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小鼠成纖維細胞;NCTC clone 929 [L cell, L-929, derivative of Strain L]
成纖維細胞
貼壁生長
1948年三月建立了NCTC clone 929(小鼠結締組織),小鼠成纖維細胞;NCTC clone 929 [L cell, L-929, derivative of Strain L]細胞系L的克隆。細胞系L是zui早建立的連續培養細胞系之一,而clone 929是zui早的克隆株。 從一只100日齡的雄性C3H/An小鼠的正常皮下疏松結締組織入脂肪組織中建立了親本細胞系L。 第95代的細胞系L使用毛細管法分離單細胞建立了clone929。 檢測發現鼠痘病毒陰性。
MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle's Balanced Salts) 馬血清,10%
消化3-5分鐘。1:2。3天內可長滿。
560
*培養基+8%DMSO
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