目錄:愛必信(上海)生物科技有限公司>>細胞生物學>>重組蛋白>> abs00837Recombinant Human SCF(2ug)
供貨周期 | 一周 | 規格 | 2ug |
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貨號 | abs00837 |
概述 | |
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種屬 | Human |
形態 | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
來源 | Escherichia coli. |
氨基酸序列 | EGICRNRVTN NVKDVTKLVA NLPKDYMITL KYVPGMDVLP SHCWISEMVV QLSDSLTDLL DKFSNISEGL SNYSIIDKLV NIVDDLVECV KENSSKDLKK SFKSPEPRLF TPEEFFRIFN RSIDAFKDFV VASETSDCVV SSTLSPEKDS RVSVTKPFML PPVA |
內毒素水平 | Less than 1 EU/μg of Recombinant HumanSCF as determined by LAL method. |
活性 | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 × 105 IU/mg. |
Accession # | P21583 |
預期分子量 | Approximay 18.5 kDa, a single non-glycosylated polypeptide chain containing 164 amino acids. |
性能 | |
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溶解方法 | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
保存方法 | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
純度 | > 97 % by SDS-PAGE and HPLC analyses. |
Gene IDs | 4254 |
參考文獻 | |
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1. Ronnstrand L. 2004. Cell Mol Life Sci. 61:2535-48.<br/>2. Anderson DM, Williams DE, Tushinski R, et al. 1991. Cell Growth Differ. 2:373-8.<br/>3. Brannan CI, Lyman SD, Williams DE, et al. 1991. Proc Natl Acad Sci U S A. 88:4671-4.<br/>4. Okayama Y, Kawakami T. 2006. Immunol Res. 34:97-115. |