產地：Roche
貨號：11758888001
Contents
HNPP, 0.5 ml HNPP （10 mg/ml; 23.8 mM） in dimethylformamide （DMF）
Fast Red TR, 100 mg Fast Red TR, powder
Quality
Purity:
HNPP: >98% （HPLC）
Fast Red TR: >90%
Application
In combination with Fast Red, HNPP is used for fluorescent detection of a nonradioactively labeled nucleic acid probe in in situ hybridization. HNPP/Fast Red gives a bright, long-lasting red or orange signal withstanding long exposures to UV light. HNPP alone can also be used for membrane blotting applications in which it gives a precipitable distinct signal.  It is especially suited for highly sensitive detection of nonradioactively labeled nucleic acids in
Fluorescence in situ hybridizations （FISH）
Southern blot
Northern blot
Colony or plaque hybridizations
Nonradioactive DNA sequencing blots.
The sensitivity of detection of nonradioactive Southern blots is equivalent to NBT/BCIP and CSPD, after a 2-hour to overnight incubation.
Product Description
Evaluation: fluorimetric; absorbance max. 290 nm, emission max. 509 nm
Formula: HNPP: C23H18NO5P, Fast Red TR: C7H6Cl2N2 x 1/2 ZnCl2
Molecular weight: HNPP: Mr = 419.37, Fast Red TR: Mr = 257.2
Reaction product:
Color: bright, long-lasting red or orange signal