用途:用于快速檢測牛奶、奶粉等樣品中的三聚氰胺殘留。
原理: 本試劑盒采用間接競爭ELISA 方法,在微孔條上預包被偶聯抗原,樣本中的殘留物三聚氰胺將和微孔條上預包被的偶聯抗原競爭抗三聚氰胺抗體,加入酶標記物后,用TMB 底物顯色,樣本吸光值與其所含殘留物三聚氰胺的含量成負相關,與標準曲線比較即可得出相應殘留物三聚氰胺的含量。
試劑盒特性:
u 試劑盒靈敏度:0.5ppb
u 孵育溫度: 25℃
u 孵育時間: 30min~15min
u 樣本檢測下限
牛奶··············································· 1 ppb
奶粉·············································· 10ppb
u 交叉反應率
三聚氰胺········································· 100%
u 樣本回收率
牛奶······································· 90% ±25%
奶粉······································· 80% ±25%
試劑盒組成:
1 | 微量測試孔 | 每條8孔,一板12條 | |
2 | 標準液×6瓶 (1ml/瓶) | 0ppb | 0.5ppb |
1.5ppb | 4.5ppb | ||
13.5ppb | 40.5ppb | ||
3 | 酶標記物 | 7ml | 紅色帽 |
4 | 抗體工作液 | 7ml | 藍色帽 |
5 | 底物A液 | 7ml | 白色帽 |
6 | 底物B液 | 7ml | 黑色帽 |
7 | 終止液 | 7ml | 黃色帽 |
8 | 20X濃縮洗滌液 | 40ml | 白色帽 |
樣本前處理及酶標免疫分析程序:
詳見產品說明書。
儲藏條件和保質期:
1、儲藏條件:試劑盒于2~8℃保存,不要冷凍。
2、保質期:該產品有效期為1 年,生產日期見包裝盒。