魚雌激素ELISA試劑盒實驗原理：本試劑盒用于測定魚血清，血漿,組織及相關液體樣本中激素（E）的含量。應用雙抗體夾心法測定標本中魚雌激素（E）水平。用純化的魚雌激素（E）抗體包被微孔板，制成固相抗體，往包被單抗的微孔中依次加入雌激素（E），再與HRP標記的雌激素（E）抗體結合，形成抗體-抗原-酶標抗體復合物，經過*洗滌后加底物TMB顯色。TMB在HRP酶的催化下轉化成藍色，并在酸的作用下轉化成zui終的黃色。顏色的深淺和樣品中的雌激素（E）呈正相關。用酶標儀在450nm波長下測定吸光度（OD值），通過標準曲線計算樣品中魚雌激素（E）濃度。
Fish Estrogen(E)ELISA Kit
Purpose
This kit allows for the determination of E concentrations in Fish serum, blood plasma, tissue and other biological fluids.
Principle of the assay
The kit assay Fish E level in the sample，use Purified Fish E to coat microtiter plate wells, make solid-phase antibody, then add E to wells, Combined E antibody which With HRP labeled , become antibody - antigen - enzyme-antibody complex, after washing Compley, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of E in the samples is then determined by comparing the O.D. of the samples to the standard curve.
試劑盒供應商：上海華壹生物科技有限公司