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世聯博研（北京）科技有限公司 |
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—— 銷售熱線 ——
18618101725 |
PETAKA G3低氧高氧細胞培養板，缺氧缺氧培養板，細胞培養技術革新 --無需提供CO2的高仿真體外培養系統！獲得美國、歐洲廣大生物科學家的青睞！
貨號 | 描述 | 規格 | 特點 |
PET025 | Celartia Petaka G3 LOT 細胞培養板 -* | 25片/盒 | 無需CO2培養箱提供額外的CO2； 無需濕度培養箱提供額外的濕度； 無需凍存或干冰運輸，可直接進行活細胞株運輸； 優化的培養條件，高仿真的體內模擬； 可雙面培養； 體外細胞休眠效果好； 可提前把培養基注入板中保存； 操作簡單、安全和功能多樣； 條形碼跟蹤； |
PET300 | 25片/盒×12盒 | ||
PET120 | 25片/盒×48盒 | ||
PET025H | Celartia Petaka G3 HOT 細胞培養板 -*
| 25片/盒 | 功能等同于LOT系列； 特別適用于高需O2的細胞培養，例如：攜帶桿狀病毒載體的細胞/HEPG2細胞/ 攜帶病毒的細胞培養 適用于病毒培養； |
PET300H | 25片/盒×12盒 | ||
PET120H | 25片/盒×48盒 | ||
PET025F | Celartia Petaka G3FLAT細胞培養板 -* | 25片/盒 | 功能等同于HOT系列； 培養板內部無處理不利于細胞貼壁培養； 使用于血細胞；癌細胞；雜交瘤細胞等懸浮細胞的培養，對于藥物應用方面，適用于分泌特定產品的懸浮細胞培養； |
PET300F | 25片/盒×12盒 | ||
PET120F | 25片/盒×48盒 |
培養系統 | T-培養瓶75 | Petaka培養系統 |
加樣口 | 廣口，螺帽 | 密閉，針頭/槍頭注射 |
排氣系統 | 無特定排氣口 | 排氣裝置 |
尺寸 | 139×86×22（mm） | 128×86×5.2（mm） |
有效面積 | 75cm2 | 150cm2 |
zui大細胞數 | 1.5×107 | 2.5×107 |
培養基體積 | >30ml | 20ml |
培養方式 | 水平培養 | 可直立/水平培養 |
密閉性 | 差 | 優 |
是否可離心 | 否 | 是 |
功能 | 培養 | 培養+其他（凍存/運輸/分選/觀察……） |
其他 | 單邊貼壁培養 | 雙邊貼壁培養 |
Petaka培養系統不需要CO2培養箱或者濕度培養箱，僅在合適的培養溫度下即可生長，使得培養環境簡易化；根據細胞的特性，可靈活選擇合適的含氧條件（低氧/高氧），模擬體內培養環境；密閉式加樣口和排氣口，保證了培養環境的封閉性，減少污染幾率。
Petaka 細胞培養系統中Micro-tubular gas diffusion channel，是Celartia公司的技術，自動平衡培養基質中的溶解氧含量——45mmHg，嚴格模擬細胞的天然生長條件。同時Petaka 細胞培養系統可根據培養細胞的類型，耗氧情況，從而減少培養系統中的氧氣含量，比如常規的21%的氧氣含量降低至5%或2%。
觀點一： 京都大學教授山中伸彌等人在iPS細胞研究過程中，發現機體內的干細胞總是集中于氧氣相對少的地方。于是，他們在利用人體皮膚細胞培養iPS細胞時把培養環境的氧濃度從通常的21％降到5％，發現iPS細胞的生成效率可提高到原來的2．5倍至4．2倍。
觀點二： 低氧是生物體常見的一種生理和病理現象，不同組織的細胞生長在不同的氧濃度下，在探討氧環境是否影響相關干細胞分化上值得我們干細胞工作者們做深入研究，尤其是在胚胎發育過程中氧濃度的變化對相關組織的形成與發育研究在不久的將來定會有重大的突破。
觀點三： 低氧環境對胚胎早期發育過程影響很大，像體外培養的細胞（常壓/常氧），與體內相比，表觀修飾發生了巨大變化。
The cell culture device PetakaG3 is an innovative bioreactor designed for mammalian cell culture 
in physiologic tissue conditions. The distinctive feature of PetakaG3 is that being a disposable device incorporates an automatic gas diffusion control system (AGD-CS) that works interactively with the cells growing and living inside, WITHOUT HYPOXIA CAMBERS, GLOVE BOXES OR THREE-GAS INCUBATORS
For cells cultured in PetakaG3 and incubated in regular 21% oxygen atmosphere the AGD-CS provides:
1) Media dissolved oxygen concentration within the limits of the physiologic oxygen tensions in physiologic tissues (from 75 mmHg down to 15 mmHg) monitorable with a dissolved oxygen fluorescent sensor dot. Because this advanced trait PetakaG3 is the safest, smallest, unique portable disposable hypoxia chamber, also available with integrated DO sensor.
Average DO = 1 mg/L (0.5 to 1.1 mg/L depending on cell type), equivalent to average 22.7 mmHg (Torr) in tissues or equivalent to average 3.11 %O2 in hypoxia chambers.
PDF. Additional information
2) Media dissolved CO2 concentration, enough to maintain a physiologic pH without external extra-supply of carbon dioxide gas.
3) Controlled media evaporation even at 2% to 10% atmospheric Relative Humidity (RH).
PetakaG3 allows cultures of two different cell types in the same device. Both cell layers are separated by 3mm of media, which is appropriate to study intercellular interactions through diffusible factors and exosome exchange. PetakaG3 is ideal for transfection of both cell layers with two different gene constructs in the same device with negligible cross transfection
Global specifications of PetakaG3 LOT:
Humidity autocontrolled, no added humidity required (not influenced by environment)
The cell culture device Petaka G3 with orange color port.
PetakaG3™ HOT bioreactor is manufactured from a special co-polymer which has a particularly high diffusion rate of oxygen and carbon dioxide through the walls of the device.
Average DO = 3 mg/L (2.8 to 3.2 mg/L depending on cell type), equivalent to 66.5 mmHg in tissues or equivalent to 9.33 %O2 in hypoxia chambers.
Because of its high diffusion coefficients requires to be incubated in 5% CO2 with saturated relative humidity to avoid dehydration.
PetakaG3™ HOT has a gas self regulating system that maintains the level of dissolved oxygen in the cell culture media like in all Petaka G3, but the minimal level that can reach is higher, for a given cell type and cell density. Example: in the same cell culture conditions (cell type, cell density and media type) when Petaka G3 balances the oxygen exchange at 1ppm, Petaka G3 HOT will hold it at 3ppm, maintaining the median level of dissolved oxygen in the highest limit of the physiologic oxygen tension of the tissues, equivalent to 10% concentration of oxygen in the open atmosphere.
PetakaG3™ HOT bioreactor is particularly suited for culturing cells with a high aerobic metabolism, for example primary liver cell cultures, hematopoietic bone marrow cells, well differentiate keratinocytes, hybridomas, Pancreatic islets, etc.
Cells cultured for a long time in traditional flasks and dishes, exposed to 21% oxygen of open atmosphere, are metabolically adapted to such environment, far from the in vivo reality and behavior.
Special applcations Cell culture devices.-
The PetakaG3™ FLAT bioreactor (For Low ATachmet) has all of the same size and structure characteristics as the PetakaG3™ either LOT or HOT bioreactor, except that the interior surfaces are not treated to promote cell adherence to its walls.This special bioreactor is produced with a flat polymer with a very low surface tension.
Applications:
Suspended cell cultures such as; Blood cell preparation, Leukemia Cells, Hybridomas Cells, Lymphocyte culture and activation for kariotyping.
Special cells necessary for the bio-synthesis of specific products for pharmaceutical applications.
Cultures of cells with self agregation such as spheroids, 3D cultures, pseudo-tissues, etc.
The cell culture device PetakaG3™ ET™ bioreactor is a PetakaG3™ that includes inside the cell culture chamber a set of free steel doped micro-bars (stirrer bars) that can be moved by magnetic fields.
The Petaka™ ET bioreactor is used in conjunction with either an automatic magnetic random scanner (Cell Flipper) that drags the stir bars across the internal surface of Petaka or with a manual magnetic tablet (Cell lifter) on which the user drags the Petaka to detach the cells.
The turbulences created by the stir bar motion over the cells are strong enough to induce cell enzyme free cell detachment without the need for chemicals. Depending on cell type the automatic scanning is fast for cell harvesting and preserves a high yield of unharmed detached cells that can be directly transferred to new bioreactors without washing.
The manual Cell Lifter is used for detaching fragile cells carefully; the operator controls the speed and the specific areas of the cell monolayer to be detached.
Petaka G3 ET is ideal for enzyme free detachment for any kind of cell line, and extremely well suited for Stem cell cultures, also allows for safe and frequent cell culture samplings during growth, and in vitro evaluation of wound healing.
The cell culture device Petaka G3 MATRIX could be LOT or HOT
The PetakaG3™ MATRIX bioreactor has all of the same size and structure characteristics as the PetakaG3™ bioreactor.
PetakaG3™ MATRIX is Coated with Specific Molecular Matrix. These are several types of Petaka internally coated with specific molecular matrix incluiding collagen, fibronectin, poly-L-lysine, matrigel, gelatin, laminin.
This product is usually best suitable for growing different types of stem cells.
Because of the specialty matrices involved, these bioreactors are only available on a made-to-order basis.
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