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中級(jí)會(huì)員·13年
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可提供近萬(wàn)種質(zhì)粒載體、菌株、基因和細(xì)胞株,并可提供實(shí)驗(yàn)技術(shù)外包服務(wù),如基因克隆、質(zhì)粒構(gòu)建、蛋白原核、真核表達(dá)及純,pQE 載體,原核表達(dá)載體,克隆載體 ,畢赤酵母表達(dá)載體,畢赤酵母宿主菌,釀酒酵母表達(dá)載體 ,釀酒酵母宿主菌,真菌表達(dá)載體,真核哺乳動(dòng)物熒光載體,真核哺乳動(dòng)物載體,植物表達(dá)載體,大腸桿菌宿主菌,枯草宿主菌
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下面所有質(zhì)粒和菌株,均為本公司實(shí)驗(yàn)室親自驗(yàn)證過(guò),可以提供,載體來(lái)源于promega,addgene,clontech,novagen,invitrogen可提供質(zhì)粒圖譜,可提供近萬(wàn)種質(zhì)粒載體、菌株、基因和細(xì)胞株,并可提供實(shí)驗(yàn)技術(shù)外包服務(wù),如基因克隆、質(zhì)粒構(gòu)建、蛋白原核、真核表達(dá)及純,pQE 載體,原核表達(dá)載體,克隆載體 ,畢赤酵母表達(dá)載體,畢赤酵母宿主菌,釀酒酵母表達(dá)載體 ,釀酒酵母宿主菌,真菌表達(dá)載體,真核哺乳動(dòng)物熒光載體,真核哺乳動(dòng)物載體,植物表達(dá)載體,大腸桿菌宿主菌,枯草宿主菌

 

釀酒酵母表達(dá)載體

 

pYES2,pYES2/NT,pYES2/CT,pYES3,pYES6, pYCplac22-GFP,酵母載體pAUR123,pRS303TEF,pRS304, pRS305,pRS306,pY13TEF,pY14TEFpY15TEFpY16TEF,酵母基因重組表達(dá)載體pUG6, pSH47,酵母單雜載體pHISi,pLacZi,pHIS2, pGAD424, 酵母雙雜交系統(tǒng):釀酒酵母Y187,釀酒酵母AH109;質(zhì)粒pGADT7,pGBKT7;對(duì)照質(zhì)粒pGBKT7-53pGBKT7-lampGADT7-TPCL1,釀酒酵母菌株INVSc1,YM4271, AH109,Y187,Y190, 畢赤酵母表達(dá)載體pPIC9K,pPIC9K-His,pPIC3.5K,pPICZalphaA,B,C,pPICZA,B,C,pGAPZαA,pAO815,pPIC9k-His,pHIL-S1,pPink hc,配套畢赤酵母Pichiapink,畢赤酵母宿主X33KM71KM71HGS115

 

 

 

原核表達(dá)載體pQE30,31,32,40,60,61,62,等原核表達(dá)載體,包括pET系列,pET-GSTpGEX系列GST標(biāo)簽pMAL系列pMAL-c2x,-c4x,-c4e,-c5x,-p5x,pBAD,pBADHis,pBADmycHis系列,pQE系列,pTrc99a,pTrcHis系列,pBV220,221,222,pTXB系列,pLLP-ompA,pIN-III-ompA(分泌型表達(dá)系列),pQBI63(原核表達(dá)帶熒光)pET3a, pET 3d, pET 11a, pET 12a, pET 14b, pET 15b, pET 16b, pET 17b, pET 19b, pET 20b, pET 21a,b,d, pET 22b, pET 23a, pET 23b, pET 24a,b, pET 25b, pET 26b, pET 27b, pET 28a,b, pET 29a, pET 30a, pET 31b, pET 32a, pET 35b, pET 38b, pET 39b, pET 40b, pET 41a,b pET 42a, pET 43.1a,b pET 44a, pET 49b pET302,303 pET His,pET Dsb,pET GST,pET Trx pQE2, pQE9 pQE30,31,32, pQE 40 pQE70 pQE80L pQETirs system pRSET-A pRSET-B pRSET-C pGEX4T-1,-2,-3,5x-1,6p-1,6p-2,2tk,3c pBV220,221,222 pTrcHisA,B,C pBAD24,34,43 pBAD HisA,B,C pPinPoint-Xa1,Xa2,Xa3 pMALc2x, p2x pBV220 pGEM Ex1, pGEM7ZF(+), pTrc99A, pTwin1, pEZZ18 pkk232-8,pkk 233-3,pACYC184,pBR322,pUC119 pTYB1,pTYB2,pTYB4,pTYB11 pBlueScript SK(+),pBlueScript SK(-) pLLP ompA, pINIIIompA, pMBP-P ,pMBP-C, 大腸桿菌冷激質(zhì)粒: pColdI pColdII pColdIII pColdTF 原核共表達(dá)質(zhì)粒:pACYCduet-1,pETduet-1,pCDFduet-1pRSFduet-1 Takara公司大腸桿菌分子伴侶: pG-KJE8 pGro7 pKJE7 pGTf2 pTf16 大腸桿菌宿主細(xì)胞: DH5a JM101 JM103 JM105 JM107 JM109 JM110 *0 *0F BL21(DE3) HB101 ER2529 E2566 C2566 MG1655 XL-10gold XL blue M15 JF1125 K802 SG1117 BL21(AI) BL21(DE3)plysS TG1 TB1 DH5a(pir) Tuner(DE3) Bl21 codonplusRIPL Novablue(DE3) Rosetta Rosetta(DE3) Rosetta(DE3)plys Rosetta-gami(DE3) Rosetta-gamiB(DE3), Rosetta-gamiB(DE3)plysS Orgami(DE3) OrgamiB(DE3) HMS174(DE3)

 

 

 

植物表達(dá)/RNAi載體農(nóng)桿菌pBI121,pBI121-GFP,pBI101,pBI221,pSN1301, pUN1301,pRTL2 , pRTL2-GFP , pRTL2-CFP, pRTL2-RFP , pRTL2-YFP,pCAMBIA 1300, 1301, 1302,1303,1304,1305, 1381Z,1391Z,2300, 2301,3300,3301,pCAMBIA super1300,pCAMBIA super1300-GFP,pPZP212,pPZP2121,pPZP212-GFP,pGDG,RNAi載體pART27,pHANNIBAL,pKANNIBAL, pFGC5941,pTCK303, pTRV1,pTRV2, T-DNA插入載體隨機(jī)突變體庫(kù))pSKI015,pSKI074,真菌ATMT載體pBIG2RHPH2-GUS-GFP,pBHt1

 

 

 

枯草芽孢桿菌表達(dá)載體pWB980,pHT43,pHP13,pHP43, pBE2,pMUTIN4,pUB110,pE194,pMA5, pMK3,pMK4,pHT304,pHY300PLK, pBest502,pDG1363,pSG1154,pAX01, pSAS144,pDL,pDG148-stu,pDG641, pAL12,pUCX05-bgaB,pHT01 配套菌株BS 168,WB600,WB800,WB700, WB800N,1012,FZB42,1A747,廣宿主質(zhì)粒pVLT33

 

 

 

RNAi基因沉默干擾敲除載體pSilencer1.0pSilencer 2.1-U6 hygro pSilencer 3.1-H1 hygropSilencer 3.1-H1 neo pSilencer 4.1-CMV neo pSilencer 4.1-CMV puro pMIR-REPORT Luciferase RNAi載體(oligoengine) pSuper-puro RNAi逆轉(zhuǎn)錄病毒載體(clontech): RNAi-Ready pSIREN-Retro Q RNAi-Ready pSIREN-RetroQ-ZsGreen(Luciferase shRNA Annealed Oligonucleotide) RNAi慢病毒載體(addgene): pLKO.1

 

 

 

基因cDNA文庫(kù)ORF表達(dá)克隆質(zhì)粒,

 

擁有目前中國(guó)zui大的cDNA克隆庫(kù)、覆蓋10萬(wàn)人、鼠全長(zhǎng)基因的cDNA克隆和超過(guò)25,000個(gè)ORF克隆表達(dá)載體。

 

 

 

哺乳動(dòng)物表達(dá)載體pcDNA3.1+/-,pcDNA4/HisMax BpSecTag2 ApVAX1pBudCE4.1,pTracer CMV2pcDNA3.1(-)/myc-His A pcDNA6-Myc/His BpCEP4 pIRESpIRESneopIRES hyg3pCMV-mycpCMV-HApIRES-puro3pIRES-neo3,pCAGGS哺乳動(dòng)物雙雜交系統(tǒng)pACTpBINDpACT-MyoDpBIND-IdpG5luc,pCMV-BD, pCMV-AD, pBD-p53, pFR-luc,Cytotrap Two-Hybrid System:pSos, pSos MAFB, pMyr蛻皮激素誘導(dǎo)系統(tǒng)pIND, pVgRxR,LacSwith II哺乳動(dòng)物誘導(dǎo)表達(dá)系統(tǒng):pOPRSVI pOPI3CATpCMVLacI,GeneSwitch System:pSwitch哺乳動(dòng)物表面展示系統(tǒng):pDisplay, 四環(huán)素調(diào)控系統(tǒng)(Invitrogen)pcDNA4/TO/Myc-His ApcDNA4/TO/Myc-His BpcDNA4/TO/Myc-His CpcDNA4/TO/Myc-His/LacZpcDNA6/TR四環(huán)素調(diào)控系統(tǒng)(Clontech)pTet-OnpTet-OffpTRE2,pRevTREpRevTet-OnpRevTet-off信號(hào)通路報(bào)告載體:pGAS-TA-LucpSTAT3-TA-Luc, pISRE-TA-Luc, pTA-LucpIκB-EGFPpNFAT-TA-LucpCaspase3-sensorpAP1(PMA)-Luc;pGL4.26[luc2P/minP/Hygro],pGL4.29[luc2P/CRE/Hygro],pGL4.30[luc2P/NFAT-RE/Hygro]pGL4.75;p53-LucpAP-1-Luc pNF-κB-LucpSRE-LucpFA2-Elk1pFC-MEKKpFR-luc,Gateway系統(tǒng)(invitrogen)pcDNA6.2-GWEmGFP-miR negative, pLenti 6/TR,pcDNA 6.2-GW EmGFP-miR

 

 

 

乳酸菌表達(dá)載體及各種乳酸菌乳酸桿菌菌株,pNZ8148,pLEISS,pMG36e,pBBR1MCS-5,pBBR1MCS-6,pRV610,pLEM415,pHY300PLK,分泌型乳酸菌表達(dá)載體pVE5523pPG611.1,pPG612.1等和乳酸桿菌菌株宿主菌NZ9000,MG1363,Lactobacillus casei 1.539,Lactobacillus casei,acidophilus NCFM,1.2,Lactobacillus sakei 23K,L.plantarum,L.rhamnosus GG,B.coagulans,Bifidobacterium bifidum,Bifidobacterium infantis,Lactococcus lactis M17,1663,Lactobacillus reuterii

 

 

 

廣宿主表達(dá)載體鏈球菌表達(dá)敲除載體假單胞菌表達(dá)載體pVLT33,pBBR1MCS-2,3,4,5,6, pJRD215,pJN105,pME6032,Cos載體pLAFR3,pMP2444(GFP), pHY300PLK,pRT102,pRL1063a, 轉(zhuǎn)座子載體pUT-miniTn5,pMGS100, pWHM10,pKC1139,pSET152, pOJ260,pPG611.1,pPG612.1

 

 

 

腺病毒載體/慢病毒,逆轉(zhuǎn)錄病毒表達(dá)載體及包裝包膜質(zhì)粒,腺病毒系統(tǒng)(Stratagene): pAdEasy-1,pShuttle-CMV,pShuttle,pAdTrack, pAdTrack-CMV, pShuttle-IRES-hrGFP-1pShuttle-IRES-hrGFP-2pShuttle-CMV-lacZ, pShuttle-CMV-EGFP-C,pXC1, pBHGE3, 配套大腸桿菌BJ5183,293,293T cell line 腺相關(guān)病毒系統(tǒng)(Stratagene) pAAV-MCSpAAV-RCpHelperpAAV-LacZpAAV-IRES-hrGFPpCMV-MCS 慢病毒載體:pLVX-DsRed-Monomer-N1,pLVX-IRES-ZsGreen1,pLVX-AcGFP1-N1,Lenti6/v5-EDST-EGFP,pWPXL, FUGW,pLentilox 3.7,RNAi-Ready pSIREN-Retro Q, RNAi-Ready pSIREN-Retro Q-ZsGreen, pSUPER.Retro-GFP/Neo,pSUPER-Retro-Neo, pSUPER.Retro-puro,PLNCX PLNCX2 pMSCV-HYG pMSCV-neo pMSCV-puro pLEGFP-C1 pLOX-CW-CRE pLOX-GFP-IRES-TK pRetroX-IRES-DsRedExpress, pLVX-IRES-mCherry質(zhì)粒載體。 病毒包裝:二質(zhì)粒包裝系統(tǒng)一: psPAX2 pMD2.G 二質(zhì)粒系統(tǒng)二: pCMV-dR8.2 dvpr pCMV-VSVG 三質(zhì)粒包裝系統(tǒng)具有更高的包裝效率: pMDLg/pRRE pRSV-Rev pMD2.G Invitrogen包裝系統(tǒng): PLP1 PLP2 PLP/VSVG

 

 

 

基因重組載體敲除載體自殺質(zhì)粒RED重組系統(tǒng)載體pKD3,pKD13,pKD4,pCP20,pKD46, pCVD442,pk18mobsacB,p2NIL, pMAD(革蘭氏陽(yáng)性菌基因敲除pBT2,RN4220,pKC1139,pOJ260, pJQ200SK,pSET152,pWHM10, pk18mobsacB, pGOAL17,pGOAL19,基因打靶載體PL253PL451PL452PL453,配套菌株EL250,EL350,pMLM290,292, KJBAC1,pST1374

 

 

 

熒光蛋白表達(dá)載體熒光定位質(zhì)粒報(bào)告基因載體pEGFP,pEYFP,pECFP,pDsRED, pmCherry,pAcGFP,hrGFP,ZsGreenpDsRed1-C1, pDsRed1-N1, pDsRED-Monomer-N1, pEYFP-C1, pEYFP-N1, pEGFP-1pEGFP-C1, pEGFP-N1, pEGFP-N3, pcDNA-EGFP1,pECFP-N1,pECFP-C1, pAcGFP1-1 pDsRED2-ER, pDsRED2-Mito, pDsRED2-NucpDsRED2-Peroxi , pEYFP-Golgi, pEYFP-Mem, pEYFP-ER , pEGFP-Actin, pECFP-ER, pIRES2-EGFP, pLEGFP-C1pLEGFP-N1pDsRED-Express-N1,pIRES2-DsRed,pIRES-AcGFP1,pmCherry-C1,N1 啟動(dòng)子增強(qiáng)子報(bào)告載體(Promega,帶有Luc熒光報(bào)告基因 pGL3-basicpGL3-controlpGL3-enhancerpGL3-promoterpRL-TKpRL-CMVpRL-SV40, 信號(hào)通路報(bào)告載體(Clontech) pGAS-TA-LucpSTAT3-TA-Luc, pISRE-TA-Luc, pTA-LucpIκB-EGFP pNFAT-TA-LucpCaspase3-sensorpAP1(PMA)-Luc 信號(hào)通路報(bào)告載體(Promega)pGL4.26[luc2P/minP/Hygro],pGL4.29[luc2P/CRE/Hygro],pGL4.30[luc2P/NFAT-RE/Hygro]pGL4.75 信號(hào)通路報(bào)告載體(Stratagene) p53-LucpAP-1-LucpNF-κB-LucpSRE-Luc pFA2-Elk1pFC-MEKKpFR-luc

 

 

 

昆蟲(chóng)表達(dá)載體桿狀病毒表達(dá)載體系統(tǒng)pFastBac1,pFastBac1-Gus,pFastBacHT A pFastBacHT-CAT,pFastBacDual,pBlueBacHis2 A DH10Bac配套大腸桿菌,pMIB v5-His B ,pIZT/V5-His

 

 

 

Taq,Pfu高表達(dá)載體菌株KOD, T4連接酶高表達(dá)質(zhì)粒載體,高產(chǎn)基因工程菌株、酶基因KOD,T4 kinase激酶,Tth反轉(zhuǎn)錄酶MMLVKlentaq PCR酶,Cre酶,RNA inhibitor抑制酶,DL2000質(zhì)粒

 

 

 

BiFC雙分子熒光表達(dá)載體FRET系統(tǒng)載體pBiFC-VC155pBiFC-VN173pBiFC-bFosVC155pBiFC-bFos(deltaZIP)VC155pBiFC-bjun-VN173pCE-BiFC-VN173pCE-BiFC-VC155

 

 

 

真菌表達(dá)載體黑曲霉米曲霉黃曲霉表達(dá)質(zhì)粒pBC-hygro,pAN7-1,pBARGPE1, 真菌ATMT農(nóng)桿菌介導(dǎo)轉(zhuǎn)化載體 pBHt1,pBIG2RHPH2-GFP-GUS等,

 

 

 

酵母單雜交三雜交載體,酵母雙雜交系統(tǒng)pGBKT7pGADT7pCL1pGBKT7-53pGADT7-TpGBKT7-Lam,配套釀酒酵母AH109 酵母單雜交系統(tǒng)(Clontech):pHISi,pLacZi,pHis2,pHIS2.1 酵母三雜交系統(tǒng):pBridge, YBZ1菌株 Cytotrap Two-Hybrid System(Stratagene): pSos, pSos MAFB, pMyr

 

 

 

噬菌體展示系統(tǒng)載體1.噬菌體展示抗體scFv 質(zhì)粒:pCANTAB5E 宿主菌:TG1 輔助噬菌體:M13K07 可溶表達(dá)菌:HB2151 2.噬菌體展示抗體Fab 質(zhì)粒:pcomb3 宿主菌:XL1-Blue 輔助噬菌體:VCSM13

 

pEVOLpBudCE4.1,pBudCE4.1-EGFP,Zeocin,pEGFP-C3,2,1,pmCherry-C1,-N1,pLOVE-miR302/367,pDsRed2-N1,pAcGFP1.1,TALE toolbox kit,pIRES-hyg3,pIRES-Neo3,pIRES-puro3,pSIREN-RetroQ-ZsGreen,GST 14-3-3,pDsRed-Monomer-N1,M50 super8x TOPFlash,pRK793,pCMV-tag5B,pCMV-tag2BpCMV-N-HApUAST,pw-25,PAC-5.1-HISApSLIK-Neo,pCMV-myc-cyto,pCMV-myc-nuc,疫苗表達(dá)載體pVAX1,pAdEasy-1,pShuttle,pAdTrack,pAdTrack-CMV,-polyA,pShuttle-EGFP,BJ5183,pXC1,pBHGE3,pSUPER-retro-GFP-Neo,pORF-lacZpORF-TKpCOX2-Luc,pcDNA3.1(-)-myc-His A,pcDNA3.1-p300,pINP, pOprE, pCR2.1 TOPO,pLP1,pLP2,pLP/VSVG,Stbl3,plenti6/V5-DEST,pLVX-shRNA1,2,pLVX-IRES,tdTomato,FUW-teto-hOCT4,hSOX2,hMYC,hKLF4,FUW-M2rtTA,pSecTag2 A,pEYFP-C1,pEGFP-N1,-C1,pcDNA3.1-V5-His A,B,C,pcDNA3.1-EGFP,pLEGFP-C1,α-MHC Promoter,Marc-145猴腎細(xì)胞,Sf9BmN昆蟲(chóng)家蠶細(xì)胞,酵母載體pYES2,pYES2.0,INVSc1,pYES3,pYES6,pRS41H,pRS303,304,305306,pRS424,425,426,414,415,416,pY13,14,15,16,YBZ1,L40酵母菌,pESC-His,pYIP5,pHIS2,pHIS2.1,pHISi,pLacZi,酵母重組載體pUG6,pSH47,植物載體pCAMBIA1300-EGFP, pTCK303,pTRV1,pTRV2,pFGC5941,pUN1301,pSN1301,C58C1R1601,AGL1農(nóng)桿菌感受態(tài),原核載體pQE3,pQE30,31,32,pQE60,61,62,M15,DH10Bac,Origami(B)DE3,XL1-Blue,Rosetta gamiB(DE3)pLysS,TKB1,ER2566DB3.1JM105,*0,DH5a,DH5alpha lambda pir,C43(DE3)菌株感受態(tài),pTrcHis A,B,C,pTWIN1,pTYB1,pTYB11,pACYC184,pMAL-c5x,c2x,p5x,c4x,pBV220,221,222,pCold I,II,III,TF,pET22a,pET29a,pET28a,pET39b,pET41a,pET42a,p15A,pET-sumo,pBADHis A,B,C,pBAD24,34,pETDuet-1,pRSFDuet-1,pCDFDuet-1,pACYCDuet-1,原核熒光pQBI63,pMP2444,鏈球菌表達(dá)、敲除載體pKC1139pOJ260pWHM3pSET152pVA981,多宿主載體pGOAL19,pBBR1MCS-2,-5,-6,pDSK519,pLA2917pRK2013pVLT33,pMAD,RN4220金葡菌株,真菌載體pBARGPE1,pAN7-1,pBHt1,pBIG2RHPH2-GFP-GUS,乳酸菌載體MG1363,pNZ8148,NZ9000,pNZ9530,pMG36e,枯草芽孢桿菌載體pHT43,pWB980,pHT300PLK,pMA5,pUTminiTn5,巨大芽孢桿菌表達(dá)載體pHIS1525,WH320,谷氨酸棒狀桿菌表達(dá)載體pEC-XK99E,敲除載體pk18mobsacB,

pET載體

3a, 3d

3c-sumo

5b

11a A+

 

12a A+

15b A+

16b A+

17b A+

19b A+

20b A+

21a,21b,21d A+

22b A+

23a,23b A+

23a-tev

24a K+

25b K+

26b K+

27b K+

28a,28b,28c K+

28a-sumo

28a-p53

29a K+

30a ,30c K+

31b A+

32a A+

32a pelB A+

35b K+

39b K+

40b K+

41a,41b k+

42a,42b K+

43b A+

43.1a A+

44a A+

48b K+

49b K+

50b K+

pETduet-1

pACYCduet-1

pCDFduet-1

pRSFduet-1

pColAduet-1

pET His

pET DsbA

pET GST

pET Trx

pQE載體

PQE2

pQE9

pQE30,31,32,

pQE30UA

pQE30LacIq

pQE40L

pQE60

pQE70

pQE80L

 

其他原核表達(dá)載體

PBBR1 MCS Cm+

PBBR1 MCS-2 K+

PBBR1 MCS-3 Tet+

PBBR1 MCS-4 A+

PBBR1 MCS-5 慶大霉素

 

 

pMal c2x,p2x,5x,5E

pBV220,221,222

pTrc99a,pTrcCKSpTrcHis A,B,C

pBAD24,33,43,pBADHis A,B,C,pBADmycHisA

pEZZ18

pThio HisA A+

pGEX4T-1,4T-24T-35X-1,6p-1,2t,3x

pColdI

pColdII

pColdIII

pColdTF

pTwin1

pRsetA,B,C

PLLP-STQ

pIN-ompA

pkk223-3

pSE380

pJLA502

pMBP-CpMBP-p

pTYB11,pCYB1

Origene-N-His GST A+

pPin point CAT A+

pPin point xa1 A+

pPin point xa2 A+

pPin point xa3 A+

pCW ori

 

 

常見(jiàn)克隆載體

puc18,puc19puc118puc119

pBlue Script SK+,

pBlue ScriptSK+

pEGM-3ZF(+) A+

pEGM-11ZF(+) A+

pACYC184

pBR322

 

基因敲除

pKD3,pKD4pKD46,pcp20

pK18mobSacB

 

海腎熒光素酶報(bào)告基因

pGL3basicpromotorenhancercontrol

pGL3 CM

pGL4.29,4.30

 

 

畢赤酵母表達(dá)載體

pPIC9

pPIC9k

pPIC9k-1

pPIC9k-His

pPIC3.5

pPIC3.5k

pAO815

pHIL-S1

pPICZalphaABC

pPICZalpha D

pPICZalpha E

pPICZalpha GA,GB

pPICZalpha FC

pPICZAB

pGAPZalphaABC

pMETalphaA,B,C

pFLD-cat A+

pFLD-cat Z+

pFLD1 Z+

 

畢赤酵母宿主菌

JC220

JC308

GS200

GS190

GS115

KM71

KM71H

Y1991

SMD1168

SMD1168H

X33

 

 

 

釀酒酵母表達(dá)載體

pYES2,pYES2-flag

pYES2NTA,NTB,NTC

pYES3CT

pYIP5,pYRP7,pADH2

pRSH41,pDC315,pDF15,pUG6

pESC-His,BFD14,BFD15,pDNR1,p334,pDR195

pRS316,pRS316HA

YEplac112,YCplac33,YCplac22-EGFP,

YEplac195YEpLac181,YIP211

pYX212pYX212-VHB

 

釀酒酵母宿主菌

INSV-

YPH499

YM4271

BY4741

W303-1A

 

真核哺乳動(dòng)物熒光載體

pEGFP N1,N2N3

Origene GFP

pLEGFP N1

pEGFP C1C3

AIF-EGFP N1

pECFP N1,C1

pEYFP N1,C1

pEYFP-golgi

pEYFPmem

pBFPN1

FIV-BFP

pDsred MonomerN1

pDsRed1 mito

pDsRed2 mito

pDsRed N1C1

pDsRed2 N1,C1

pDsRed2-ER

mRFP

 

真核哺乳動(dòng)物載體

pIRES

pIRES2-EGFP

pADeasy-1

pADeasy-1(Bj5183)

pAdtrack

pAdtrack CMV

pShuttle

pShuttle CMV

pRL CMV

pCMV-HA

pVAX1

pVAST

pCI

pCI neo+

pCMV-myc

pflag-CMV2

pcDNA3.1 +

pcDNA3.1 zeo+

pcDNA3.1 neo+

pcDNA3.1 MycHisA

pcDNA3.1 MycHisB

pcDNA3.1-HBV genome

pcDNA3-mRFP

Hsp70-pcDNA3.1

pcDNA 4 Max B

psuper GFP neo

psuper Retro GFP neo

pSilencer4.1CMV puro+

pSilencer4.1CM neo+

psuper GFP neo

psuper retro GFP neo

pdisplay

pBud CE4.1

ptet on, ptet off

pRL-tkpRL-CMV

 

 

 

植物表達(dá)載體

pcambia1301,1302,1303,1304,1305.2

       2300,2301

       3300

pcambia1301(EHA105)

pcambia1381Z,1391Z

PBI121221

PBI221-GFP

 

植物農(nóng)桿菌宿主

EHA105LBA4404

 

植物RNAi載體

PHANNIBALPKANNIBAL

 

啟動(dòng)子檢測(cè)

pEGFP-1

pkk232-8

 

分子伴侶:

PGKJE8PGro7pTf16pKJE7pGTf2

 

酵母雙雜交

pGADT7,pGBKT7,pGADT7-T,pGBKlam,pGBK-53,pCL1

AH109,Y187

 

酵母單雜交

pGAD53m,p53-blue,p53-His,pLacZ,pHispHisi-1

 

 

哺乳動(dòng)物雙雜交

pBIND-GFPpBIND-ID,pGAD424pGBT9pG5lac,

 

 

大腸桿菌宿主菌

JM101,105,109,110

HB101,K802,C600,TH1,AM1,W3110,DH10bac

Y1089,Y1090,BL21,BL21(DE3),MG1655

BL21(DE3)plysSBL21AIBL21SIBL21codon plus RIPLBL21 trxB (DE3)BL21 star

DH5α,DH5αλpirJM109JM109(DE3)*0*0F,K12DB3.1XL1-blueXL-10goldBM25.8SG1117TurboTG1TB1C41(DE3)C43(DE3),Turner(DE3),M15E2566C2566ER2529JF1125Bj5183,Stbl3,Stbl4

HMS174(DE3), HMS174(DE3)plysS, AD494(DE3)

Novablue(DE3),Rosettalblue(DE3)

Rosetta(DE3),Rosetta(DE5),Rosetta(DE3)plysS,

Orgami(DE3),OrgamiB(DE3),Orgami(DE3)plysS

Rosetta-gami2(DE3)plysSRosetta-gami(DE3),

Rosetta-gamiB(DE3),Rosetta-gamiB(DE3)plysS, Rosetta-gami(DE3)placI

 

 

 

 

枯草表達(dá)系統(tǒng)pMA5,pHCMC05pGFP22,pGFP315pHP13,pHP13-43pHY-43pWB980,pAOX1

枯草宿主菌:BS168DB403,BCL,1A75,WB600WB800N

 

昆蟲(chóng)表達(dá)系統(tǒng):pfastBacIpfastIIpfastIIIpfastBacHTApfastBacHTBpfastBacHTCpfastBacdual

 

巨大芽孢桿菌系統(tǒng):pHis1525

巨大宿主菌:YYBM1WH320

 

乳酸球菌表達(dá)系統(tǒng)  pMG36e,NZ9000,MG1363

 

乳酸酵母表達(dá)系統(tǒng)  pKLAC1GG799

 

白色念珠菌系統(tǒng)pCR4,pCR4-GFP,p5921

 

釀酒酵母表面展示系統(tǒng):pYD1,EBY100

 

標(biāo)準(zhǔn)微生物菌株

ATCC27853 銅綠假單胞菌

ATCC29212 糞鏈球菌

ATCC29213 金黃色葡萄球菌

ATCC35218 大腸桿菌

ATCC 35098四聯(lián)球菌

NRRL Y-12968熱帶假絲酵母

NRRL Y-2021德巴利酵母

NRRL Y-1603畢赤氏巴斯德酵母

DSM3586野油菜黃

 

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