人乳腺珠蛋白A(SCGB2A2)ELISA kit使用操作

• 產品名稱:

  人乳腺珠蛋白A(SCGB2A2)ELISA kit

• 英文名稱:

  Human Mammaglobin-A(SCGB2A2) ELISA kit

• 貨號:

  CSB-EL020817HU

• 別名:

  MGB1, MGC71974, UGB2, mammaglobin 1|mammaglobin A

• 規格:

  96T

• 中文價格:

  3600

• 種屬:

  Human

• 待測物名稱:

  secretoglobin, family 2A, member 2

• 縮寫:

  SCGB2A2

• 檢測范圍:

  0.312 ng/ml-20 ng/ml

• 靈敏度:

  0.078ng/ml

• 反應時間:

  1-5h

• 所需樣本體積:

  50-100ul

• 檢測波長:

  450 nm

• 標準曲線:

  These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

  ng/ml OD1 OD2 Average Corrected   20 2.704 2.617 2.661 2.534   10 2.119 2.242 2.181 2.054   5 1.806 1.704 1.755 1.628   2.5 1.373 1.369 1.371 1.244   1.25 0.928 0.864 0.896 0.769   0.625 0.545 0.519 0.532 0.405   0.312 0.287 0.257 0.272 0.145   0 0.121 0.132 0.127

• 精密度:

  Intra-assay Precision (Precision within an assay): CV%<8%
  Three samples of known concentration were tested twenty times on one plate to assess.
  Inter-assay Precision (Precision between assays): CV%<10%
  Three samples of known concentration were tested in twenty assays to assess.

• 線性度:

  To assess the linearity of the assay, samples were spiked with high concentrations of human SCGB2A2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  		Sample		Serum(n=4)
  1:1		Average %		88
  		Range %		84-92
  1:2		Average %		90
  		Range %		86-94
  1:4		Average %		95
  		Range %		91-99
  1:8		Average %		97
  		Range %		93-102

• 回收率:

  The recovery of human SCGB2A2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
  Sample Type		Average % Recovery		Range
  Serum (n=5)		97		93-102
  EDTA plasma (n=4)		90		85-95

• 研究領域:

  Others