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下面所有質(zhì)粒和菌株,均為本公司實(shí)驗(yàn)室親自驗(yàn)證過,可以提供,載體來(lái)源于promega,addgene,clontech,可提供質(zhì)粒圖譜,可提供近萬(wàn)種質(zhì)粒載體、菌株、基因和細(xì)胞株,并可提供實(shí)驗(yàn)技術(shù)外包服務(wù),如基因克隆、質(zhì)粒構(gòu)建、蛋白原核、真核表達(dá)及純,pQE 載體,原核表達(dá)載體,克隆載體 ,畢赤酵母表達(dá)載體,畢赤酵母宿主菌,釀酒酵母表達(dá)載體 ,釀酒酵母宿主菌,真菌表達(dá)載體,真核哺乳動(dòng)物熒光載體,真核哺乳動(dòng)物載體,植物表達(dá)載體,大腸桿菌宿主菌,枯草宿主菌
釀酒酵母表達(dá)載體
pYES2,pYES2/NT,pYES2/CT,pYES3,pYES6, pYCplac22-GFP,酵母載體pAUR123,pRS303TEF,pRS304, pRS305,pRS306,pY13TEF,pY14TEF,pY15TEF,pY16TEF,酵母基因重組表達(dá)載體pUG6, pSH47,酵母單雜載體pHISi,pLacZi,pHIS2, pGAD424, 酵母雙雜交系統(tǒng):釀酒酵母Y187,釀酒酵母AH109;質(zhì)粒pGADT7,pGBKT7;對(duì)照質(zhì)粒pGBKT7-53,pGBKT7-lam,pGADT7-T,PCL1,釀酒酵母菌株INVSc1,YM4271, AH109,Y187,Y190, 畢赤酵母表達(dá)載體pPIC9K,pPIC9K-His,pPIC3.5K,pPICZalphaA,B,C,pPICZA,B,C,pGAPZαA,pAO815,pPIC9k-His,pHIL-S1,pPink hc,配套畢赤酵母Pichiapink,畢赤酵母宿主X33,KM71,KM71H,GS115,
原核表達(dá)載體pQE30,31,32,40,60,61,62,等原核表達(dá)載體,包括pET系列,pET-GST,pGEX系列(含GST標(biāo)簽),pMAL系列pMAL-c2x,-c4x,-c4e,-c5x,-p5x,pBAD,pBADHis,pBADmycHis系列,pQE系列,pTrc99a,pTrcHis系列,pBV220,221,222,pTXB系列,pLLP-ompA,pIN-III-ompA(分泌型表達(dá)系列),pQBI63(原核表達(dá)帶熒光)pET3a, pET 3d, pET 11a, pET 12a, pET 14b, pET 15b, pET 16b, pET 17b, pET 19b, pET 20b, pET 21a,b,d, pET 22b, pET 23a, pET 23b, pET 24a,b, pET 25b, pET 26b, pET 27b, pET 28a,b, pET 29a, pET 30a, pET 31b, pET 32a, pET 35b, pET 38b, pET 39b, pET 40b, pET 41a,b pET 42a, pET 43.1a,b pET 44a, pET 49b pET302,303 pET His,pET Dsb,pET GST,pET Trx pQE2, pQE9 pQE30,31,32, pQE 40 pQE70 pQE80L pQETirs system pRSET-A pRSET-B pRSET-C pGEX4T-1,-2,-3,5x-1,6p-1,6p-2,2tk,3c pBV220,221,222 pTrcHisA,B,C pBAD24,34,43 pBAD HisA,B,C pPinPoint-Xa1,Xa2,Xa3 pMALc2x, p2x pBV220 pGEM Ex1, pGEM7ZF(+), pTrc99A, pTwin1, pEZZ18 pkk232-8,pkk 233-3,pACYC184,pBR322,pUC119 pTYB1,pTYB2,pTYB4,pTYB11 pBlueScript SK(+),pBlueScript SK(-) pLLP ompA, pINIIIompA, pMBP-P ,pMBP-C, 大腸桿菌冷激質(zhì)粒: pColdI pColdII pColdIII pColdTF 原核共表達(dá)質(zhì)粒:pACYCduet-1,pETduet-1,pCDFduet-1,pRSFduet-1 Takara公司大腸桿菌分子伴侶: pG-KJE8 pGro7 pKJE7 pGTf2 pTf16 大腸桿菌宿主細(xì)胞: DH5a JM101 JM103 JM105 JM107 JM109 JM110 *0 *0F BL21(DE3) HB101 ER2529 E2566 C2566 MG1655 XL-10gold XL blue M15 JF1125 K802 SG1117 BL21(AI) BL21(DE3)plysS TG1 TB1 DH5a(pir) Tuner(DE3) Bl21 codonplusRIPL Novablue(DE3) Rosetta Rosetta(DE3) Rosetta(DE3)plys Rosetta-gami(DE3) Rosetta-gamiB(DE3), Rosetta-gamiB(DE3)plysS Orgami(DE3) OrgamiB(DE3) HMS174(DE3)
植物表達(dá)/RNAi載體農(nóng)桿菌pBI121,pBI121-GFP,pBI101,pBI221,pSN1301, pUN1301,pRTL2 , pRTL2-GFP , pRTL2-CFP, pRTL2-RFP , pRTL2-YFP,pCAMBIA 1300, 1301, 1302,1303,1304,1305, 1381Z,1391Z,2300, 2301,3300,3301,pCAMBIA super1300,pCAMBIA super1300-GFP,pPZP212,pPZP2121,pPZP212-GFP,pGDG,RNAi載體pART27,pHANNIBAL,pKANNIBAL, pFGC5941,pTCK303, pTRV1,pTRV2, T-DNA插入載體(隨機(jī)突變體庫(kù))pSKI015,pSKI074,真菌ATMT載體pBIG2RHPH2-GUS-GFP,pBHt1
枯草芽孢桿菌表達(dá)載體pWB980,pHT43,pHP13,pHP43, pBE2,pMUTIN4,pUB110,pE194,pMA5, pMK3,pMK4,pHT304,pHY300PLK, pBest502,pDG1363,pSG1154,pAX01, pSAS144,pDL,pDG148-stu,pDG641, pAL12,pUCX05-bgaB,pHT01, 配套菌株BS 168,WB600,WB800,WB700, WB800N,1012,FZB42,1A747,廣宿主質(zhì)粒pVLT33
RNAi基因沉默干擾敲除載體pSilencer1.0,pSilencer 2.1-U6 hygro, pSilencer 3.1-H1 hygro,pSilencer 3.1-H1 neo, pSilencer 4.1-CMV neo, pSilencer 4.1-CMV puro pMIR-REPORT Luciferase RNAi載體(oligoengine) pSuper-puro RNAi逆轉(zhuǎn)錄病毒載體(clontech): RNAi-Ready pSIREN-Retro Q, RNAi-Ready pSIREN-RetroQ-ZsGreen(Luciferase shRNA Annealed Oligonucleotide) RNAi慢病毒載體(addgene): pLKO.1
基因cDNA文庫(kù)ORF表達(dá)克隆質(zhì)粒,
擁有目前中國(guó)zui大的cDNA克隆庫(kù)、覆蓋10萬(wàn)人、鼠全長(zhǎng)基因的cDNA克隆和超過25,000個(gè)ORF克隆表達(dá)載體。
哺乳動(dòng)物表達(dá)載體pcDNA3.1+/-,pcDNA4/HisMax B,pSecTag2 A,pVAX1,pBudCE4.1,pTracer CMV2,pcDNA3.1(-)/myc-His A ,pcDNA6-Myc/His B,pCEP4, pIRES,pIRESneo,pIRES hyg3,pCMV-myc,pCMV-HA,pIRES-puro3,pIRES-neo3,pCAGGS哺乳動(dòng)物雙雜交系統(tǒng)pACT,pBIND,pACT-MyoD,pBIND-Id,pG5luc,pCMV-BD, pCMV-AD, pBD-p53, pFR-luc,Cytotrap Two-Hybrid System:pSos, pSos MAFB, pMyr蛻皮激素誘導(dǎo)系統(tǒng)pIND, pVgRxR,LacSwith II哺乳動(dòng)物誘導(dǎo)表達(dá)系統(tǒng):pOPRSVI ,pOPI3CAT,pCMVLacI,GeneSwitch System:pSwitch哺乳動(dòng)物表面展示系統(tǒng):pDisplay, 四環(huán)素調(diào)控系統(tǒng)(Invitrogen):pcDNA4/TO/Myc-His A,pcDNA4/TO/Myc-His B,pcDNA4/TO/Myc-His C,pcDNA4/TO/Myc-His/LacZ,pcDNA6/TR四環(huán)素調(diào)控系統(tǒng)(Clontech):pTet-On,pTet-Off,pTRE2,pRevTRE,pRevTet-On,pRevTet-off信號(hào)通路報(bào)告載體:pGAS-TA-Luc,pSTAT3-TA-Luc, pISRE-TA-Luc, pTA-Luc,pIκB-EGFP,pNFAT-TA-Luc,pCaspase3-sensor,pAP1(PMA)-Luc;pGL4.26[luc2P/minP/Hygro],pGL4.29[luc2P/CRE/Hygro],pGL4.30[luc2P/NFAT-RE/Hygro],pGL4.75;p53-Luc,pAP-1-Luc, pNF-κB-Luc,pSRE-Luc,pFA2-Elk1,pFC-MEKK,pFR-luc,Gateway系統(tǒng)(invitrogen)pcDNA6.2-GWEmGFP-miR negative, pLenti 6/TR,pcDNA 6.2-GW EmGFP-miR,
乳酸菌表達(dá)載體及各種乳酸菌乳酸桿菌菌株,pNZ8148,pLEISS,pMG36e,pBBR1MCS-5,pBBR1MCS-6,pRV610,pLEM415,pHY300PLK,分泌型乳酸菌表達(dá)載體pVE5523,pPG611.1,pPG612.1等和乳酸桿菌菌株宿主菌NZ9000,MG1363,Lactobacillus casei 1.539,Lactobacillus casei,acidophilus NCFM,1.2,Lactobacillus sakei 23K,L.plantarum,L.rhamnosus GG,B.coagulans,Bifidobacterium bifidum,Bifidobacterium infantis,Lactococcus lactis M17,1663,Lactobacillus reuterii
廣宿主表達(dá)載體鏈球菌表達(dá)敲除載體假單胞菌表達(dá)載體pVLT33,pBBR1MCS-2,3,4,5,6, pJRD215,pJN105,pME6032,Cos載體pLAFR3,pMP2444(GFP), pHY300PLK,pRT102,pRL1063a, 轉(zhuǎn)座子載體pUT-miniTn5,pMGS100, pWHM10,pKC1139,pSET152, pOJ260,pPG611.1,pPG612.1,
腺病毒載體/慢病毒,逆轉(zhuǎn)錄病毒表達(dá)載體及包裝包膜質(zhì)粒,腺病毒系統(tǒng)(Stratagene): pAdEasy-1,pShuttle-CMV,pShuttle,pAdTrack, pAdTrack-CMV, pShuttle-IRES-hrGFP-1、pShuttle-IRES-hrGFP-2、pShuttle-CMV-lacZ, pShuttle-CMV-EGFP-C,pXC1, pBHGE3, 配套大腸桿菌BJ5183,293,293T cell line 腺相關(guān)病毒系統(tǒng)(Stratagene): pAAV-MCS,pAAV-RC,pHelper,pAAV-LacZ,pAAV-IRES-hrGFP,pCMV-MCS, 慢病毒載體:pLVX-DsRed-Monomer-N1,pLVX-IRES-ZsGreen1,pLVX-AcGFP1-N1,Lenti6/v5-EDST-EGFP,pWPXL, FUGW,pLentilox 3.7,RNAi-Ready pSIREN-Retro Q, RNAi-Ready pSIREN-Retro Q-ZsGreen, pSUPER.Retro-GFP/Neo,pSUPER-Retro-Neo, pSUPER.Retro-puro,PLNCX PLNCX2 pMSCV-HYG pMSCV-neo pMSCV-puro pLEGFP-C1 pLOX-CW-CRE pLOX-GFP-IRES-TK pRetroX-IRES-DsRedExpress, pLVX-IRES-mCherry質(zhì)粒載體。 病毒包裝:二質(zhì)粒包裝系統(tǒng)一: psPAX2 pMD2.G 二質(zhì)粒系統(tǒng)二: pCMV-dR8.2 dvpr pCMV-VSVG 三質(zhì)粒包裝系統(tǒng)具有更高的包裝效率: pMDLg/pRRE pRSV-Rev pMD2.G Invitrogen包裝系統(tǒng): PLP1 PLP2 PLP/VSVG
基因重組載體敲除載體自殺質(zhì)粒RED重組系統(tǒng)載體pKD3,pKD13,pKD4,pCP20,pKD46, pCVD442,pk18mobsacB,p2NIL, pMAD(革蘭氏陽(yáng)性菌基因敲除),pBT2,RN4220,pKC1139,pOJ260, pJQ200SK,pSET152,pWHM10, pk18mobsacB, pGOAL17,pGOAL19,基因打靶載體PL253,PL451,PL452,PL453,配套菌株EL250,EL350,pMLM290,292, KJBAC1,pST1374
熒光蛋白表達(dá)載體熒光定位質(zhì)粒報(bào)告基因載體pEGFP,pEYFP,pECFP,pDsRED, pmCherry,pAcGFP,hrGFP,ZsGreen,pDsRed1-C1, pDsRed1-N1, pDsRED-Monomer-N1, pEYFP-C1, pEYFP-N1, pEGFP-1,pEGFP-C1, pEGFP-N1, pEGFP-N3, pcDNA-EGFP1,pECFP-N1,pECFP-C1, pAcGFP1-1 pDsRED2-ER, pDsRED2-Mito, pDsRED2-Nuc,pDsRED2-Peroxi , pEYFP-Golgi, pEYFP-Mem, pEYFP-ER , pEGFP-Actin, pECFP-ER, pIRES2-EGFP, pLEGFP-C1,pLEGFP-N1,pDsRED-Express-N1,pIRES2-DsRed,pIRES-AcGFP1,pmCherry-C1,N1 啟動(dòng)子增強(qiáng)子報(bào)告載體(Promega,帶有Luc熒光報(bào)告基因): pGL3-basic,pGL3-control,pGL3-enhancer,pGL3-promoter,pRL-TK,pRL-CMV,pRL-SV40, 信號(hào)通路報(bào)告載體(Clontech): pGAS-TA-Luc,pSTAT3-TA-Luc, pISRE-TA-Luc, pTA-Luc,pIκB-EGFP, pNFAT-TA-Luc,pCaspase3-sensor,pAP1(PMA)-Luc 信號(hào)通路報(bào)告載體(Promega):pGL4.26[luc2P/minP/Hygro],pGL4.29[luc2P/CRE/Hygro],pGL4.30[luc2P/NFAT-RE/Hygro],pGL4.75 信號(hào)通路報(bào)告載體(Stratagene): p53-Luc,pAP-1-Luc,pNF-κB-Luc,pSRE-Luc, pFA2-Elk1,pFC-MEKK,pFR-luc
昆蟲表達(dá)載體桿狀病毒表達(dá)載體系統(tǒng)pFastBac1,pFastBac1-Gus,pFastBacHT A pFastBacHT-CAT,pFastBacDual,pBlueBacHis2 A DH10Bac配套大腸桿菌,pMIB v5-His B ,pIZT/V5-His,
Taq,Pfu高表達(dá)載體菌株KOD, T4連接酶高表達(dá)質(zhì)粒載體,高產(chǎn)基因工程菌株、酶基因KOD,T4 kinase激酶,Tth反轉(zhuǎn)錄酶MMLV,Klentaq PCR酶,Cre酶,RNA inhibitor抑制酶,DL2000質(zhì)粒
BiFC雙分子熒光表達(dá)載體FRET系統(tǒng)載體pBiFC-VC155,pBiFC-VN173,pBiFC-bFosVC155,pBiFC-bFos(deltaZIP)VC155,pBiFC-bjun-VN173,pCE-BiFC-VN173,pCE-BiFC-VC155,
真菌表達(dá)載體黑曲霉米曲霉黃曲霉表達(dá)質(zhì)粒pBC-hygro,pAN7-1,pBARGPE1, 真菌ATMT農(nóng)桿菌介導(dǎo)轉(zhuǎn)化載體 pBHt1,pBIG2RHPH2-GFP-GUS等,
酵母單雜交三雜交載體,酵母雙雜交系統(tǒng)pGBKT7,pGADT7,pCL1,pGBKT7-53,pGADT7-T,pGBKT7-Lam,配套釀酒酵母AH109 酵母單雜交系統(tǒng)(Clontech):pHISi,pLacZi,pHis2,pHIS2.1 酵母三雜交系統(tǒng):pBridge, YBZ1菌株 Cytotrap Two-Hybrid System(Stratagene): pSos, pSos MAFB, pMyr
噬菌體展示系統(tǒng)載體1.噬菌體展示抗體scFv 質(zhì)粒:pCANTAB5E 宿主菌:TG1 輔助噬菌體:M13K07 可溶表達(dá)菌:HB2151 2.噬菌體展示抗體Fab 質(zhì)粒:pcomb3 宿主菌:XL1-Blue 輔助噬菌體:VCSM13
pEVOL,pBudCE4.1,pBudCE4.1-EGFP,Zeocin,pEGFP-C3,2,1,pmCherry-C1,-N1,pLOVE-miR302/367,pDsRed2-N1,pAcGFP1.1,TALE toolbox kit,pIRES-hyg3,pIRES-Neo3,pIRES-puro3,pSIREN-RetroQ-ZsGreen,GST 14-3-3,pDsRed-Monomer-N1,M50 super8x TOPFlash,pRK793,pCMV-tag5B,pCMV-tag2B,pCMV-N-HA,pUAST,pw-25,PAC-5.1-HISA,pSLIK-Neo,pCMV-myc-cyto,pCMV-myc-nuc,疫苗表達(dá)載體pVAX1,pAdEasy-1,pShuttle,pAdTrack,pAdTrack-CMV,-polyA,pShuttle-EGFP,BJ5183,pXC1,pBHGE3,pSUPER-retro-GFP-Neo,pORF-lacZ,pORF-TK,pCOX2-Luc,pcDNA3.1(-)-myc-His A,pcDNA3.1-p300,pINP, pOprE, pCR2.1 TOPO,pLP1,pLP2,pLP/VSVG,Stbl3,plenti6/V5-DEST,pLVX-shRNA1,2,pLVX-IRES,tdTomato,FUW-teto-hOCT4,hSOX2,hMYC,hKLF4,FUW-M2rtTA,pSecTag2 A,pEYFP-C1,pEGFP-N1,-C1,pcDNA3.1-V5-His A,B,C,pcDNA3.1-EGFP,pLEGFP-C1,α-MHC Promoter,Marc-145猴腎細(xì)胞,Sf9,BmN昆蟲家蠶細(xì)胞,酵母載體pYES2,pYES2.0,INVSc1,pYES3,pYES6,pRS41H,pRS303,304,305,306,pRS424,425,426,414,415,416,pY13,14,15,16,YBZ1,L40酵母菌,pESC-His,pYIP5,pHIS2,pHIS2.1,pHISi,pLacZi,酵母重組載體pUG6,pSH47,植物載體pCAMBIA1300-EGFP, pTCK303,pTRV1,pTRV2,pFGC5941,pUN1301,pSN1301,C58C1,R1601,AGL1農(nóng)桿菌感受態(tài),原核載體pQE3,pQE30,31,32,pQE60,61,62,M15,DH10Bac,Origami(B)DE3,XL1-Blue,Rosetta gamiB(DE3)pLysS,TKB1,ER2566,DB3.1,JM105,*0,DH5a,DH5alpha lambda pir,C43(DE3)菌株感受態(tài),pTrcHis A,B,C,pTWIN1,pTYB1,pTYB11,pACYC184,pMAL-c5x,c2x,p5x,c4x,pBV220,221,222,pCold I,II,III,TF,pET22a,pET29a,pET28a,pET39b,pET41a,pET42a,p15A,pET-sumo,pBADHis A,B,C,pBAD24,34,pETDuet-1,pRSFDuet-1,pCDFDuet-1,pACYCDuet-1,原核熒光pQBI63,pMP2444,鏈球菌表達(dá)、敲除載體pKC1139、pOJ260、pWHM3,pSET152,pVA981,多宿主載體pGOAL19,pBBR1MCS-2,-5,-6,pDSK519,pLA2917,pRK2013,pVLT33,pMAD,RN4220金葡菌株,真菌載體pBARGPE1,pAN7-1,pBHt1,pBIG2RHPH2-GFP-GUS,乳酸菌載體MG1363,pNZ8148,NZ9000,pNZ9530,pMG36e,枯草芽孢桿菌載體pHT43,pWB980,pHT300PLK,pMA5,pUTminiTn5,巨大芽孢桿菌表達(dá)載體pHIS1525,WH320菌,谷氨酸棒狀桿菌表達(dá)載體pEC-XK99E,敲除載體pk18mobsacB,
pET載體
3a, 3d
3c-sumo
5b
11a A+
12a A+
15b A+
16b A+
17b A+
19b A+
20b A+
21a,21b,21d A+
22b A+
23a,23b A+
23a-tev
24a K+
25b K+
26b K+
27b K+
28a,28b,28c K+
28a-sumo
28a-p53
29a K+
30a ,30c K+
31b A+
32a A+
32a pelB A+
35b K+
39b K+
40b K+
41a,41b k+
42a,42b K+
43b A+
43.1a A+
44a A+
48b K+
49b K+
50b K+
pETduet-1
pACYCduet-1
pCDFduet-1
pRSFduet-1
pColAduet-1
pET His
pET DsbA
pET GST
pET Trx
pQE載體
PQE2
pQE9
pQE30,31,32,
pQE30UA
pQE30LacIq
pQE40L
pQE60
pQE70
pQE80L
其他原核表達(dá)載體
PBBR1 MCS Cm+
PBBR1 MCS-2 K+
PBBR1 MCS-3 Tet+
PBBR1 MCS-4 A+
PBBR1 MCS-5 慶大霉素
pMal c2x,p2x,5x,5E
pBV220,221,222
pTrc99a,pTrcCKS,pTrcHis A,B,C
pBAD24,33,43,pBADHis A,B,C,pBADmycHisA
pEZZ18
pThio HisA A+
pGEX4T-1,4T-2,4T-3,5X-1,6p-1,2t,3x
pColdI
pColdII
pColdIII
pColdTF
pTwin1
pRsetA,B,C
PLLP-STQ
pINⅢ-ompA
pkk223-3
pSE380
pJLA502
pMBP-C,pMBP-p
pTYB11,pCYB1
Origene-N-His GST A+
pPin point CAT A+
pPin point xa1 A+
pPin point xa2 A+
pPin point xa3 A+
pCW ori
、
常見克隆載體
puc18,puc19,puc118,puc119
pBlue Script SK+,
pBlue ScriptⅡSK+
pEGM-3ZF(+) A+
pEGM-11ZF(+) A+
pACYC184
pBR322
基因敲除
pKD3,pKD4,pKD46,pcp20
pK18mobSacB
海腎熒光素酶報(bào)告基因
pGL3basic,promotor,enhancer,control
pGL3 CM
pGL4.29,4.30
畢赤酵母表達(dá)載體
pPIC9
pPIC9k
pPIC9k-1
pPIC9k-His
pPIC3.5
pPIC3.5k
pAO815
pHIL-S1
pPICZalphaA,B,C
pPICZalpha D
pPICZalpha E
pPICZalpha GA,GB
pPICZalpha FC
pPICZA,B
pGAPZalphaA,B,C
pMETalphaA,B,C
pFLD-cat A+
pFLD-cat Z+
pFLD1 Z+
畢赤酵母宿主菌
JC220
JC308
GS200
GS190
GS115
KM71
KM71H
Y1991
SMD1168
SMD1168H
X33
釀酒酵母表達(dá)載體
pYES2,pYES2-flag
pYES2NTA,NTB,NTC
pYES3CT
pYIP5,pYRP7,pADH2
pRSH41,pDC315,pDF15,pUG6
pESC-His,BFD14,BFD15,pDNR1,p334,pDR195
pRS316,pRS316HA
YEplac112,YCplac33,YCplac22-EGFP,
YEplac195,YEpLac181,YIP211
pYX212,pYX212-VHB
釀酒酵母宿主菌
INSV-
YPH499
YM4271
BY4741
W303-1A
真核哺乳動(dòng)物熒光載體
pEGFP N1,N2,N3
Origene GFP
pLEGFP N1
pEGFP C1,C3
AIF-EGFP N1
pECFP N1,C1
pEYFP N1,C1
pEYFP-golgi
pEYFPmem
pBFPN1
FIV-BFP
pDsred MonomerN1
pDsRed1 mito
pDsRed2 mito
pDsRed N1,C1
pDsRed2 N1,C1
pDsRed2-ER
mRFP
真核哺乳動(dòng)物載體
pIRES
pIRES2-EGFP
pADeasy-1
pADeasy-1(Bj5183)
pAdtrack
pAdtrack CMV
pShuttle
pShuttle CMV
pRL CMV
pCMV-HA
pVAX1
pVAST
pCI
pCI neo+
pCMV-myc
pflag-CMV2
pcDNA3.1 +
pcDNA3.1 zeo+
pcDNA3.1 neo+
pcDNA3.1 MycHisA
pcDNA3.1 MycHisB
pcDNA3.1-HBV genome
pcDNA3-mRFP
Hsp70-pcDNA3.1
pcDNA 4 Max B
psuper GFP neo
psuper Retro GFP neo
pSilencer4.1CMV puro+
pSilencer4.1CM neo+
psuper GFP neo
psuper retro GFP neo
pdisplay
pBud CE4.1
ptet on, ptet off
pRL-tk,pRL-CMV
植物表達(dá)載體
pcambia1301,1302,1303,1304,1305.2
2300,2301
3300
pcambia1301(EHA105)
pcambia1381Z,1391Z
PBI121,221
PBI221-GFP
植物農(nóng)桿菌宿主
EHA105,LBA4404
植物RNAi載體
PHANNIBAL,PKANNIBAL
啟動(dòng)子檢測(cè)
pEGFP-1
pkk232-8
分子伴侶:
PGKJE8,PGro7,pTf16,pKJE7,pGTf2
酵母雙雜交
pGADT7,pGBKT7,pGADT7-T,pGBKlam,pGBK-53,pCL1
AH109,Y187
酵母單雜交
pGAD53m,p53-blue,p53-His,pLacZ,pHis,pHisi-1,
哺乳動(dòng)物雙雜交
pBIND-GFP,pBIND-ID,pGAD424,pGBT9,pG5lac,
大腸桿菌宿主菌
JM101,105,109,110
HB101,K802,C600,TH1,AM1,W3110,DH10bac
Y1089,Y1090,BL21,BL21(DE3),MG1655
BL21(DE3)plysS,BL21AI,BL21SI,BL21codon plus RIPL,BL21 trxB (DE3),BL21 star
DH5α,DH5αλpir,JM109,JM109(DE3),*0,*0F,,K12,DB3.1,XL1-blue,XL-10gold,BM25.8,SG1117,Turbo,TG1,TB1,C41(DE3),C43(DE3),Turner(DE3),M15,E2566,C2566,ER2529,JF1125,Bj5183,Stbl3,Stbl4
HMS174(DE3), HMS174(DE3)plysS, AD494(DE3)
Novablue(DE3),Rosettalblue(DE3)
Rosetta(DE3),Rosetta(DE5),Rosetta(DE3)plysS,
Orgami(DE3),OrgamiB(DE3),Orgami(DE3)plysS
Rosetta-gami2(DE3)plysS,Rosetta-gami(DE3),
Rosetta-gamiB(DE3),Rosetta-gamiB(DE3)plysS, Rosetta-gami(DE3)placI
枯草表達(dá)系統(tǒng)pMA5,pHCMC05,pGFP22,pGFP315,pHP13,pHP13-43,pHY-43,pWB980,pAOX1
枯草宿主菌:BS168,DB403,BCL,1A75,WB600,WB800N
昆蟲表達(dá)系統(tǒng):pfastBacI,pfastII,pfastIII,pfastBacHTA,pfastBacHTB,pfastBacHTC,pfastBacdual
巨大芽孢桿菌系統(tǒng):pHis1525
巨大宿主菌:YYBM1,WH320
乳酸球菌表達(dá)系統(tǒng) pMG36e,NZ9000,MG1363
乳酸酵母表達(dá)系統(tǒng) pKLAC1,GG799
白色念珠菌系統(tǒng)pCR4,pCR4-GFP,p5921
釀酒酵母表面展示系統(tǒng):pYD1,EBY100
標(biāo)準(zhǔn)微生物菌株
ATCC27853 銅綠假單胞菌
ATCC29212 糞鏈球菌
ATCC29213 金黃色葡萄球菌
ATCC35218 大腸桿菌
ATCC 35098四聯(lián)球菌
NRRL Y-12968熱帶假絲酵母
NRRL Y-2021德巴利酵母
NRRL Y-1603畢赤氏巴斯德酵母
DSM3586野油菜黃