產地：Roche
貨號：11684809910
Product Description
Sample material: Cytospin and cell smear preparations, adherent cells grown on slides, and frozen and paraffin-embedded tissue sections.
Contents
Enzyme Solution （TdT）, 5 vials
Label Solution （fluorescein-dUTP）, 5 vials
Converter AP （anti-fluorescein antibody-AP）, ready-to-use
Principle
The In Situ Cell Death Detection Kit, AP is based on the detection of single- and double-stranded DNA breaks that occur at the early stages of apoptosis.
Apoptotic cells are fixed and permeabilized. Subsequently, the cells are incubated with the TUNEL reaction mixture that contains TdT and fluorescein-dUTP. During this incubation period, TdT catalyzes the addition of fluorescein-dUTP at free 3'-OH groups in single- and double-stranded DNA. After washing, the label incorporated at the damaged sites of the DNA is marked by an anti-fluorescein antibody conjugated with the reporter enzyme alkaline phosphatase. After washing to remove unbound enzyme conjugate, the AP  retained in the immune complex is visualized by a substrate reaction.
Application
Qualitative detection of apoptosis at the single-cell level by light microscopy.