一個合格質(zhì)粒的組成要素 復(fù)制起始位點Ori 即控制復(fù)制起始的位點。原核生物DNA分子中只有一個復(fù)制起始點。而真核生物DNA分子有多個復(fù)制起始位點。 抗生素抗性基因 可以便于加以檢測,如Amp+ ,Kan+ 多克隆位點MCS 克隆攜帶外源基因片段 P/E 啟動子/增強(qiáng)子 Terms 終止信號 加poly(A)信號 可以起到穩(wěn)定mRNA作用
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下面所有質(zhì)粒和菌株,均為本公司實驗室親自驗證過,可以提供,載體來源于promega,addgene,clontech,可提供質(zhì)粒圖譜,可提供近萬種質(zhì)粒載體、菌株、基因和細(xì)胞株,并可提供實驗技術(shù)外包服務(wù),如基因克隆、質(zhì)粒構(gòu)建、蛋白原核、真核表達(dá)及純,pQE 載體,原核表達(dá)載體,克隆載體 ,畢赤酵母表達(dá)載體,畢赤酵母宿主菌,釀酒酵母表達(dá)載體 ,釀酒酵母宿主菌,真菌表達(dá)載體,真核哺乳動物熒光載體,真核哺乳動物載體,植物表達(dá)載體,大腸桿菌宿主菌,枯草宿主菌
釀酒酵母表達(dá)載體
pYES2,pYES2/NT,pYES2/CT,pYES3,pYES6, pYCplac22-GFP,酵母載體pAUR123,pRS303TEF,pRS304, pRS305,pRS306,pY13TEF,pY14TEF,pY15TEF,pY16TEF,酵母基因重組表達(dá)載體pUG6, pSH47,酵母單雜載體pHISi,pLacZi,pHIS2, pGAD424, 酵母雙雜交系統(tǒng):釀酒酵母Y187,釀酒酵母AH109;質(zhì)粒pGADT7,pGBKT7;對照質(zhì)粒pGBKT7-53,pGBKT7-lam,pGADT7-T,PCL1,釀酒酵母菌株INVSc1,YM4271, AH109,Y187,Y190, 畢赤酵母表達(dá)載體pPIC9K,pPIC9K-His,pPIC3.5K,pPICZalphaA,B,C,pPICZA,B,C,pGAPZαA,pAO815,pPIC9k-His,pHIL-S1,pPink hc,配套畢赤酵母Pichiapink,畢赤酵母宿主X33,KM71,KM71H,GS115,
原核表達(dá)載體pQE30,31,32,40,60,61,62,等原核表達(dá)載體,包括pET系列,pET-GST,pGEX系列(含GST標(biāo)簽),pMAL系列pMAL-c2x,-c4x,-c4e,-c5x,-p5x,pBAD,pBADHis,pBADmycHis系列,pQE系列,pTrc99a,pTrcHis系列,pBV220,221,222,pTXB系列,pLLP-ompA,pIN-III-ompA(分泌型表達(dá)系列),pQBI63(原核表達(dá)帶熒光)pET3a, pET 3d, pET 11a, pET 12a, pET 14b, pET 15b, pET 16b, pET 17b, pET 19b, pET 20b, pET 21a,b,d, pET 22b, pET 23a, pET 23b, pET 24a,b, pET 25b, pET 26b, pET 27b, pET 28a,b, pET 29a, pET 30a, pET 31b, pET 32a, pET 35b, pET 38b, pET 39b, pET 40b, pET 41a,b pET 42a, pET 43.1a,b pET 44a, pET 49b pET302,303 pET His,pET Dsb,pET GST,pET Trx pQE2, pQE9 pQE30,31,32, pQE 40 pQE70 pQE80L pQETirs system pRSET-A pRSET-B pRSET-C pGEX4T-1,-2,-3,5x-1,6p-1,6p-2,2tk,3c pBV220,221,222 pTrcHisA,B,C pBAD24,34,43 pBAD HisA,B,C pPinPoint-Xa1,Xa2,Xa3 pMALc2x, p2x pBV220 pGEM Ex1, pGEM7ZF(+), pTrc99A, pTwin1, pEZZ18 pkk232-8,pkk 233-3,pACYC184,pBR322,pUC119 pTYB1,pTYB2,pTYB4,pTYB11 pBlueScript SK(+),pBlueScript SK(-) pLLP ompA, pINIIIompA, pMBP-P ,pMBP-C, 大腸桿菌冷激質(zhì)粒: pColdI pColdII pColdIII pColdTF 原核共表達(dá)質(zhì)粒:pACYCduet-1,pETduet-1,pCDFduet-1,pRSFduet-1 Takara公司大腸桿菌分子伴侶: pG-KJE8 pGro7 pKJE7 pGTf2 pTf16 大腸桿菌宿主細(xì)胞: DH JM101 JM103 JM105 JM107 JM109 JM110 *0 *0F BL21(DE3) HB101 ER2529 E2566 C2566 MG1655 XL-10gold XL blue M15 JF1125 K802 SG1117 BL21(AI) BL21(DE3)plysS TG1 TB1 DH(pir) Tuner(DE3) Bl21 codonplusRIPL Novablue(DE3) Rosetta Rosetta(DE3) Rosetta(DE3)plys Rosetta-gami(DE3) Rosetta-gamiB(DE3), Rosetta-gamiB(DE3)plysS Orgami(DE3) OrgamiB(DE3) HMS174(DE3)
植物表達(dá)/RNAi載體農(nóng)桿菌pBI121,pBI121-GFP,pBI101,pBI221,pSN1301, pUN1301,pRTL2 , pRTL2-GFP , pRTL2-CFP, pRTL2-RFP , pRTL2-YFP,pCAMBIA 1300, 1301, 1302,1303,1304,1305, 1381Z,1391Z,2300, 2301,3300,3301,pCAMBIA super1300,pCAMBIA super1300-GFP,pPZP212,pPZP2121,pPZP212-GFP,pGDG,RNAi載體pART27,pHANNIBAL,pKANNIBAL, pFGC5941,pTCK303, pTRV1,pTRV2, T-DNA插入載體(隨機(jī)突變體庫)pSKI015,pSKI074,真菌ATMT載體pBIG2RHPH2-GUS-GFP,pBHt1
枯草芽孢桿菌表達(dá)載體pWB980,pHT43,pHP13,pHP43, pBE2,pMUTIN4,pUB110,pE194,pMA5, pMK3,pMK4,pHT304,pHY300PLK, pBest502,pDG1363,pSG1154,pAX01, pSAS144,pDL,pDG148-stu,pDG641, pAL12,pUCX05-bgaB,pHT01, 配套菌株BS 168,WB600,WB800,WB700, WB800N,1012,FZB42,1A747,廣宿主質(zhì)粒pVLT33
RNAi基因沉默干擾敲除載體pSilencer1.0,pSilencer 2.1-U6 hygro, pSilencer 3.1-H1 hygro,pSilencer 3.1-H1 neo, pSilencer 4.1-CMV neo, pSilencer 4.1-CMV puro pMIR-REPORT Luciferase RNAi載體(oligoengine) pSuper-puro RNAi逆轉(zhuǎn)錄病毒載體(clontech): RNAi-Ready pSIREN-Retro Q, RNAi-Ready pSIREN-RetroQ-ZsGreen(Luciferase shRNA Annealed Oligonucleotide) RNAi慢病毒載體(addgene): pLKO.1
基因cDNA文庫ORF表達(dá)克隆質(zhì)粒,
擁有目前中國zui大的cDNA克隆庫、覆蓋10萬人、鼠全長基因的cDNA克隆和超過25,000個ORF克隆表達(dá)載體。
哺乳動物表達(dá)載體pcDNA3.1+/-,pcDNA4/HisMax B,pSecTag2 A,pVAX1,pBudCE4.1,pTracer CMV2,pcDNA3.1(-)/myc-His A ,pcDNA6-Myc/His B,pCEP4, pIRES,pIRESneo,pIRES hyg3,pCMV-myc,pCMV-HA,pIRES-puro3,pIRES-neo3,pCAGGS哺乳動物雙雜交系統(tǒng)pACT,pBIND,pACT-MyoD,pBIND-Id,pG5luc,pCMV-BD, pCMV-AD, pBD-p53, pFR-luc,Cytotrap Two-Hybrid System:pSos, pSos MAFB, pMyr蛻皮激素誘導(dǎo)系統(tǒng)pIND, pVgRxR,LacSwith II哺乳動物誘導(dǎo)表達(dá)系統(tǒng):pOPRSVI ,pOPI3CAT,pCMVLacI,GeneSwitch System:pSwitch哺乳動物表面展示系統(tǒng):pDisplay, 四環(huán)素調(diào)控系統(tǒng)(Invitrogen):pcDNA4/TO/Myc-His A,pcDNA4/TO/Myc-His B,pcDNA4/TO/Myc-His C,pcDNA4/TO/Myc-His/LacZ,pcDNA6/TR四環(huán)素調(diào)控系統(tǒng)(Clontech):pTet-On,pTet-Off,pTRE2,pRevTRE,pRevTet-On,pRevTet-off信號通路報告載體:pGAS-TA-Luc,pSTAT3-TA-Luc, pISRE-TA-Luc, pTA-Luc,pIκB-EGFP,pNFAT-TA-Luc,pCaspase3-sensor,pAP1(PMA)-Luc;pGL4.26[luc2P/minP/Hygro],pGL4.29[luc2P/CRE/Hygro],pGL4.30[luc2P/NFAT-RE/Hygro],pGL4.75;p53-Luc,pAP-1-Luc, pNF-κB-Luc,pSRE-Luc,pFA2-Elk1,pFC-MEKK,pFR-luc,Gateway系統(tǒng)(invitrogen)pcDNA6.2-GWEmGFP-miR negative, pLenti 6/TR,pcDNA 6.2-GW EmGFP-miR,
乳酸菌表達(dá)載體及各種乳酸菌乳酸桿菌菌株,pNZ8148,pLEISS,pMG36e,pBBR1MCS-5,pBBR1MCS-6,pRV610,pLEM415,pHY300PLK,分泌型乳酸菌表達(dá)載體pVE5523,pPG611.1,pPG612.1等和乳酸桿菌菌株宿主菌NZ9000,MG1363,Lactobacillus casei 1.539,Lactobacillus casei,acidophilus NCFM,1.2,Lactobacillus sakei 23K,L.plantarum,L.rhamnosus GG,B.coagulans,Bifidobacterium bifidum,Bifidobacterium infantis,Lactococcus lactis M17,1663,Lactobacillus reuterii
廣宿主表達(dá)載體鏈球菌表達(dá)敲除載體假單胞菌表達(dá)載體pVLT33,pBBR1MCS-2,3,4,5,6, pJRD215,pJN105,pME6032,Cos載體pLAFR3,pMP2444(GFP), pHY300PLK,pRT102,pRL1063a, 轉(zhuǎn)座子載體pUT-miniTn5,pMGS100, pWHM10,pKC1139,pSET152, pOJ260,pPG611.1,pPG612.1,
腺病毒載體/慢病毒,逆轉(zhuǎn)錄病毒表達(dá)載體及包裝包膜質(zhì)粒,腺病毒系統(tǒng)(Stratagene): pAdEasy-1,pShuttle-CMV,pShuttle,pAdTrack, pAdTrack-CMV, pShuttle-IRES-hrGFP-1、pShuttle-IRES-hrGFP-2、pShuttle-CMV-lacZ, pShuttle-CMV-EGFP-C,pXC1, pBHGE3, 配套大腸桿菌BJ5183,293,293T cell line 腺相關(guān)病毒系統(tǒng)(Stratagene): pAAV-MCS,pAAV-RC,pHelper,pAAV-LacZ,pAAV-IRES-hrGFP,pCMV-MCS, 慢病毒載體:pLVX-DsRed-Monomer-N1,pLVX-IRES-ZsGreen1,pLVX-AcGFP1-N1,Lenti6/v5-EDST-EGFP,pWPXL, FUGW,pLentilox 3.7,RNAi-Ready pSIREN-Retro Q, RNAi-Ready pSIREN-Retro Q-ZsGreen, pSUPER.Retro-GFP/Neo,pSUPER-Retro-Neo, pSUPER.Retro-puro,PLNCX PLNCX2 pMSCV-HYG pMSCV-neo pMSCV-puro pLEGFP-C1 pLOX-CW-CRE pLOX-GFP-IRES-TK pRetroX-IRES-DsRedExpress, pLVX-IRES-mCherry質(zhì)粒載體。 病毒包裝:二質(zhì)粒包裝系統(tǒng)一: psPAX2 pMD2.G 二質(zhì)粒系統(tǒng)二: pCMV-dR8.2 dvpr pCMV-VSVG 三質(zhì)粒包裝系統(tǒng)具有更高的包裝效率: pMDLg/pRRE pRSV-Rev pMD2.G Invitrogen包裝系統(tǒng): PLP1 PLP2 PLP/VSVG
基因重組載體敲除載體自殺質(zhì)粒RED重組系統(tǒng)載體pKD3,pKD13,pKD4,pCP20,pKD46, pCVD442,pk18mobsacB,p2NIL, pMAD(革蘭氏陽性菌基因敲除),pBT2,RN4220,pKC1139,pOJ260, pJQ200SK,pSET152,pWHM10, pk18mobsacB, pGOAL17,pGOAL19,基因打靶載體PL253,PL451,PL452,PL453,配套菌株EL250,EL350,pMLM290,292, KJBAC1,pST1374
熒光蛋白表達(dá)載體熒光定位質(zhì)粒報告基因載體pEGFP,pEYFP,pECFP,pDsRED, pmCherry,pAcGFP,hrGFP,ZsGreen,pDsRed1-C1, pDsRed1-N1, pDsRED-Monomer-N1, pEYFP-C1, pEYFP-N1, pEGFP-1,pEGFP-C1, pEGFP-N1, pEGFP-N3, pcDNA-EGFP1,pECFP-N1,pECFP-C1, pAcGFP1-1 pDsRED2-ER, pDsRED2-Mito, pDsRED2-Nuc,pDsRED2-Peroxi , pEYFP-Golgi, pEYFP-Mem, pEYFP-ER , pEGFP-Actin, pECFP-ER, pIRES2-EGFP, pLEGFP-C1,pLEGFP-N1,pDsRED-Express-N1,pIRES2-DsRed,pIRES-AcGFP1,pmCherry-C1,N1 啟動子增強(qiáng)子報告載體(Promega,帶有Luc熒光報告基因): pGL3-basic,pGL3-control,pGL3-enhancer,pGL3-promoter,pRL-TK,pRL-CMV,pRL-SV40, 信號通路報告載體(Clontech): pGAS-TA-Luc,pSTAT3-TA-Luc, pISRE-TA-Luc, pTA-Luc,pIκB-EGFP, pNFAT-TA-Luc,pCaspase3-sensor,pAP1(PMA)-Luc 信號通路報告載體(Promega):pGL4.26[luc2P/minP/Hygro],pGL4.29[luc2P/CRE/Hygro],pGL4.30[luc2P/NFAT-RE/Hygro],pGL4.75 信號通路報告載體(Stratagene): p53-Luc,pAP-1-Luc,pNF-κB-Luc,pSRE-Luc, pFA2-Elk1,pFC-MEKK,pFR-luc
昆蟲表達(dá)載體桿狀病毒表達(dá)載體系統(tǒng)pFastBac1,pFastBac1-Gus,pFastBacHT A pFastBacHT-CAT,pFastBacDual,pBlueBacHis2 A DH10Bac配套大腸桿菌,pMIB v5-His B ,pIZT/V5-His,
Taq,Pfu高表達(dá)載體菌株KOD, T4連接酶高表達(dá)質(zhì)粒載體,高產(chǎn)基因工程菌株、酶基因KOD,T4 kinase激酶,Tth反轉(zhuǎn)錄酶MMLV,Klentaq PCR酶,Cre酶,RNA inhibitor抑制酶,DL2000質(zhì)粒
BiFC雙分子熒光表達(dá)載體FRET系統(tǒng)載體pBiFC-VC155,pBiFC-VN173,pBiFC-bFosVC155,pBiFC-bFos(deltaZIP)VC155,pBiFC-bjun-VN173,pCE-BiFC-VN173,pCE-BiFC-VC155,
真菌表達(dá)載體黑曲霉米曲霉黃曲霉表達(dá)質(zhì)粒pBC-hygro,pAN7-1,pBARGPE1, 真菌ATMT農(nóng)桿菌介導(dǎo)轉(zhuǎn)化載體 pBHt1,pBIG2RHPH2-GFP-GUS等,
酵母單雜交三雜交載體,酵母雙雜交系統(tǒng)pGBKT7,pGADT7,pCL1,pGBKT7-53,pGADT7-T,pGBKT7-Lam,配套釀酒酵母AH109 酵母單雜交系統(tǒng)(Clontech):pHISi,pLacZi,pHis2,pHIS2.1 酵母三雜交系統(tǒng):pBridge, YBZ1菌株 Cytotrap Two-Hybrid System(Stratagene): pSos, pSos MAFB, pMyr
噬菌體展示系統(tǒng)載體1.噬菌體展示抗體scFv 質(zhì)粒:pCANTAB5E 宿主菌:TG1 輔助噬菌體:M13K07 可溶表達(dá)菌:HB2151 2.噬菌體展示抗體Fab 質(zhì)粒:pcomb3 宿主菌:XL1-Blue 輔助噬菌體:VCSM13
pEVOL,pBudCE4.1,pBudCE4.1-EGFP,Zeocin,pEGFP-C3,2,1,pmCherry-C1,-N1,pLOVE-miR302/367,pDsRed2-N1,pAcGFP1.1,TALE toolbox kit,pIRES-hyg3,pIRES-Neo3,pIRES-puro3,pSIREN-RetroQ-ZsGreen,GST 14-3-3,pDsRed-Monomer-N1,M50 super8x TOPFlash,pRK793,pCMV-tag5B,pCMV-tag2B,pCMV-N-HA,pUAST,pw-25,PAC-5.1-HISA,pSLIK-Neo,pCMV-myc-cyto,pCMV-myc-nuc,疫苗表達(dá)載體pVAX1,pAdEasy-1,pShuttle,pAdTrack,pAdTrack-CMV,-polyA,pShuttle-EGFP,BJ5183,pXC1,pBHGE3,pSUPER-retro-GFP-Neo,pORF-lacZ,pORF-TK,pCOX2-Luc,pcDNA3.1(-)-myc-His A,pcDNA3.1-p300,pINP, pOprE, pCR2.1 TOPO,pLP1,pLP2,pLP/VSVG,Stbl3,plenti6/V5-DEST,pLVX-shRNA1,2,pLVX-IRES,tdTomato,FUW-teto-hOCT4,hSOX2,hMYC,hKLF4,FUW-M2rtTA,pSecTag2 A,pEYFP-C1,pEGFP-N1,-C1,pcDNA3.1-V5-His A,B,C,pcDNA3.1-EGFP,pLEGFP-C1,α-MHC Promoter,Marc-145猴腎細(xì)胞,Sf9,BmN昆蟲家蠶細(xì)胞,酵母載體pYES2,pYES2.0,INVSc1,pYES3,pYES6,pRS41H,pRS303,304,305,306,pRS424,425,426,414,415,416,pY13,14,15,16,YBZ1,L40酵母菌,pESC-His,pYIP5,pHIS2,pHIS2.1,pHISi,pLacZi,酵母重組載體pUG6,pSH47,植物載體pCAMBIA1300-EGFP, pTCK303,pTRV1,pTRV2,pFGC5941,pUN1301,pSN1301,C58C1,R1601,AGL1農(nóng)桿菌感受態(tài),原核載體pQE3,pQE30,31,32,pQE60,61,62,M15,DH10Bac,Origami(B)DE3,XL1-Blue,Rosetta gamiB(DE3)pLysS,TKB1,ER2566,DB3.1,JM105,*0,DH,DHlpha lambda pir,C43(DE3)菌株感受態(tài),pTrcHis A,B,C,pTWIN1,pTYB1,pTYB11,pACYC184,pMAL-c5x,c2x,p5x,c4x,pBV220,221,222,pCold I,II,III,TF,pET22a,pET29a,pET28a,pET39b,pET41a,pET42a,p1,pET-sumo,pBADHis A,B,C,pBAD24,34,pETDuet-1,pRSFDuet-1,pCDFDuet-1,pACYCDuet-1,原核熒光pQBI63,pMP2444,鏈球菌表達(dá)、敲除載體pKC1139、pOJ260、pWHM3,pSET152,pVA981,多宿主載體pGOAL19,pBBR1MCS-2,-5,-6,pDSK519,pLA2917,pRK2013,pVLT33,pMAD,RN4220金葡菌株,真菌載體pBARGPE1,pAN7-1,pBHt1,pBIG2RHPH2-GFP-GUS,乳酸菌載體MG1363,pNZ8148,NZ9000,pNZ9530,pMG36e,枯草芽孢桿菌載體pHT43,pWB980,pHT300PLK,pMA5,pUTminiTn5,巨大芽孢桿菌表達(dá)載體pHIS1525,WH320菌,谷氨酸棒狀桿菌表達(dá)載體pEC-XK99E,敲除載體pk18mobsacB,