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恒遠產(chǎn)品文獻:昆蟲P450s,CarE,GST,ELISA試劑盒引用文獻
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【文獻標題】Effects of sublethal concentrations of cyantraniliprole on the biology and metabolic enzyme activities of Laodelphax striatellus (Fall′en)
【作者】Xueting Wang , Xiang Liu , Chang Liu , et.al
【作者單位】山東農(nóng)業(yè)大學(Shandong Agricultural University)
【文獻中引用產(chǎn)品】
昆蟲P450側(cè)鏈裂解酶(P450s)ELISA試劑盒 酶活
昆蟲羧酸酯酶(CarE)ELISA試劑盒 酶活
昆蟲S轉(zhuǎn)移酶(GST)ELISA試劑盒 酶活
【關(guān)鍵詞】Cyantraniliprole ,Laodelphax striatellus ,Life table ,Sublethal effect
【DOI】doi.org/10.1016/j.cropro.2022.105964
【影響因子(IF)】3.03
【出版期刊】《Crop Protection》
【產(chǎn)品原文引用】
The activities of P450s, CarE, and GST were examined using an ELISA kit (Shanghai Hengyuan Biotechnology Co., Ltd., Shanghai, China) following the manufacturer’s instructions. The diluted standard substance and reagents in the kit and enzyme protein were added to a 96-well plate according to the manufacturer’s instructions. After the reaction had finished, the OD value at 450 nm was determined using a microplate reader. A standard curve was drawn according to the concentrations of the standard substance and the corresponding OD values, and then the enzyme activities were calculated based on the standard curve.
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