詳細介紹
巰基反應性熒光染料Cy5.5馬來酰亞胺
Cyanine 5.5應用非常廣泛,不僅可用于生物大分子的標記,還被用作一些小分子的衍生化試劑。maleimide(馬來酰亞胺)常被用來標記巰基,Cyanine5.5 Maleimide與其他標記巰基的熒光試劑(如FITC,TRITC等)相比,它反應選擇更高。Cyanine5.5 Maleimide是近紅外染料,除了用于標記多肽,蛋白和寡核苷酸氨基外,也常被用于小動物活體成像。因為它避開了很多物質都有吸收的可見光區,吸收的近紅外光在生物組織中的穿透深度較大,而激發的熒光受生物組織本底的影響較少,所以能夠在深層組織產生信號。尤其是在700~900 nm的范圍中,水和血紅蛋白的吸收都很少,近紅外光可以深入組織內部多達15 cm。同時,這類染料還擁有紫外光區染料和同位素標記無法具備的生物安全性。Cyanine 5.5 (cy 5.5)是常用的近紅外熒光染料。
cyanine 5.5 Maleimide需要共溶劑,但由于其疏水性比Cyanine7弱,應用更為廣泛。陜西新研博美生物提供三乙胺鹽酸鹽(triethylammonium salts)形式的cyanine 5.5 Maleimide,較其他公司鉀鹽形式的cyanine 5.5 Maleimide更易容,在光學性質,反應性相同的情況下,擁有更好的溶解性。
cyanine 5.5 Maleimide(馬來酰亞胺)廣譜學性質與Alexa Fluor 680 Maleimide和DyLight 680 Maleimide類似,可作為其替代物。
巰基反應性熒光染料Cy5.5馬來酰亞胺
技術資料
1.Ex(nm):678
2.Em(nm):701
3.分子量:1153.20
4.溶劑:DMSO
5.結構式:
熒光染料分類:
Dye NHS esters
BDP FL NHS ester
BDP R6G NHS ester
BDP TMR NHS ester
BDP TR NHS ester
Coumarin 343 X NHS ester
Cyanine3 NHS ester
Cyanine3.5 NHS ester
Cyanine5 NHS ester
Cyanine5.5 NHS ester
Cyanine7 NHS ester
Cyanine7.5 NHS ester
FAM NHS ester, 6-isomer
Pyrenebutyric acid NHS ester
ROX NHS ester, pure 6- isomer
Sulfo-Cyanine3 NHS ester
Sulfo-Cyanine5 NHS ester
Sulfo-Cyanine5 bis-NHS ester
Sulfo-Cyanine5.5 NHS ester
Sulfo-Cyanine7 NHS ester
Dye azides
BDP FL azide
BDP TMR azide
Coumarin 343 X azide
Coumarin 343 azide
Cyanine3 azide
Cyanine3.5 azide
Cyanine5 azide
Cyanine5.5 azide
Cyanine7 azide
Cyanine7.5 azide
FAM azide, 5-isomer
FAM azide, 6-isomer
PEP azide
Perylene azide
Pyrene azide 1
Pyrene azide 2
Pyrene azide 3
R110 azide, 5- isomer
R110 azide, 6- isomer
ROX azide, 5- isomer
Sulfo-Cyanine3 azide
Sulfo-Cyanine5 azide
Sulfo-Cyanine5.5 azide
Sulfo-Cyanine7 azide
TAMRA azide, 5-isomer
Dye alkynes
1-Ethynyl pyrene
3-Ethynyl perylene
BDP FL alkyne
BDP TMR alkyne
Cyanine3 alkyne
Cyanine5 alkyne
Cyanine5.5 alkyne
Cyanine7 alkyne
Cyanine7.5 alkyne
FAM alkyne, 5-isomer
FAM alkyne, 6-isomer
ROX alkyne, 5-isomer
Sulfo-Cyanine3 alkyne
Sulfo-Cyanine5 alkyne
Sulfo-Cyanine5.5 alkyne
Sulfo-Cyanine7 alkyne
TAMRA alkyne, 5-isomer
Dye maleimides
BDP FL maleimide
BDP TMR maleimide
Cyanine3 maleimide
Cyanine5 maleimide
Cyanine5.5 maleimide
Cyanine7 maleimide
Cyanine7.5 maleimide
FAM maleimide, 6-isomer
Pyrene maleimide
Sulfo-Cyanine3 maleimide
Sulfo-Cyanine5 maleimide
Sulfo-Cyanine7 maleimide
Dye hydrazides
BDP FL hydrazide
Cyanine3 hydrazide
Cyanine5 Boc-hydrazide
Cyanine5 hydrazide
Cyanine5.5 hydrazide
Cyanine7 hydrazide
Cyanine7.5 hydrazide
Pyrene hydrazide
Carboxylic acids
BDP FL carboxylic acid
BDP R6G carboxylic acid
BDP TMR carboxylic acid
Coumarin 343 X carboxylic acid
Cyanine3 carboxylic acid
Cyanine3.5 carboxylic acid
Cyanine5 carboxylic acid
Cyanine5.5 carboxylic acid
Cyanine7 carboxylic acid
Cyanine7.5 carboxylic acid
Pyrenebutyric acid
Sulfo-Cyanine3 carboxylic acid
Sulfo-Cyanine5 carboxylic acid
Sulfo-Cyanine5.5 carboxylic acid
Sulfo-Cyanine7 carboxylic acid
Sulfo-Cyanine7.5 carboxylic acid