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| Catalogue Number | CYT-261 |
| Synonyms | BMP-2, BMP2A. |
| Introduction | BMP2 belongs to the transforming growth factor-beta （TGFB） superfamily. Bone morphogenic protein induces bone formation. BMP2 is a candidate gene for the autosomal dominant disease of fibrodysplasia （myositis） ossificans progressiva. |
| Description | Bone Morphogenetic Protein-2 Human Recombinant produced in E.Coli is a homodimeric, non-glycosylated, Polypeptide chain containing 115 amino acids and having a molecular mass of 26018 Dalton. The BMP-2 is purified by proprietary chromatographic techniques. |
| Source | Escherichia Coli. |
| Physical Appearance | Sterile Filtered White lyophilized （freeze-dried） powder. |
| Formulation | BMP-2 was lyophilized from a concentrated （1mg/ml） sterile solution containing 20mM NaAc pH=4. |
| Solubility | It is recommended to reconstitute the lyophilized Bone Morphogenetic Protein-2 in sterile 20mM AcOH （acetic Acid） not less than 100μg/ml, which can then be further diluted to other aqueous solutions. |
| Stability | Lyophilized Bone Morphogenetic Protein-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BMP2 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles. |
| Purity | Greater than 95.0% as determined by（a） Analysis by RP-HPLC. （b） Analysis by SDS-PAGE. |
| Amino acid sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Met-Gln-Ala-Lys-His. |
| Biological Activity | 1. The ED50 as determined by the cytolysis of MC3T3-E1 cells is < 50 ng/ml. 2. When implanted in the mouse thighbone muscle and grow for 14 days, Muscle tissue was harvested and examined biochemically for calcium uptake. Our unit defined as 1 microgram calcium absorbed. The specific activity is more than 1000 Units/mg. 3. The activity of BMP-2 was tested using alkaline phosphatase, the activity in mouse muscle myoblast C2C12 cell line was found to be > 1 x 100,000 U/mg protein. 4. The ectopic osteogenesis capacity is 1000mg fresh bone per 1mg of BMP-2. |
| Protein content | Protein quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 1.4 as the extinction coefficient for a 0.1% （1mg/ml） solution. This value is calculated by the PC GENE computer analysis program of protein sequences （InliGenetics）. 2. Analysis by RP-HPLC, using a standard solution of BMP-2 as a Reference Standard. |
| Usage | ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
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