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ATCC 750 熱帶念珠菌使用說(shuō)明
組成：凍干菌種瓶、菌種復(fù)溶液（用于復(fù)溶菌種及保存用）、使用說(shuō)明書(shū)。
方法：建議用下列方法恢復(fù)培養(yǎng)冷凍干燥的菌種。
復(fù)蘇菌種前應(yīng)準(zhǔn)備適于該菌種生長(zhǎng)的固體或液體培養(yǎng)基和相應(yīng)的培養(yǎng)設(shè)備，所有操作均應(yīng)在符合生物安全保護(hù)及無(wú)菌條件下進(jìn)行。
－、疫苗瓶開(kāi)封
1．拉開(kāi)易撕鋁蓋，用浸過(guò)75％酒精的脫脂棉擦凈丁基膠塞及疫苗瓶。
二、菌株恢復(fù)培養(yǎng)
1．吸取適量的菌種復(fù)溶液，滴人疫苗瓶?jī)?nèi)，輕輕振蕩，使凍于菌體溶解呈懸浮狀。
2．取適量菌體懸浮液(建議岢養(yǎng)菌種可適當(dāng)加大用量)，移植于沙保羅瓊脂平板上，放置在30-35℃培養(yǎng)24-48小時(shí).真菌在23-28℃培養(yǎng)箱中培養(yǎng)24-72小時(shí)（必要時(shí)可延長(zhǎng)培養(yǎng)時(shí)間）
3．斜面（一代）放于4℃，并用封口膜密封平板，每半月轉(zhuǎn)接一次，代數(shù)一次類(lèi)推，建議使用菌種代數(shù)不超過(guò)5代，5代后菌種應(yīng)銷(xiāo)毀。
三、注意事項(xiàng)
1． 菌種活化前，請(qǐng)保存在2-8℃的環(huán)境下。
2． 嗜血桿菌、奈瑟氏菌屬等初代培養(yǎng)需要5-10%CO2環(huán)境和的培養(yǎng)基，建議使用培養(yǎng)基。
3． 厭氧菌的培養(yǎng)，如無(wú)特別說(shuō)明，自開(kāi)封至接種完成，均需以無(wú)氧氣體充填，以保持厭氧狀態(tài)。
4． 某些菌種經(jīng)過(guò)冷凍干燥保存后，延遲期較長(zhǎng)，需連續(xù)兩次繼代培養(yǎng)才能正常生長(zhǎng)后使用。
ATCC 750菌種。熱帶念珠菌
Candida tropicalis (Caslani) Berkhout, anamorph (ATCC® 750™)
| Deposited As | Monilia tropicalis (Caslani) Caslani et Chalmers, anamorph |
|---|---|
| Strain Designations | [1909, ATCC 4563, ATCC 7349, CBS 94, CCRC 20520, DSY 59, IFO 1070, IFO 1400, JCM 1541, NRRL Y-12699, NRRL Y-12968, NRRL Y-607] |
| Application | Assay of chloro-8-quinolinol Bacterial resistance testing Degrades xylose D-xylose Fungus resistance testing adhesives Produces aspartic proteinases aspartyl proteinases Produces ethyl alcohol ethanol Produces xylitol Susceptibility disc testing Susceptibility testing Transformation host Produces xylitol from D-xylose Control strain for typing systems Produces ethanol from D-xylose Control strain to test oxygen availability in bioreactor in space laboratory Reference strain for Clinical and Laboratory Standards Institute(CLSI)-developed Antifungal Susceptibility Testing. |
| Biosafety Level | 1 ATCC 750菌種。熱帶念珠菌 |
| Product Format | freeze-dried |
| Storage Conditions | Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C |
| Type Strain | yes (type strain) |
| Genotype | ade2/ade2 acp/acp delta::lambda/delta::lambda |
|---|---|
| Preceptrol® | yes |
| Genome Sequenced Strain | Yes |
| Comments | Fluconazole-sensitive Utilizes phenol, catechol and 3- and 4-methylcatechols as sole carbon source Esterase activity MIC of 2-hydroxychalcone, 75 mcg/ml Adherence to lysophospholipids Genome sequencing strain (Genolevures Consortium, France; Wellcome Trust Sanger Institute, UK). For additional infromation, see CLSI M27-A2 (Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts; Approved Standards-Second Edition). Mitochondrial genome sequenced strain |
| Medium | ATCC® Medium 200: YM agar or YM broth ATCC ® Medium 1245: YEPDATCC ® Medium 28: Emmons' modification of Sabouraud's agar |
|---|---|
| Growth Conditions | Temperature: 25°C Atmosphere: Typical aerobic |
