目錄:愛必信（上海）生物科技有限公司>>細胞生物學>>重組蛋白>> abs00838Recombinant Human G-CSF（2ug）
| 供貨周期 | 一周 | 規格 | 2ug |
|---|---|---|---|
| 貨號 | abs00838 |
| 概述 | |
|---|---|
| 種屬 | Human |
| 形態 | Lyophilized from a 0.2 μm filtered concentrated solution in 10mM sodium acetate buffer, containing 5 % trehalose, pH 4.0. |
| 來源 | Escherichia coli. |
| 氨基酸序列 | TPLGPASSLP QSFLLKCLEQ VRKIQGDGAA LQEKLCATYK LCHPEELVLL GHSLGIPWAP LSSCPSQALQ LAGCLSQLHS GLFLYQGLLQ ALEGISPELG PTLDTLQLDV ADFATTIW MEELGMAPAL QPTQGAMPAF ASAFQRRAGG VLVASHLQSF LEVSYRVLRH LAQP |
| 內毒素水平 | Less than 1 EU/μg of Recombinant HumanG-CSF as determined by LAL method. |
| 活性 | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine NFS-60 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 × 107 IU/mg. |
| Accession # | P09919 |
| 預期分子量 | Approximay 18.7 kDa, a single non-glycosylated polypeptide chain containing 174 amino acids. |
| 性能 | |
|---|---|
| 溶解方法 | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| 保存方法 | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 純度 | > 98 % by SDS-PAGE and HPLC analyses. |
| Gene IDs | 1440 |
| 參考文獻 | |
|---|---|
| 1. Scarffe JHandKamthan A. 1990. Cancer Surv, 9: 115-30.<br/>2. Fattorini L, Xiao Y, Li B, et al. 1994. J Med Microbiol, 40: 129-33.<br/>3. Tsavaris N, Kosmas C, Gouveris P, et al. 2004. Med Sci Monit, 10: PI24-8.<br/>4. Shochat E, Rom-Kedar V, Segel LA. 2007. Bull Math Biol, 69: 2299-338.<br/>5. Cornish AL, Campbell IK, McKenzie BS, et al. 2009. Nat Rev Rheumatol, 5: 554-9. | |
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