產品名稱：Cyclic GMP EIA Kit
產品貨號：581021
Cayman產地：美國
Cyclic GMP EIA Kit
Cayman Chemical Item Number 581021
Description
Limit of detection: 80% B/B0: 1 pmol/ml · Sensitivity: 50% B/B0: 4.6 pmol/ml · The Cayman Chemical cGMP Assay is a competitive EIA that can be used for quantification of cGMP directly obtained from cell lysates, tissue homogenates, plasma, or urine. The EIA typically displays an IC50 （50% B/B0） of approximay 6 pmol/ml and a detection limit （80% B/B0） of less than 2 pmol/ml. Since the antibody used in this assay was prepared against a cGMP-carrier protein conjugate, antibody binding is increased if an acetyl group is present on the 2' hydroxyl group of the cGMP. The optional acetylation procedure for both samples and standards increases the sensitivity of the assay approximay 10 fold. A protocol for acetylating both the standards and samples prior to performing the assay is provided. Basal levels of cGMP in cell lysates can often be measured without acetylation, but results will depend on the type and number of cells being utilized. Plaets produce approximay 1.5-2.5 pmol cGMP/109 plaets under basal conditions.1,2,3 Cells such as NG108-15 cells and monocytes produce considerably more cGMP than plaets （approximay 0.1-1 pmol/106 cells）.4,5 1 Chen, L., Salafranca, M.N., and Mehta, J.L. Cyclooxygenase inhibition decreases nitric oxide synthase activity in human plaets. Am J Physiol 42 H1854-H1859 （1997）.
2 Miller, O.V., and Gorman, R.R. Modulation of plaet cyclic nucleotide content by PGE1 and the prostaglandin endoperoxide PGG2. J Cyclic Nucleotide Res 2 79-87 （1976）.
3 Wu, C., Ko, F., Kuo, S., et al. YC-1 inhibited human plaet aggregation through NO-independent activation of soluble guanylate cyclase. Br J Pharmacol 116 1973-1978 （1995）.
4 Arima, T., Ohshima, Y., Mizuno, T., et al. Cyclic GMP elevation by 5-hydroxytryptamine is due to nitric oxide derived from endogenous nitrosothiol in NG108-15 cells. Biochem Biophys Res Commun 227 473-478 （1996）.
5 Kolb, J.P., Paul-Eugene, N., Damais, C., et al. Interleukin-4 stimulates cGMP production by IFN-γ-activated human monocytes. Involvement of the nitric oxide synthase pathway. J Biol Chem 269 9811-9816 （1994）.
Synonyms Guanosine 3',5'-cyclic mononucleotide EIA Kit
Cyclic GMP ELISA Kit
cGMP EIA Kit
Guanosine 3',5'-cyclic monophosphate EIA Kit
Stability 1 year
Storage -20°C