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描述:N-2添加劑是化學成分確定的,不含血清的,基于Bottenstein’s 的N1配方的添加劑。N-2添加劑主要用于Neurobasal®培養基。
它主要被推薦用于成神經細胞瘤以及周圍神經系統(PNS)與中樞神經系統(CNS)的原代細胞培養中有絲分裂后期神經元的生長和表達。N-2添加劑可以替代Bottenstein’s的N1添加劑。我們提供的N-2添加劑是100倍的濃縮體。它可用于添加了生長因子bFGF及EGF的Neurobasal®培養基,或DMEM培養基。
說明:僅供試驗使用,不可用于人或動物之醫療診斷。
常用規格:
Cell Type: | Neuronal Tumor Cells, Neuronal Cells |
Product Size: | 5 ml |
Classification: | Serum-Free |
Purity or Quality Grade: | Cell Culture Grade |
Shipping Condition: | Dry Ice |
Regulatory Statement: | For Research Use Only. Not for use in diagnostic procedures. |
儲存:存放在冰柜 (-5 to -30°C)。
技術資源 -培養基配方
N-2 Supplement (100X) liquid
For the growth and expression of post-mitotic neurons and tumor cells of neuronal phenotype.
N-2 supplement is a chemically defined, 100X concentrate of Bottenstein's N-2 formulation . This supplement is recommended for the growth and expression of neuroblastomas and for the survival and expression of post-mitotic neurons in primary cultures from both the peripheral nervous system (PNS) and the central nervous system (CNS). It can be used as a substitute for the N-1 Bottenstein formulation. N-2 medium appears to be selective for neuronal cell lines and does not support the growth of nonneuronal cell lines. (in Current Methods in Cellular Neurobiology: Volume 4, edited by Jeffery Barker, John Wiley & Sons, Inc.,1983.)
Catalog Number(s): 17502048
Components | Molecular Weight | Concentration (mg/L) | mM |
Proteins | |||
Human Transferrin (Holo) | 10000 | 10000 | 1 |
Insulin Recombinant Full Chain | 5807.7 | 500 | 0.0861 |
Other Components | |||
Progesterone | 314.47 | 0.63 | 0.002 |
Putrescine | 161 | 1611 | 10.01 |
Selenite | 173 | 0.52 | 0.00301 |
訂購信息:
貨號 | 名稱 | 產地 | 規格 | 報價 | *(元) | 庫存 |
17502-048 | N-2 Supplement (100X), Liquid | Invitrogen | 5ml | 1173 | 630 | 是 |