Recombinant Protein Phosphatase 2, Regulatory Subunit B'' Alpha (PPP2R3a) 

蛋白磷酸酶2調節因子亞基B''α(PPP2R3a)重組蛋白

[ PROPERTIES ]

Source: Prokaryotic expression

Host: E.coli

Residues:  Asp827~Glu1150

Tags: N-terminal His Tag

Subcellular Location: Membrane, Plasma

Purity: > 95%

Traits: Freeze-dried powder

Buffer formulation: PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.

Original Concentration: 100µg

Applications: Positive Control; Immunogen; SDS-PAGE; WB.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 4.5

Predicted Molecular Mass:  41.7kDa

Accurate Molecular Mass:  46kDa as determined by SDS-PAGE reducing conditions.

Phenomenon explanation:

The possible reasons that the actual band size differs from the predicted are as follows:

1.Splice variants: Alternative splicing may create different sized proteins from the same gene.

2. Relative charge: The composition of amino acids may affects the charge of the protein.

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedto give the active form.

5. Polymerization of the target protein: Dimerization, multimerization etc.

[ USAGE ]

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

           Store at 2-8oC for one month.

           Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

[SEQUENCE ]

[  IDENTIFICATION  ]

[ IMPORTANT NOTE ]

The kit is designed for research use only, we will not be responsible for any issue if the kit was used in clinical diagnostic or any other procedures.

特別提示:本公司的所有產品僅可用于科研實驗,嚴禁用于臨床醫療及其他非科研用途!以實際收貨產品說明書為準,網站說明書僅供參考！