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High-Resolution, Strong and Weak Anion-Exchange Columns for the Separation of Proteins
The ProPac® SAX-10 is a nonporous, strong anion-exchange column (quaternary amine functional group). The ProPac WAX-10 column is a weak anion exchanger (tertiary amine functional group). Both are based on a nonporous core particle that provides pressure stability. A hydrophilic layer prevents unwanted secondary interactions and a grafted anion exchange surface provides pH control, selectivity, and fast mass transfer for high efficiency separation, as well as moderate capacity.
Unequalled resolution
High-efficiency peaks with excellent recovery
Higher capacity than other columns based on nonporous particles
Useful for characterization and quality control assessment of closely-related protein variants
Supports separation of proteins that differ by as little as one amino acid residue
Neutral hydrophilic coat that eliminates protein-resin hydrophobic interactions
Superior lot-to-lot and column-to-column reproducibility
ProPac SAX-10 and WAX-10 columns can resolve protein isoforms that differ by as little as one charged residue. Differences in selectivity between the WAX-10 and SAX-10 provide flexibility in maximizing resolution of closely related species.
All ProPac columns are manufactured and tested under the strictest specifications, resulting in unmatched column-to-column and lot-to-lot reproducibility. Dionex also offers these columns as lot select packages, containing three columns from one lot or three columns from three different lots, to assist quality control scientists in method validation.
| ProPac SAX-10 Strong Anion-Exchange Column for the High-Resolution Separation of Protein Isoforms Specifications | |
|---|---|
| Maximum operating pressure | 3000 psi (21 MPa) |
| Maximum operating conditions | 60 °C Recommended operating temperature:<40 °C |
| Typical operating conditions | UV detection at 1 mL/min Recommended flow rate: 1 mL/min |
| phase compatibility | pH 2–12; 80% compatible with acetonitrile, acetone, methanol. Compatible with nonionic and zwitterionic detergents. Incompatible with anionic detergents. |
| Resin composition | 10-µm-diameter core bead (55% cross-linked) grafted with a pellicular layer to which are attached the quaternary ammonium functional groups. |
| Anion-exchange capacity | < 100 µg protein on-column, depending upon the protein (4 × 250 mm analytical column) |
| Column construction | PEEK with 10-32 threaded ferrule-style end fittings. All components are nonmetallic. |
| ProPac WAX-10 Weak Anion-Exchange Column for the High-Resolution Analysis of Protein Variants Specifications | |
|---|---|
| Maximum operating pressure | 3000 psi (21 MPa) |
| Maximum operating temperature | 60 °C Recommended operating temperature:<40 °C |
| Typical operating conditions | UV detection at 1 mL/min Recommended flow rate: 1 mL/min |
| phase compatibility | pH 2–12; 80% compatible with acetonitrile, acetone, methanol. Compatible with nonionic and switterionic detergents. Incompatible with anionic detergents. |
| Resin composition | 10-µm-diameter core bead (55% cross-linked) grafted with a pellicular layer to which are attached the tertiary amine functional groups. |
| Anion-exchange capacity | < 100 µg protein on-column, depending upon the protein (4 × 250 mm analytical column) |
| Column construction | PEEK with 10-32 threaded ferrule-style end fittings. All components are nonmetallic. |