目錄:北京索萊寶科技有限公司>>細胞培養相關>>細胞培養試劑>> 12100-500細胞培養基 DMEM（H）（含雙抗）
| 供貨周期 | 現貨 | 規格 | 500ml |
|---|---|---|---|
| 貨號 | 12100 | 應用領域 | 醫療衛生,化工,生物產業,石油 |
| 主要用途 | 細胞培養基 |
細胞培養基 DMEM（H）（含雙抗）
DMEM培養基的配制方法：
1 制備新鮮三蒸水或Millipore超純水。
2 稱取所需量的干粉培養基，加入約終體積一半的三蒸水中;若配制一個包裝的培養液，在將整個包裝的干粉倒入三蒸水后，需用水洗包裝袋內面2次，倒入培養液中，以保證所有干粉都溶解成培養液。磁力攪拌或人工攪拌使之*溶解。
3 根據包裝袋上的要求補加所需量的碳酸氫鈉;根據實驗需要，添HEPES(5-20mmol/L)、谷氨酰胺和其他特殊物質。
4 加水定容到終體積。
5 必要時用1 mol/L 鹽酸和1 mol/L 氫氧化鈉調節pH。
6 用無菌0.22um濾膜過濾除菌，分裝于無菌血清瓶中，4℃冰箱保存。
配制好的培養液用前加入100U/mL青霉素和100U/mL鏈霉素，并根據需要加入血清(5%-20%)。
DMEM培養基的特點：
（1）氨基酸含量為依格爾培養基的2倍，且含有非必需氨基酸，如甘氨酸等；
（2）維生素含量為依格爾培養基的4倍；
（3）含有糖酵解途徑中的重要物質——丙酮酸；
（4）含有微量的鐵離子。
DMEM培養基應用廣泛。基于DMEM培養基中氨基酸、微生物含量豐富，并具有糖酵解中的丙酮酸和微量Fe離子，因此在細胞培養、疫苗生產等方面的應用為常見。
適用于細胞培養， 產品含4.5g/L 葡萄糖 ，含谷氨酰胺，不含丙酮酸鈉。質量穩定可靠。
DMEM （Dulbecco's Modified Eagle Medium） （high Glucose）
with 4.5g/L Glucose，with L- glutamine，without sodium pyruvate
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細胞培養基 DMEM（H）（含雙抗）
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