人皮質酮CORT酶聯免疫分析試劑盒實驗原理：本試劑盒用于測定人血清，細胞上清及相關液體樣本中皮質酮 (CORT)的含量。應用雙抗體夾心法測定標本中人皮質酮 (CORT)水平。用純化的人皮質酮 (CORT)抗體包被微孔板，制成固相抗體，往包被單抗的微孔中依次加入皮質酮 (CORT)，再與HRP標記的皮質酮 (CORT)抗體結合，形成抗體-抗原-酶標抗體復合物，經過*洗滌后加底物TMB顯色。TMB在HRP酶的催化下轉化成藍色，并在酸的作用下轉化成zui終的黃色。顏色的深淺和樣品中的人皮質酮 (CORT)呈正相關。用酶標儀在450nm波長下測定吸光度（OD值），通過標準曲線計算樣品中人皮質酮 (CORT)濃度。

Human Corticosterone (CORT) ELISA Kit

Principle of the assay

The kit assay Human CORT level in the sample，use Purified Human CORT to coat microtiter plate wells, make solid-phase antibody, then add CORT to wells, Combined antibody which With HRP labeled goat anti-Mouse become antibody - antigen - enzyme-antibody complex, after washing Compley, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of CORT in the samples is then determined by comparing the O.D. of the samples to the standard curve.

上海華壹生物科技有限公司
Shanghai HuaYi Bio-technology Co.,Ltd