詳細(xì)介紹
背景資料:
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This e encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This e is located in the class II region of the MHC (major histocompatibility complex). Expression of this e is induced by gamma interferon and this e product replaces catalytic subunit 1 (proteasome beta 6 subunit) in the immunoproteasome. Proteolytic processing is required to erate a mature subunit. [provided by RefSeq, Mar 2010] |
僅供科研實(shí)驗(yàn)商品屬性:
產(chǎn)品名稱 | 保存條件 | 規(guī)格 |
LMP2抗體 | Shipped at 4℃. Store at -20 °C for one year | 50ul 100ul 200ul |
商品介紹:
產(chǎn)品分類:抗體 / 一抗 英文名稱:LMP2 中文名稱 低分子質(zhì)量蛋白2抗體 英文別名 Proteasome 20S LMP2; Large multifunctional peptidase 2; LMP 2; LMP2; Low molecular mass protein 2; Macropain chain 7; MGC70470; Multicatalytic endopeptidase complex chain 7; Proteasome beta 9 subunit; Proteasome catalytic subunit 1i; Proteasome chain 7; Proteasome related e 2; Proteasome subunit beta 6i; Proteasome subunit beta type 9; PSMB 9; PSMB9; PSB9_HUMAN. 交叉反應(yīng):Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit 標(biāo)記: Unconjugated 抗體來源: Rabbit 抗體類型: Polyclonal 蛋白細(xì)胞定位: 細(xì)胞核,細(xì)胞漿 純化方法: affinity purified by Protein A 亞型 IgG 性狀: Liquid 濃度: 1mg/ml 儲存液: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. 保存條件: Shipped at 4℃. Store |
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實(shí)驗(yàn)原理
(1)特異性結(jié)合抗原:抗體本身不能直接溶解或殺傷帶有特異抗原的靶細(xì)胞,通常需要補(bǔ)體或吞噬細(xì)胞等共同發(fā)揮效應(yīng)以清除病原微生物或?qū)е虏±頁p傷。然而,抗體可通過與病毒或毒素的特異性結(jié)合,直接發(fā)揮中和病毒的作用。
(2)活補(bǔ)體:IgM、IgG1、IgG2和IgG3可通過經(jīng)典途徑激活補(bǔ)體,凝聚的IgA、IgG4和IgE可通過替代途徑激活補(bǔ)體。
(3)結(jié)合細(xì)胞:不同類別的免疫球蛋白,可結(jié)合不同種的細(xì)胞,參與免疫應(yīng)答。
(4)可通過胎盤及粘膜:免疫球蛋白G(IgG)能通過胎盤進(jìn)入胎兒血流中,使胎兒形成自然被動
免疫。免疫球蛋白A(IgA)可通過消化道及呼吸道粘膜,是粘膜局部抗感染免疫的主要因素。
(5)具有抗原性:抗體分子是一種蛋白質(zhì),也具有刺機(jī)體產(chǎn)生免疫應(yīng)答的性能。不同的免疫球蛋白分子,各具有不同的抗原性。
(6)抗體對理化因子的抵抗力與一般球蛋白相同:不耐熱,60~70℃即被破壞。各種酶及能使蛋白質(zhì)凝固變性的物質(zhì),均能破壞抗體的作用。抗體可被中性鹽類沉淀。在上??捎昧蛩徜@或硫酸鈉從免疫血清中沉淀出含有抗體的球蛋白,再經(jīng)透析法將其純化。
實(shí)驗(yàn)步驟:
一、準(zhǔn)備好試劑與儀器: | 二、實(shí)驗(yàn)步驟 |
磷酸鹽緩沖鹽水、熒光標(biāo)記的抗體溶液、緩沖甘油、搪瓷桶三只、有蓋搪瓷盒一只、熒光顯微鏡、玻片架、濾紙、37℃溫箱等。 | 1.滴加0.01mol/L,pH7.4的PBS于待檢標(biāo)本片上,十分鐘后棄去,使標(biāo)本保持一定濕度。 |
小鼠腎素(Renin)ELISA試劑盒 ,英文名: Renin ELISA Kit
小鼠生長因子(GH)ELISA檢測試劑盒Mousegrowthhormone,GHELISAKIT 96T/48T
人肝臟型脂肪酸結(jié)合蛋白(L-FABP)免疫試劑盒 Human L-FABP ELISA Kit
Rathepatocytegrowthfactor,HGFELISAKit大鼠肝細(xì)胞生長因子(HGF)ELISA試劑盒規(guī)格:96T/48T
BJ5183細(xì)菌1毫升
MouseIerleukin11,IL-11ELISA試劑盒小鼠白介素11(IL-11)ELISA試劑盒規(guī)格:96T/48T
Anti-PDE4D/FITC 熒光素標(biāo)記0酸二酯酶4D抗體IgGMulti-class antibodies規(guī)格: 0.2ml
Rabbit Anti-Dog IgG/FITC 熒光素標(biāo)記兔抗狗IgGMulti-class antibodies規(guī)格: 0.3ml
DNA甲基轉(zhuǎn)移酶-3α抗體 Anti-Dnmt3a 0.1ml
Goat Anti-human IgG/FITC FITC標(biāo)記的羊抗人IgG 0.3ml
FRG2B 英文名稱: FRG2B蛋白抗體 0.2ml
Rhesus antibody Rh phospho-TRADD(Ser269) 0酸化壞死因子受體1相關(guān)死亡域蛋白抗體 規(guī)格 0.1ml
Rabbit Anti-Dog IgG/FITC 熒光素標(biāo)記兔抗狗IgGMulti-class antibodies規(guī)格: 0.3ml
LMP2抗體馬皮疽組織胞漿菌PCR檢測試劑盒 規(guī)格 50T 英文; Histoplasm farcinimosumPCR
馬泰勒梨形蟲PCR檢測試劑盒原 規(guī)格 50T 英文; Theileria equiPCR(Babesia equi)
麥芽香肉桿菌PCR檢測試劑盒 規(guī)格 50T 英文; Carnobacterium maltaromaticumPCR
貓免疫缺陷病毒PCR檢測試劑盒 規(guī)格 50T 英文; Feline Immunodeficiency Virus(FIV)RTPCR
動物抗體功能分類:
①豬抗體:豬瘟抗體,豬藍(lán)耳抗體,豬圓環(huán)病毒抗體,豬偽狂犬抗體,豬細(xì)小病毒抗體,豬口蹄疫抗體,豬流感抗體等。
②禽抗體:小鵝瘟抗體,鴨肝抗體抗體,鴨漿膜炎抗體,抗體,新城疫抗體等
③??贵w:牛口蹄疫抗體,奶牛乳房炎抗體,牛流行熱抗體,牛病毒性腹瀉抗體,牛出血性敗血癥抗體等
④羊抗體:羊痘抗體,羊口蹄疫抗體,羊小反芻獸疫抗體,羊快疫抗體,羊腸毒血癥抗體,羊猝疽抗體,羊黑疫抗體等。
⑤犬抗體:犬狂犬病抗體、犬瘟熱抗體、犬副流感抗體、犬腺病毒抗體與犬細(xì)小病毒病抗體,狐貉水貂的偽狂犬抗體、細(xì)小病毒抗體、乙腦抗體等。