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Analytical Column for Immobilized Metal Affinity Chromatography
The ProPac® IMAC-10 is an analytical HPLC column packed with 10 µm, nonporous, polymeric beads that are coated with a hydrophilic layer, then grafted with poly(IDA) chains. The poly(IDA) grafts are converted to metal-containing nanoparticles when the column is charged with metal. It is these nanoparticles that act as IMAC interaction sites for individual proteins and provide the ProPac IMAC-10 with its high resolving power.
State-of-the-art technology for reusable columns with metal tailored specificity
Resolve target proteins using a single column in a high resolution gradient run
Retention control by imidazole concentration or pH gradient
High purity separations of metal-binding proteins
Wide range of metal-specific applications
The ProPac IMAC-10 design maximizes the number of sterically accessible ligands for binding to proteins, ensuring that the proteins remain tightly bound during separation. It can be operated between pH 2–12 and is compatible with commonly used protein purification reagents (non-ionic detergents, reducing agents). It can be used under native or denaturing HPLC conditions
The ProPac IMAC-10 is shipped free from any metal and ready to be charged with the metal of your choice. To avoid contact of the metal solution with your system, charge the IMAC-10 with a loading column (an empty column body you fill with your metal solution, then place between the pump and the IMAC-10 column).
| ProPac IMAC Columns Specifications | |
|---|---|
| Maximum Operating Pressure | <3000 psi (21 MPa) |
| Temperature Range | <60oC Recommended Temperature: Controlled ambient |
| Typical Operating Conditions | <600 psi at 1.0 mL/min (4 × 250mm) Recommended flow rate: 1.0 mL/min |
| Phase Compatibility | pH 2–12, Compatible with most reagents used in IMAC protein purifications, including EtOH, Urea, NaCl, non-ionic detergents, glycerol, acetic acid, guanidine, HCl |
| Resin Compostion | 10 µm diameter nonporous polystyrene divinylbenzene substrate (55% crosslinking) with poly(IDA) grafts. |
| Metal Ion Capacity (µmol Cu) | >80 mmol Cu (4 × 250 mm analytical column) |
| Chelating Metals | Ni, Fe, Cu, others |
| Analytical Column Dimensions | 1 × 50 mm, 2 × 50 mm, 4 × 50 mm, 9 × 50 mm 2 × 250 mm, 4 × 250 mm, 9 × 250 mm, 22 × 250 mm |
| Loading Column Dimensions | 4 × 50 mm, 9 × 50 mm, 9 × 250 mm |