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Epicypher新品 | 核酸提取緩沖液
CUTANA™ 細胞核提取緩沖液是CUT&RUN和CUT&Tag實驗中，用于從培養的細胞和組織中提取細胞核的必需試劑。這種緩沖液可與多種真核細胞和組織兼容。以下是使用該緩沖液成功進行細胞核分離的細胞和組織類型：
mouse NIH3T3 fibroblast cells | human A549 non-small cell lung cancer (NSCLC) cells |
human K562 leukemia cells | human NCI-H1299 non-small cell lung cancer (NSCLS cells) |
human bone marrow derived macrophages | human TIG-1 fetal lung cells |
human monocyte derived macrophages | human LoVo colorectal cancer cells |
human MV-4-11 macrophage cells | human LNCaP prostate carcinoma cells |
human SUM149 triple negative breast cancer (TNBC) cells | human renal primary cells |
human GM24385 B-lymphocyte (aka HG002) cells | human peripheral blood mononuclear cells (PBMCs) |
human MCF7 breast cancer cells | human intestinal tissue |
human MDA-MB-231 breast cancer cells | human HEPM embryonic cells |
human SK-MEL-2 melanoma cells |
只需將試劑盒中的Pre-Nuclei Extraction Buffer和亞精胺與蛋白酶抑制劑混合即可完成該試劑的制備。CUTANA™ 細胞核提取緩沖液針對CUT&RUN和CUT&Tag實驗流程進行了優化，從而獲得高質量的細胞核，助力您的基因組圖譜分析。
試劑盒組成
組分 | 貨號 |
CUTANA™ Pre-Nuclei Extraction Buffer | 21-1026a |
1 M Spermidine | 21-1026b |
保存條件
將Pre-Nuclei Extraction Buffer 4oC儲存, 1 M亞精胺-20oC儲存。
自收到之日起可穩定儲存6個月。
驗證數據
Figure 1: Nuclei Extraction
Nuclei were extracted from 3 different cell types: K562, NIH3T3, and LNCaP using the CUTANA™ Nuclei Extraction Protocol for CUT&RUN and CUT&Tag. Top 3 panels show cells before extraction. Starting cells are viable (bright white and round). Bottom 3 panels show nuclei extracted using the CUTANA™ Nuclei Extraction Buffer as indicated by positive Trypan Blue staining.
訂購詳情
貨號 | 產品名稱 | 規格 |
21-1026 | CUTANA™ Nuclei Extraction Buffer | 100 Reactions |
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