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化工儀器網(wǎng)>產(chǎn)品展廳>試劑標(biāo)物>生化試劑>抗體/抗原>7076S CST Anti-mouse IgG, HRP-linked Anti...

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7076S CST Anti-mouse IgG, HRP-linked Antibody

參考價 1500
訂貨量 ≥1
具體成交價以合同協(xié)議為準(zhǔn)

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上海盈東生物科技有限公司是一家專注于生命科學(xué)領(lǐng)域研究,集生物技術(shù)和產(chǎn)品開發(fā)、技術(shù)咨詢以及技術(shù)服務(wù)為一體的綜合性企業(yè)。公司目前可以提供生化試劑、分子生物學(xué)試劑及試劑盒、各種抗體及免疫學(xué)試劑盒、實驗耗材、科研儀器等多門類上萬種產(chǎn)品,為美國omegascientific品牌法國colcom品牌中國區(qū)總代理。公司推出“InnDonn”品牌多品類產(chǎn)品,堅持以品質(zhì)為主導(dǎo),科學(xué)為創(chuàng)新,為生命科學(xué)盡心盡責(zé)。


動物血清,胎牛血清,生化試劑,培養(yǎng)試劑,抗體,樹枝狀大分子多聚賴氨酸

供貨周期 一周 規(guī)格 1ml
貨號 7076S

Product Usage Information

Recommended Antibody Dilutions:

1:1000–1:3000

20X LumiGLO® Reagent and 20X Peroxide #7003 1:1K–1:3K

SignalFireTM ECL Reagent #6883 1:1K–1:3K

SignalFireTM Plus ECL Reagent #12630 1:5K-1:15K

SignalFireTM Elite ECL Reagent #12757 1:10K-1:20K

Storage

Supplied in 10mM sodium HEPES (pH 7.5), 150 mM NaCl, 2 mg/ml bovine serum albumin (BSA) and 50% glycerol. Store at –20°C. Do not aliquot the antibodies. This product is stable for 60 months when stored at -20C.

Product Description

Affinity purified horse anti-mouse IgG (heavy and light chain) antibody is conjugated to horseradish peroxidase(HRP) for chemiluminescent detection.  This product is thoroughly validated with CST primary antibodies and will work optimally with the CST western immunoblotting protocol, ensuring accurate and reproducible results.

Background

Chemiluminescence systems have emerged as the best all-around method for western blot detection. They eliminate the hazards associated with radioactive materials and toxic chromogenic substrates. The speed and sensitivity of these methods are unequalled by traditional alternatives, and because results are generated on film, it is possible to record and store data permanently. Blots detected with chemiluminescent methods are easily stripped for subsequent reprobing with additional antibodies. HRP-conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent substrates to generate the light signal. HRP conjugates have a very high turnover rate, yielding good sensitivity with short reaction times.

    Species Reactivity

    Species reactivity is determined by testing in at least one approved application (e.g., western blot).

    Western Blot Buffer

    IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

    Applications Key

    WB: Western Blotting

    Cross-Reactivity Key

    H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected


    僅限科研使用

    For Research Use Only. Not for Use in Diagnostic Procedures.
    Applications:

    WB

    Source/Isotype:

    Horse











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