Rabbit Tumor necrosis factor α(TNF-α) ELISA Kit
兔腫瘤壞死因子α(TNFα)ELISA試劑盒實驗原理 ：
本試劑盒用于測定兔血清，血漿及相關(guān)液體樣本中腫瘤壞死因子α（TNF-α）的含量。應(yīng)用雙抗體夾心法測定標(biāo)本中兔腫瘤壞死因子α（TNF-α）水平。用純化的兔腫瘤壞死因子α（TNF-α）抗體包被微孔板，制成固相抗體，往包被單抗的微孔中依次加入腫瘤壞死因子α，再與 HRP 標(biāo)記的羊抗兔抗體結(jié)合，形成抗體-抗原-酶標(biāo)抗體復(fù)合物，經(jīng)過*洗滌后加底物 TMB 顯色。TMB 在 HRP 酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成zui終的黃色。顏色的深淺和樣品中的兔腫瘤壞死因子α呈正相關(guān)。用酶標(biāo)儀在 450nm 波長下測定吸光度（OD 值），通過標(biāo)準(zhǔn)曲線計算樣品中兔腫瘤壞死因子α（TNF-α）濃度。
Principle of the assay:
This kit allows for the determination of TNF-α concentrations in Rabbit serum, blood plasma, and other biological fluids.The kit assay Rabbit TNF-α level in the sample，use Purified Rabbit TNF-α antibody to
coat microtiter plate wells, make solid-phase antibody, then add TNF-α to wells, Combined antibody which With HRP labeled goat anti-rabbit become antibody - antigen -enzyme-antibody complex, after washing Compley, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TNF-α in the samples is then determined by comparing the O.D. of the samples to the standard curve.

試劑盒供應(yīng)商：上海華壹生物科技有限公司