雙功能交聯劑LC-SPDP Crosslinker 的結構式

產品名稱：LC-SPDP

英文全稱：Succinimidyl 6-(3-[2-pyridyldithio]-propionamido)hexanoate

產品簡介：LC-SPDP crosslinker is a cleavable, water insoluble, amino and thiol (sulfhydryl) reactive het-erobifunctional protein crosslinker. The "long chain" LC-SPDP crosslinking reagent has a 15.7 angstrom spacer arm, compared to the 6.8 angstrom spacer arm of SPDP.

外  觀：白色干燥粉末

分子量: 425.5

分子式：C18H23O5N3S2

溶解性：DMSO or DMF

Spacer Length: 15.7 Å

結構式：

應用簡介：

The amine-reactive portion of SPDP reagents is the N-hydroxysuccinimide (NHS) ester. Reactions are most commonly performed in phosphate, carbonate/bicarbonate, or borate buffers at pH 7-8. Other buffers can be used provided they do not contain primary amines (or thiols or disulfide reducing reagents – see discussion of the sulfhydryl reactivity). The rate of reaction and degradation by hydrolysis increases with increasing pH; for example, the half-life of the NHS ester is several hours at pH 7 and less than 10 minutes at pH 9. NHS-ester reagents like SPDP and LC-SPDP have limited aqueous solubility and must be dissolved in organic solvent before adding them to a reaction mixture. Sulfo-NHS-ester reagents like Sulfo-LC-SPDP are water-soluble and can be added directly to aqueous reaction mixtures.

產品列表：

ANB-NOS Crosslinker     CAS：60117-35-3

Biotin-SS-NHS           CAS：142439-92-7 

Sulfo-NHS-SS-Biotin     CAS：325143-98-4

Sulfo-NHS-Biotin        CAS: 119616-38-5

Biotin-NHS              CAS: 35013-72-0

HPPH                    CAS：65330-63-4

EMCS                    CAS: 55750-63-5

DSP/DTSP                CAS: 57757-57-0

DSS                     CAS: 68528-80-3

SFB                     CAS：60444-78-2

SATP                    CAS：84271-78-3

SATA                    CAS: 76931-93-6

SIAB                    CAS：72252-96-1

GMBS                    CAS：80307-12-6

AMAS                    CAS：55750-61-3

Biotin-LC-LC-NHS        CAS：89889-52-1

LC-Biotin hydrazide     CAS：109276-34-8

Sulfo NHS LC Biotin     CAS：127062-22-0

Biotin-amine            CAS：111790-37-5

MSC-ONSU                CAS：57903-15-8 

N-Biotinyl Glycine      CAS：160390-90-9

SANPAH                  CAS:64309-05-3

Sulfo-NHS Acetate       CAS: 152305-87-8

DTSSP crosslinker       CAS：142702-31-6

Biotin-Azide            CAS：908007-17-0

Biotin hydrazide        CAS：66640-86-6  

Biotin-PFP ester        CAS：120550-35-8

SMPB                    CAS: 79886-55-8

PMPI                    CAS：123457-83-0

LC-SMCC                 CAS：125559-00-4

TMEA                    CAS：139112-38-2

SMCC                    CAS: 64987-85-5

DTME                    CAS: 71865-37-7

GMBS                    CAS: 80307-12-6

EMCH                    CAS: 81186-33-6

Sulfo-SMCC              CAS: 92921-24-9

BMPS                    CAS: 55750-62-4

LC-SPDP

MBS                     CAS: 58626-38-3

Sulfo-NHS               CAS: 106627-54-7

BS3                     CAS: 82436-77-9

BS2G                    CAS: 881415-72-1

BSG                     CAS: 79642-50-5

DTSSP                   CAS: 81069-02-5

EGS                     CAS: 70539-42-3

Sulfo-EGS               CAS:142702-32-7

DSSeb                   CAS：23024-29-5

PDPH

SBA                     CAS: 42014-51-7

SIA                     CAS: 39028-27-8

SMPH

SPDP                    CAS：68181-17-9

Sulfo-LC-SPDP

Sulfo-MBS               CAS:92921-25-0

Sulfo-SANPAH            CAS: 102568-43-4

BS2G-d4

BS3-d4

DSG-d4                  CAS:910292-86-3

DSS-d4

DSP-d8                  CAS:1639836-65-9

Biotin Hydrazide        CAS: 66640-86-6

Sulfo-NHS-Biotin        CAS: 119616-92-5

Sulfo-NHS-LC-Biotin     CAS: 128062-22-0

Sulfo-NHS-SS-Biotin     CAS: 202057-28-1

HPG                     CAS: 24645-80-5

Pepsin                  CAS: 9001-75-6

Chymotrypsin            CAS: 9004-07-3

Trypsin                 CAS: 9002-07-7

Streptavidin            CAS: 9013-20-1

Horseradish Peroxidase (HRP)   CAS: 9003-99-0

Alkaline Phosphatase 35K Units CAS: 9001-78-9

Purified Polysorbate 20

Purified Polysorbate 80

Purified Nonidet P-40

Purified Triton X-100

Purified Triton X-114

TCEP-HCl Reducing Agent CAS: 51805-45-9

DTT                     CAS: 3483-12-3

pNPP                    CAS: 4264-83-9

Sodium meta-Periodate   CAS: 7790-28-5

Succinylacetone         CAS: 51568-18-4

Dimethyl sulfoxide      CAS: 67-68-5

Dimethylformamide       CAS: 68-12-2

MES Monohydrate         CAS: 145224-94-8

Sodium Borate Buffer    CAS: 1303-96-4

雙功能交聯劑LC-SPDP Crosslinker 的結構式

產品名稱：LC-SPDP

英文全稱：(succinimidyl 6-[3(2-pyridyldithio)propionamido]hexanoate)

General description

The amine-reactive portion of SPDP reagents is the N-hydroxysuccinimide (NHS) ester. Reactions are most commonly performed in phosphate, carbonate/bicarbonate, or borate buffers at pH 7-8. Other buffers can be used provided they do not contain primary amines (or thiols or disulfide reducing reagents – see discussion of the sulfhydryl reactivity). The rate of reaction and degradation by hydrolysis increases with increasing pH; for example, the half-life of the NHS ester is several hours at pH 7 and less than 10 minutes at pH 9. NHS-ester reagents like SPDP and LC-SPDP have limited aqueous solubility and must be dissolved in organic solvent before adding them to a reaction mixture. Sulfo-NHS-ester reagents like Sulfo-LC-SPDP are water-soluble and can be added directly to aqueous reaction mixtures.

我們試驗試劑胺活性部分N-羥基琥珀酰亞胺（NHS）酯。反應通常在磷酸鹽、碳酸鹽/碳酸氫鹽或硼酸鹽緩沖液中進行。如果不含伯胺（或硫醇或二硫鍵還原試劑）-見巰基反應性，可以使用其他緩沖劑。反應速率和水解降解率隨pH值的增加而增加；例如，ph值為7的ph值為9小時，pH值小于10分鐘。NHS酯試劑SPDP和lc-spdp有限的水溶解度和溶解在有機溶劑中之前，必須添加到反應混合物。磺NHS酯試劑磺LC SPDP是水溶性的，可以直接加入到反應混合物的水

簡介：LC-SPDP crosslinker is a cleavable, water insoluble, amino and thiol (sulfhydryl) reactive het-erobifunctional protein crosslinker. The "long chain" LC-SPDP crosslinking reagent has a 15.7 angstrom spacer arm, compared to the 6.8 angstrom spacer arm of SPDP.

結構式：

外觀：白色干燥粉末

Molecular Weight: 425.5

分子式C18H23O5N3S2

溶解性：DMSO or DMF

Spacer Length: 15.7 Å

Alternative Name: Succinimidyl-6-(3-[2-pyridyldithiol-propionamido) hexanoate