亚洲中文久久精品无码WW16,亚洲国产精品久久久久爰色欲,人人妻人人澡人人爽欧美一区九九,亚洲码欧美码一区二区三区

上海乾思生物科技有限公司
中級會員 | 第16年

18916423352

細胞庫
優(yōu)勢品牌
Encapsula NanoSciences Quidel Bioporto Salimetrics Research Products International Corp Quanta BioDesign Peninsula Laboratories International Golden West Diagnostics Ocean NanoTech HaemoScan affinitybiologicals Cytodiagnostics Innovagen AB CLIAwaived Svar Abbexa ABMgood AffinityImmuno AGScientific AHF analysentechnik AG Alomone 英國Antibodies AstaTech Avidity Badrilla BioAcademia Biomatik Biosynth BioTeZ Biotrend Calbiotech CaroteNature Cell Sciences Click Chemistry Tools Cohesion Biosciences CordenPharma Cosmo Bio Creative Biomart Crystal Chem Crystalgen Diagnovum DiaMetra Dianova Diatheva Eagle Manufacturing Echelon Biosciences Edgebio Emfret Epoch Life Science Equitech-Bio Eramask Euro Diagnostica Exocell Fine Science Tools GenwayBio Giotto Biotech Glen Research Glycosynth Gold Biotechnology Golden West Biologicals Haemtech(HTI) Herolab IHC WORLD ImmuChrom GmbH Immunostep Immunotools Immunovision INCELL Corporation Innovative Research Innovrsrch(IRA) Insitus Iris Biotech GmbH Isosep IST Engineering Inc J.G. Finneran Kainos Kamiya Biomedical Labor Diagnostika Nord Larodan Laysanbio Lc laboratories Lee BioSolutions LGC Biosearch Technologies Lifecore List Labs lumiprobe Macrocyclics Medix Biochemica Meridian Life Science Microbix Milenia Biotec Moscerdam Mybiosource Nanoimmunotech Nanoprobes Neuro Probe Paratechs Physitemp Polypure Polysciences Probetex Progen ProScience Inc ProSpec QAbio Qorpak Quanta BioSciences QuickZyme RevMAb Biosciences SCHMIDT BioMedTech Seven Hills Foundation Smartox Biotechnology Spherotech Surgimax Swant SYSY Ted Pella Torpac Trilink USBio VIDIA Ltd ViroStat Virusys VP-scientific Xcessbio XENOMETRIX Zeptometrix ZYTOVISION
自產(chǎn)ELISA試劑盒,試劑盒
進口ELISA試劑盒
人細胞,人細胞系,人細胞株
3h biomedical,3H Biomedical AB,3hbiomedical,3-M Company,4adi,4rtilab,5 prime,5prime a & g pharmaceuticals,A&D Weighing,Aalto Scientific,Aalto Scientific ltd,Aalto Scientific,ltd.,aaltobioreagents,aaltoscientific,aat bioquest AAT Bioquest Inc,AB Biolabs,ab peptides,AB Sciex,Abaxis,Abazyme,Abberior,abbexa abbiotec,Abbkine,abbomax,Abbott Molecular,abcam,ABclonal,ABclonal Inc.,abcore-inc abcr GmbH,abcrGmbH,abcysonline,ABD,ABD Bioquest,Inc,abd serotec,ab-direct,abdserotec Abeomics,abfrontier,abgab,abgene,abgent,ABI Applied Biosystems,abiocode,abionline Abkem Iberia,ABL,Inc,Abliance,abm Inc.,abmart,abmgood,Abmole,Abmole Bioscience Inc. abnova,Abraxis,Absolute Antibody,ABT Beads,abt beads technologies,ABTbeads,abvision,Abways abzoom,academy biomed,academy bio-medical,acanthus research,acanthusresearch,ACC,accelagen,Accelagen inc. Access Biologicals,LLC,accessbiologicals,acciusa,accu-chek,AccuDiag Allergy,AccuDiag Anemia,AccuDiag Autoimmune Diseases,AccuDiag Bone Metabolism AccuDiag Thyroid,accumax accutase,Accumetrics,Accurate,accurate chemical,Accurate Wirecraft Company,accuratechemical,accustandard acdbio,Ace Glass,Ace Hose and Rubber Co,ACEA Biosciences,ACL Incorporated,ACME Microspheres Inc,ACMEmicrospheres,acris acris antibodies,Acris Antibodies GmbH,acris-antibodies,ACROBiosystems,acrometrix,acros,Acros Organics,ACT Test Panels LLC actc,actgene,active motif,activemotif,Adam Equipment,Inc,AddexBio,Addexbio Technologies,addgene ademtech,adg,ADI,ADInstruments,adipogen,Adolf Kuhner,adooq,Advance Tabco advanced immunochemical,advanced immunochemical inc,advanced targeting systems,Advansta,Advansta Inc.,Advansta,Inc,Advantech,Advent Electric Inc adventbio,advnt,advtechind,advy chemical,advy chemical Purified Proteins,advychemical Polyclonal Antisera,aesku,aetltd affiland,affinity,affinity biologicals,affinity bioreagent,Affinity BioReagents,Affinity BioReagents abr,Affinity Biosciences,affinitybiologicals AffinityBioReagents,AffinityImmuno,affix scientific,affixscientific,affymetrix,ag scientific,Agar Scientific,agarscientific agdia,Agela Technologies,agilent,Agilent Seahorse,agilent technologies,agilet,agilet solutions,agiletsolutions agrenvec,agrisera,AGRO-BIO,agscientific,ahdiagnostics,ahf,AHF analysentechnik,AHF analysentechnik AG Air Technology Solutions,Airgas Safety,Air-Tite,AJAX SCIENTIFIC LTD,AK Limited,Akro-Mils,ALA Scientfic,alamarblue Alba Bioscience,Alconox Inc,aldevron,alerchek,alere,Alexis,ALEXIS Biochemicals,Alfa Aesar Aline Biosciences,AlineBiosciences,All Foils Incorporated,Allcells,allele biotech,allelebiotech,allen-bradley,AllerMAbs
大鼠細胞,大鼠細胞系
細胞株,細胞系,傳代細胞
抗體庫,小包裝,流式抗體
SIGMA現(xiàn)貨,SIGMA代理

免疫印跡

時間:2010/12/1閱讀:5285
分享:

免疫印跡又稱蛋白質(zhì)印跡,它是根據(jù)抗原抗體的特異性結(jié)合檢測復(fù)雜樣品中的某種蛋白的方法。是在凝膠電泳和固相免疫測定技術(shù)基礎(chǔ)上發(fā)展起來的一種新的免疫生化技術(shù)。由于免疫印跡具有SDS-PAGE的高分辨力和固相免疫測定的高特異性和敏感性,現(xiàn)已成為蛋白分析的一種常規(guī)技術(shù)。免疫印跡常用于鑒定某種蛋白,并能對蛋白進行定性和半定量分析。
    言科生物根據(jù)多年的經(jīng)驗選用了目前市場上質(zhì)量與口碑較好的試劑,為廣大的客戶提供的WB服務(wù)。費用:每張膜1000元,客戶自己提供一抗,或者由我們公司代購,常規(guī)二抗免費提供,每張膜可以做6-8個樣品(各種陰性對照和陽性對照計入樣品數(shù)內(nèi)),承諾實驗周期為2周

主要實驗步驟如下:
1、蛋白質(zhì)準(zhǔn)備;
2、蛋白質(zhì)定量;
3、變性聚丙烯酰胺不連續(xù)凝膠電泳(SDS-PAGE):將準(zhǔn)備好的樣品液和預(yù)染蛋白marker分別上樣,標(biāo)準(zhǔn)加進*個孔中,電泳分離蛋白。 
4、蛋白質(zhì)轉(zhuǎn)移到PVDF膜,按Bio-Rad蛋白轉(zhuǎn)移裝置說明組裝濾紙凝膠纖維素夾層,30mA恒流條件下,4℃轉(zhuǎn)移過夜。
5、western blot膜的封閉和抗體孵育;
膜在5%脫脂奶粉溶液中室溫孵育1小時以封閉膜上的非特異結(jié)合。
封閉過的膜加入一抗室溫孵育1.5小時,抗原抗體結(jié)合。
加入HRP標(biāo)記的二抗體以結(jié)合一抗,室溫孵育膜1小時。加入HRP標(biāo)記的GAPDH抗體可同時檢測GAPDH含量。
6、western blot結(jié)果檢測:化學(xué)發(fā)光法檢測,膜與化學(xué)發(fā)光底物孵育,經(jīng)X膠片曝光顯影。圖片掃描保存為電腦文件,并用GIS1000分析軟件將圖片上每個特異條帶灰度值的數(shù)字化;
7、western blot數(shù)據(jù)分析:目的蛋白的灰度值除以內(nèi)參GAPDF的灰度值以校正誤差,所得結(jié)果代表某樣品的目的蛋白相對含量;
8、提供實驗報告,包括詳細的實驗方法及實驗結(jié)果的相關(guān)數(shù)據(jù)。

 

乾思 生物elisa試劑盒網(wǎng)發(fā)布

會員登錄

×

請輸入賬號

請輸入密碼

=

請輸驗證碼

收藏該商鋪

X
該信息已收藏!
標(biāo)簽:
保存成功

(空格分隔,最多3個,單個標(biāo)簽最多10個字符)

常用:

提示

X
您的留言已提交成功!我們將在第一時間回復(fù)您~
撥打電話
在線留言