| 供貨周期 |
一周 |
規(guī)格 |
T25 |
| 貨號 |
SH-2 |
應用領域 |
醫(yī)療衛(wèi)生,化工,生物產業(yè),制藥 |
| 主要用途 |
科學研究 |
HB-10743 SH-2 鼠雜交瘤細胞，ATCC 細胞|細胞系|細胞株|腫瘤細胞株|細胞|貼壁細胞|懸浮細胞|；細胞庫管理規(guī)范，提供的細胞株背景清楚，提供參考文獻和培養(yǎng)條件
HB-10743 SH-2 鼠雜交瘤細胞的詳細介紹
HB-10743 SH-2 鼠雜交瘤細胞
| ATCC® Number: | HB-10743™ | Price: | $399.00 |
| Designations: | SH2 |
|
| | Depositors: | Osiris Therapeutics, Inc. |
|
| | Isotype: | IgG1 |
|
| | Biosafety Level: | 1 |
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| | Shipped: | frozen |
|
| | Medium & Serum: | See Propagation |
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| | Growth Properties: | suspension |
|
| | Organism: | Mus musculus （B cell）; Mus musculus （myeloma） （mouse （B cell）; mouse （myeloma）） |
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| | Morphology: | lymphoblast
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| | Source: | Cell Type: hybridoma: B lymphocyte; |
|
| | Cellular Products: | immunoglobulin; monoclonal antibody; against human mesenchymal stem cells |
|
| | Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | | HB-10743 |
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| | Tumorigenic: | Yes |
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| | Comments: | Animals were immunized with human mesenchymal stem cells. Spleen cells were fused with Sp2/0-Ag14 mouse myeloma cells. The antibody with mesenchymal stem cells from bone marrow. |
|
| | Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:fetal bovine serum to a final concentration of 20% 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere ATCC tested fetal bovine serum is available as ATCC Catalog No. 30-2020.
Temperature: 37.0°C
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|
| | Subculturing: | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 2 X 10 exp5 and 1 X 10 exp6 cells/ml. |
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| | Preservation: | culture medium 95%; DMSO, 5% |
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| | Related Products: | recommended serum:ATCC 30-2020 Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 46-X |
|
| | References: | 22086: Caplan AI, Haynesworth SE. Human mesenchymal stem cells. US Patent 5,486,359 dated Jan 23 1996 49752: Haynesworth SE, et al. Cell surface antigens on human marrow-derived mesenchymal cells are detected by monoclonal antibodies. Bone 13: 69-80, 1992. PubMed: 1316137 |
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