)染試劑

繼堪稱轉(zhuǎn)染試劑精粹的FuGENE® 6之后，羅氏推出全新的FuGENE® HD，全面提升轉(zhuǎn)染實驗結(jié)果，為您實驗助一臂之力。

· 轉(zhuǎn)染目前已有的轉(zhuǎn)染試劑難轉(zhuǎn)染的細胞株，例如：MCF-7，RAW 267.7，PC-3，Hela，MA-10，HepG2，SH-SY5Y，A7r5，STO，SCC-61，STSAR-90，SQ20B，T98G，STSAR-90，A375，A549等；

· 同時適用于昆蟲細胞，多種癌癥細胞株，原代細胞和干細胞轉(zhuǎn)染；

· 更低的細胞毒性，有助獲得更多具有活性的功能細胞，避免和減少使用其他轉(zhuǎn)染試劑導(dǎo)致的對細胞應(yīng)答和細胞基因表達的影響；

· 顯著提升目的基因在常用的細胞株中的蛋白表達水平，通過調(diào)整轉(zhuǎn)染試劑的用量，就可輕松優(yōu)化蛋白表達；

· 更安全可靠的組份，不含任何動物來源成份，經(jīng)0.1um過濾滅菌，100%血清中依然有活性；

· 穩(wěn)定的試劑，室溫狀態(tài)下依然穩(wěn)定；簡便的操作步驟，快速實驗方案同時適用多種細胞，滿足大通量的實驗操作需要。

ROCHE相關(guān)產(chǎn)品資料

FuGENE^® HD Transfection Reagent

FuGENE^® HD Transfection Reagent is a multi-component reagent that forms a complex with DNA , then transports the complex into animal or insect cells.

Application

FuGENE^® HD Transfection Reagent is a next-generation transfection reagent, free of animal-derived components. It is designed to transfect a wide range of eukaryotic cells including insect cells and many cell lines not transfected well by other reagents （e.g .,  MCF-7, RAW 264.7, PC-3, HeLa, MA-10, HepG2, SH-SY5Y, A7r5, STO, SCC-61, STSAR-90, SQ20B, T98）.

Note: For the most up-to-date list of cell types that have been successfully transfected with FuGENE^® HD Transfection Reagent, visit our Transfection Special Interest Site at www.powerful-transfection.com and view our Transfection Reference Database. This site also contains much more information about the use of this reagent.

For life science research only.

Product Description

Composition: unique and proprietary blend of many components,  in 80% ethanol.

Storage:  stable at +2°C to +8°C.

Stability: 2 years, starting from date of manufacture.

Contents

Solution in 80% ethanol, filter-sterilized （0.1 µm filter）, packaged in transparent glass vials.

Quality

Lot-specific analysis: 
Each lot of FuGENE^®HD Transfection Reagent is carefully checked, using ISO DIN 9001-certified procedures, to assure product is performing according to specifications and is consistent from lot to lot.

Functional analysis:
Cos 7 cells are transfected in 96 well plates with a reporter gene vector DNA, using 0.3 µl FuGENE^® HD and 0.1 µg DNA or 0.25 µl FuGENE^® HD and 0.1 µg DNA. Reporter gene activity is monitored via a colorimetric test against a reference lot. Activity must be ≥ 75% compared to a reference lot in at least one of the tested amounts, listed above.

Cytotoxicity analysis:
Cell growth is assessed using the Cell Proliferation Reagent WST-1assay  which is an indirect measure of cytotoxicity. The remainder of cells from functional analysis is tested for viability.