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For Hydrophilic Anionic Protein Separations
The ProPac® PA1 column supports the analysis and purification of hydrophilic anionic proteins and peptides. Proteins and peptides that are highly cationic (e.g., those with very high isoelectric points) may be resolved on this column, but some may exhibit substantial binding to the column.
Good for hydrophilic anionic proteins and peptides
Ideal for high-resolution separations of proteins with pI values from 3 to 11
Available in semipreparative format
Pellicular packing ensures high-efficiency and fast mass transport
ProPac PA1 Column for Hydrophilic Anionic Protein Separations Specifications Column construction PEEK with 10-32 threaded ferrule-style end fittings.
All components are nonmetallic.Dimensions ProPac PA1 Analytical Column:
4 × 250 mm
ProPac PA1 High-Speed Column:
4 × 50 mm
ProPac PA1 Semipreparative Column:
9 × 250 mmMaximum operating pressure 5000 psi Recommended operating temperature Ambient Typical operating conditions 1.0 mL/min gradient
Recommended flow rate: 0.9–1.5 mL/minphase compatibility Compatible with up to 2% common organic solvents.
Recommended eluents: buffers such as Tris, MOPS phosphate with a salt, such as chloride.Resin composition 10-µm-diameter pellicular substrate (2% cross-linked) agglomerated with a 200-nm MicroBead alkyl quaternary ammonium functionalized latex (5.6% cross-linked). Anion-exchange capacity N/A