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Roche 11585614910DIG High Prime DNA Labeling and Detection Starter Kit II
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DIG High Prime DNA Labeling and Detection Starter Kit II
DIG High Prime DNA Labeling and Detection Starter Kit II|DNA高效地高辛標(biāo)記及檢測試劑盒II|地高辛標(biāo)記試劑盒|高效地高辛標(biāo)記試劑盒|羅氏地高辛標(biāo)記試劑盒
DNA高效地高辛標(biāo)記及檢測試劑盒II (Roche 貨號: 11585614910)
DIG-High Prime DNA Labeling and Detection Starter Kit II
1 kit for 12 labeling reactions and detection of 24 blots 10 ng to 3 μg per assay, blots of 100 cm2
Application
DIG-High Prime labeled probes can be used in northern, Southern-, and dot-blot analysis, as well as colony and plaque hybridizations. This kit offers random-primed labeling of DNA templates with DIG-11-dUTP, alkali-labile, and chemiluminescent detection of the DIG-labeled hybrids. This kit was assembled with convenience in mind, offering ready-to-use CSPD supplied with a dripping device for easy application, ready-made blocking solution, and DIG Easy Hyb granules. The DIG-High Prime mixture includes stabilized Klenow enzyme, nucleotides, primers, and reaction buffer, all in one convenient reagent.
Benefits
The High Prime method of labeling offers many benefits: in addition to the optimized labeling efficiency, plasmid, cosmid, and phage DNA, linearized or supercoiled can be used as templates, yielding the same labeling efficiency.
Product Description
Sensitivity and specificity: Standard assay uses 1 µg of linearized pBR328. It is usual to expect 0.8 µg labeled DNA after 1 h of labeling, and 2.3 µg labeled DNA after 20 h reaction. In a dot blot hybridization, 0.03 pg of homologous DNA is detectable with chemiluminescence (probe concentrations at 20 ng/ml). A single copy human gene (t-PA) is detectable in a Southern blot, loading 1 µg of digested placenta DNA.
Contents
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聯(lián)系人:王錦華QQ344843571
電話:86-021-51875103
傳真:86-021-4006768800-5599
手機(jī):15026509758
留言:發(fā)送留言
個性化:www.ybiotech.com.cn
手機(jī)站:m.ybiotech.com.cn
網(wǎng)址:www.yubiotech.com
商鋪:http://www.weixunsd.com/st251076/